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Candidatus Glomeribacter Gigasporarum
''Candidatus "''Glomeribacter gigasporarum" is a gram-negative β-proteobacteria. The bacterium is rod-shaped, and has a obligate endosymbiotic relationship with the arbuscular-mycorrhizal fungi Gigaspora margarita. Sequencing of the16S rRNA gene places ''Ca.'' "G. gigasporarum" within the Burkholderia ''Burkholderia'' is a genus of Pseudomonadota whose pathogenic members include the ''Burkholderia cepacia'' complex, which attacks humans and ''Burkholderia mallei'', responsible for glanders, a disease that occurs mostly in horses and related ... genus. ''Ca. "G. gigasporarum'' is unculturable as of yet, but can stay alive in enrichment for up to 4 weeks. The candidate bacteria is considered "the smallest beta-proteobacterium" with a genome size of 1.4 Mb. The chromosome is 750 kb long and a plasmid is 600 to 650 kb. The genome size was determined using gel-electrophoresis. Symbiotic relationship The relationship between ''Ca. "G. gigasporarum"'' and the fungi Gigaspora ma ...
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Gram-negative Bacteria
Gram-negative bacteria are bacteria that do not retain the crystal violet stain used in the Gram staining method of bacterial differentiation. They are characterized by their cell envelopes, which are composed of a thin peptidoglycan cell wall sandwiched between an inner cytoplasmic cell membrane and a bacterial outer membrane. Gram-negative bacteria are found in virtually all environments on Earth that support life. The gram-negative bacteria include the model organism ''Escherichia coli'', as well as many pathogenic bacteria, such as ''Pseudomonas aeruginosa'', '' Chlamydia trachomatis'', and ''Yersinia pestis''. They are a significant medical challenge as their outer membrane protects them from many antibiotics (including penicillin), detergents that would normally damage the inner cell membrane, and lysozyme, an antimicrobial enzyme produced by animals that forms part of the innate immune system. Additionally, the outer leaflet of this membrane comprises a complex lipopol ...
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Betaproteobacteria
Betaproteobacteria are a class of Gram-negative bacteria, and one of the eight classes of the phylum Pseudomonadota (synonym Proteobacteria). The ''Betaproteobacteria'' are a class comprising over 75 genera and 400 species of bacteria. Together, the ''Betaproteobacteria'' represent a broad variety of metabolic strategies and occupy diverse environments from obligate pathogens living within host organisms to oligotrophic groundwater ecosystems. Whilst most members of the ''Betaproteobacteria'' are heterotrophic, deriving both their carbon and electrons from organocarbon sources, some are photoheterotrophic, deriving energy from light and carbon from organocarbon sources. Other genera are autotrophic, deriving their carbon from bicarbonate or carbon dioxide and their electrons from reduced inorganic ions such as nitrite, ammonium, thiosulfate or sulfide — many of these chemolithoautotrophic. ''Betaproteobacteria'' are economically important, with roles in maintaining soil p ...
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Endosymbiont
An ''endosymbiont'' or ''endobiont'' is any organism that lives within the body or cells of another organism most often, though not always, in a mutualistic relationship. (The term endosymbiosis is from the Greek: ἔνδον ''endon'' "within", σύν ''syn'' "together" and βίωσις ''biosis'' "living".) Examples are nitrogen-fixing bacteria (called rhizobia), which live in the root nodules of legumes, single-cell algae inside reef-building corals and bacterial endosymbionts that provide essential nutrients to insects. There are two types of symbiont transmissions. In horizontal transmission, each new generation acquires free living symbionts from the environment. An example is the nitrogen-fixing bacteria in certain plant roots. Vertical transmission takes place when the symbiont is transferred directly from parent to offspring. It is also possible for both to be involved in a mixed-mode transmission, where symbionts are transferred vertically for some generation bef ...
