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Bradford Protein Assay
The Bradford protein assay was developed by Marion M. Bradford in 1976. It is a quick and accurate spectroscopic analytical procedure used to measure the concentration of protein in a solution. The reaction is dependent on the amino acid composition of the measured proteins. Principle The Bradford assay, a colorimetric protein assay, is based on an absorbance shift of the dye Coomassie brilliant blue G-250. The Coomassie brilliant blue G-250 dye exists in three forms: anionic (blue), neutral (green), and cationic (red). Under acidic conditions, the red form of the dye is converted into its blue form, binding to the protein being assayed. If there's no protein to bind, then the solution will remain brown. The dye forms a strong, noncovalent complex with the protein's carboxyl group by van der Waals force and amino group through electrostatic interactions. During the formation of this complex, the red form of Coomassie dye first donates its free electron to the ionizable group ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bradford Protein Assay
The Bradford protein assay was developed by Marion M. Bradford in 1976. It is a quick and accurate spectroscopic analytical procedure used to measure the concentration of protein in a solution. The reaction is dependent on the amino acid composition of the measured proteins. Principle The Bradford assay, a colorimetric protein assay, is based on an absorbance shift of the dye Coomassie brilliant blue G-250. The Coomassie brilliant blue G-250 dye exists in three forms: anionic (blue), neutral (green), and cationic (red). Under acidic conditions, the red form of the dye is converted into its blue form, binding to the protein being assayed. If there's no protein to bind, then the solution will remain brown. The dye forms a strong, noncovalent complex with the protein's carboxyl group by van der Waals force and amino group through electrostatic interactions. During the formation of this complex, the red form of Coomassie dye first donates its free electron to the ionizable group ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Marion M
Marion may refer to: People *Marion (given name) *Marion (surname) *Marion Silva Fernandes, Brazilian footballer known simply as "Marion" * Marion (singer), Filipino singer-songwriter and pianist Marion Aunor (born 1992) Places Antarctica * Marion Nunataks, Charcot Island Australia * City of Marion, a local government area in South Australia * Marion, South Australia, a suburb of Adelaide Cyprus * Marion, Cyprus, an ancient city-state South Africa *Marion Island, one of the Prince Edward Islands United States * Marion, Alabama * Marion, Arkansas * Marion, Connecticut ** Marion Historic District (Cheshire and Southington, Connecticut) * Marion, Georgia * Marion, Illinois * Marion, Indiana, Grant County * Marion, Shelby County, Indiana * Marion, Iowa * Marion, Kansas ** Marion County Lake ** Marion Reservoir * Marion, Kentucky * Marion, Louisiana * Marion, Massachusetts * Marion Station, Maryland, often referred to as just "Marion" * Marion, Michigan * Marion, Minnesota * Mario ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sodium Dodecyl Sulfate
Sodium dodecyl sulfate (SDS) or sodium lauryl sulfate (SLS), sometimes written sodium laurilsulfate, is an organic compound with the formula . It is an anionic surfactant used in many cleaning and hygiene products. This compound is the sodium salt of the 12-carbon an organosulfate. Its hydrocarbon tail combined with a polar " headgroup" give the compound amphiphilic properties that make it useful as a detergent. SDS is also component of mixtures produced from inexpensive coconut and palm oils. SDS is a common component of many domestic cleaning, personal hygiene and cosmetic, pharmaceutical, and food products, as well as of industrial and commercial cleaning and product formulations. Physicochemical properties The critical micelle concentration (CMC) in water at 25 °C is 8.2 mM, and the aggregation number at this concentration is usually considered to be about 62. The micelle ionization fraction (α) is around 0.3 (or 30%). Applications Cleaning and hygiene SDS is main ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Biuret Protein Assay
Biuret is a chemical compound with the chemical formula . It is a white solid that is soluble in hot water. A variety of organic derivatives are known. The term "biuret" also describes a family of organic compounds with the chemical formula , where are hydrogen, organyl or other groups. Also known as carbamylurea, it results from the condensation of two equivalents of urea. As such, it is an undesirable impurity in urea-based fertilizers. As biuret is toxic to plants, its percentage in fertilizers must be kept low. Preparation and structure The parent compound can be prepared by heating urea at 150 °C for ~6 hours until it gets slightly cloudy, then recrystallizing from water. After that, it can be recrystallized repeatedly from 2% sodium hydroxide solution and water to finally get base-free crystalline needles of the monohydrate which are free of cyanuric acid. While heating, a lot of ammonia is expelled: : Under related conditions, pyrolysis of urea affords triuret . In general ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Ultraviolet–visible Spectroscopy
UV spectroscopy or UV–visible spectrophotometry (UV–Vis or UV/Vis) refers to absorption spectroscopy or reflectance spectroscopy in part of the ultraviolet and the full, adjacent visible regions of the electromagnetic spectrum. Being relatively inexpensive and easily implemented, this methodology is widely used in diverse applied and fundamental applications. The only requirement is that the sample absorb in the UV-Vis region, i.e. be a chromophore. Absorption spectroscopy is complementary to fluorescence spectroscopy. Parameters of interest, besides the wavelength of measurement, are absorbance (A) or transmittance (%T) or reflectance (%R), and its change with time. Optical transitions Most molecules and ions absorb energy in the ultraviolet or visible range, i.e., they are chromophores. The absorbed photon excites an electron in the chromophore to higher energy molecular orbitals, giving rise to an excited state. For organic chromophores, four possible types of transitions ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Normalization Of Concentration