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Arbuscular Mycorrhiza
An arbuscular mycorrhiza (AM) (plural ''mycorrhizae'', a.k.a. ''endomycorrhiza'') is a type of mycorrhiza in which the symbiont fungus (''AM fungi'', or AMF) penetrates the cortical cells of the roots of a vascular plant forming arbuscules. (Not to be confused with ectomycorrhiza or ericoid mycorrhiza.) Arbuscular mycorrhizae are characterized by the formation of unique structures, arbuscules and vesicles, by Glomeromycota and Mucoromycota, sister clades of the more well-known and diverse dikaryan fungi (all three are together called "symbiomycota"). AM fungi help plants to capture nutrients such as phosphorus, sulfur, nitrogen and micronutrients from the soil. It is believed that the development of the arbuscular mycorrhizal symbiosis played a crucial role in the initial colonisation of land by plants and in the evolution of the vascular plants. It has been said that it is quicker to list the plants that do not form endomycorrhizae than those that do. This symbiosis is a ...
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Burkholderia
''Burkholderia'' is a genus of Pseudomonadota whose pathogenic members include the ''Burkholderia cepacia'' complex, which attacks humans and ''Burkholderia mallei'', responsible for glanders, a disease that occurs mostly in horses and related animals; ''Burkholderia pseudomallei'', causative agent of melioidosis; and '' Burkholderia cepacia'', an important pathogen of pulmonary infections in people with cystic fibrosis (CF). ''Burkholderia'' species is also found marine environment. S.I. Paul et al. (2021) isolated and characterized ''Burkholderia cepacia'' from marine sponges of the Saint Martin's Island of the Bay of Bengal, Bangladesh. The ''Burkholderia'' (previously part of ''Pseudomonas'') genus name refers to a group of virtually ubiquitous Gram-negative, obligately aerobic, rod-shaped bacteria that are motile by means of single or multiple polar flagella, with the exception of ''Burkholderia mallei'', which is nonmotile. Members belonging to the genus do not produce s ...
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Gel Electrophoresis
Gel electrophoresis is a method for separation and analysis of biomacromolecules ( DNA, RNA, proteins, etc.) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge. Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving. Proteins are separated by the charge in agarose because the pores of the gel are too small to sieve proteins. Gel electrophoresis can also be used for the separation of nanoparticles. ...
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Symbiosis
Symbiosis (from Greek , , "living together", from , , "together", and , bíōsis, "living") is any type of a close and long-term biological interaction between two different biological organisms, be it mutualistic, commensalistic, or parasitic. The organisms, each termed a symbiont, must be of different species. In 1879, Heinrich Anton de Bary defined it as "the living together of unlike organisms". The term was subject to a century-long debate about whether it should specifically denote mutualism, as in lichens. Biologists have now abandoned that restriction. Symbiosis can be obligatory, which means that one or more of the symbionts depend on each other for survival, or facultative (optional), when they can generally live independently. Symbiosis is also classified by physical attachment. When symbionts form a single body it is called conjunctive symbiosis, while all other arrangements are called disjunctive symbiosis."symbiosis." Dorland's Illustrated Medical Dictionary. ...
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Burkholderiales
The Burkholderiales are an order of Pseudomonadota.George M. Garrity: '' Bergey's Manual of Systematic Bacteriology''. 2. Auflage. Springer, New York, 2005, Vol. 2: ''The Proteobacteria Part C: The Alpha-, Beta-, Delta-, and Epsilonproteabacteria'' Like all Pseudomonadota, they are Gram-negative. They include several pathogenic bacteria, including species of ''Burkholderia'', ''Bordetella'', and ''Ralstonia''. They also include '' Oxalobacter'' and related genera, which are unusual in using oxalic acid as their source of carbon. Other well-studied genera include ''Alcaligenes'', '' Cupriavidus'', ''Achromobacter'', ''Comamonas'', '' Delftia'', ''Massilia'', '' Duganella'', '' Janthinobacterium'', ''Polynucleobacter'' (important freshwater bacterioplankton), non-pathogenic ''Paraburkholderia'', '' Caballeronia'', '' Polaromonas'', ''Thiomonas'', ''Collimonas'', '' Hydrogenophaga'', ''Sphaerotilus'', '' Variovorax'', '' Acidovorax'', '' Rubrivivax'' and ''Rhodoferax'' (both members ...
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