Normalization or normalisation refers to a process that makes something more normal or regular. Most commonly it refers to: * Normalization (sociology) or social normalization, the process through which ideas and behaviors that may fall outside of social norms come to be regarded as "normal" Normalization or normalisation may also refer to: Science * Normalization process theory, a sociological theory of the implementation of new technologies or innovations * Normalization model, used in visual neuroscience * Normalization in quantum mechanics: see Mathematics and statistics * Normalization of an algebraic variety, the operation consisting in taking locally the integral closure of the ring of regular functions * Normalization (statistics), adjustments of values or distributions in statistics ** Quantile normalization, statistical technique for making two distributions identical in statistical properties * Normalizing (abstract rewriting), an abstract rewriting system in w ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
BSA Standard Curve
BSA may refer to: Businesses and organizations * Basketball South Africa * Bearing Specialists Association * Belarusian Socialist Assembly * Bibliographical Society of America * Birmingham Small Arms Company, UK manufacturer of firearms and vehicles * Black Socialists in America * Boston Society of Architects * Botanical Society of America * Boy Scouts of America **Scouts BSA, the flagship program * British Social Attitudes Survey * British Sandwich Association * British Science Association * British Sociological Association * British Speleological Association * British Stammering Association * Broadcasting Service Association, former name of the Australian radio network Macquarie Media * Broadcasting Standards Authority * BSA Company, motorcycle manufacturer * BSA motorcycles, made by the Birmingham Small Arms Company Limited * BSA (The Software Alliance), a trade group established by Microsoft, formerly called Business Software Alliance * Business Services Association, of UK ser ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Linear Regression
In statistics, linear regression is a linear approach for modelling the relationship between a scalar response and one or more explanatory variables (also known as dependent and independent variables). The case of one explanatory variable is called '' simple linear regression''; for more than one, the process is called multiple linear regression. This term is distinct from multivariate linear regression, where multiple correlated dependent variables are predicted, rather than a single scalar variable. In linear regression, the relationships are modeled using linear predictor functions whose unknown model parameters are estimated from the data. Such models are called linear models. Most commonly, the conditional mean of the response given the values of the explanatory variables (or predictors) is assumed to be an affine function of those values; less commonly, the conditional median or some other quantile is used. Like all forms of regression analysis, linear regression focuses on ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bovine Serum Albumin
Bovine serum albumin (BSA or "Fraction V") is a serum albumin protein derived from cows. It is often used as a protein concentration standard in lab experiments. The nickname "Fraction V" refers to albumin being the fifth fraction of the original Edwin Cohn purification methodology that made use of differential solubility characteristics of plasma proteins. By manipulating solvent concentrations, pH, salt levels, and temperature, Cohn was able to pull out successive "fractions" of blood plasma. The process was first commercialized with human albumin for medical use and later adopted for production of BSA. Properties The full-length BSA precursor polypeptide is 607 amino acids (AAs) in length. An N-terminal 18-residue signal peptide is cut off from the precursor protein upon secretion, hence the initial protein product contains 589 amino acid residues. An additional six amino acids are cleaved to yield the mature BSA protein that contains 583 amino acids. BSA has thre ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Spectronic 20
The Spectronic 20 is a brand of single-beam spectrophotometer, designed to operate in the visible spectrum across a wavelength range of 340 Nanometer, nm to 950 nm, with a Spectral linewidth, spectral bandpass of 20 nm. It is designed for Quantitative analysis (chemistry), quantitative absorption measurement at single wavelengths. Because it measures the transmittance or Absorption (electromagnetic radiation), absorption of visible light through a Solution (chemistry), solution, it is sometimes referred to as a Colorimeter (chemistry), colorimeter. The name of the instrument is a trademark of the manufacturer. Developed by Bausch & Lomb and launched in 1953, the Spectronic 20 was the first low-cost spectrophotometer. It rapidly became an industry standard due to its low cost, durability and ease of use, and has been referred to as an "iconic lab spectrophotometer". Approximately 600,000 units were sold over its nearly 60 year production run. It has been the most widely ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Phosphoric Acid
Phosphoric acid (orthophosphoric acid, monophosphoric acid or phosphoric(V) acid) is a colorless, odorless phosphorus-containing solid, and inorganic compound with the chemical formula . It is commonly encountered as an 85% aqueous solution, which is a colourless, odourless, and non- volatile syrupy liquid. It is a major industrial chemical, being a component of many fertilizers. The compound is an acid. Removal of all three ions gives the phosphate ion . Removal of one or two protons gives dihydrogen phosphate ion , and the hydrogen phosphate ion , respectively. Phosphoric acid forms esters, called organophosphates. The name "orthophosphoric acid" can be used to distinguish this specific acid from other "phosphoric acids", such as pyrophosphoric acid. Nevertheless, the term "phosphoric acid" often means this specific compound; and that is the current IUPAC nomenclature. Production Phosphoric acid is produced industrially by one of two routes, wet processes and dry. We ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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