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Aniline Blue WS
Aniline Blue WS, also called aniline blue, diphenylamine blue, China blue, or Soluble blue, is a mixture of methyl blue and water blue. It may also be either one of them. It is a soluble dye used as a biological dye, in fluorescence microscopy, appearing a yellow-green colour after excitation with violet light. It is a mixture of the trisulfonates of triphenyl rosaniline and of diphenyl rosaniline. Aniline blue or its constituents are used to stain collagen, as the fibre stain in Masson's trichrome, as well as to reveal callose structures in plant tissues. It can be used in the Mallory's connective tissue stain and Gömöri trichrome stain. It is used in differential staining Differential staining is a staining process which uses more than one chemical stain. Using multiple stains can better differentiate between different microorganisms or structures/cellular components of a single organism. Differential staining is u .... References {{DEFAULTSORT:Aniline Blue Ws Stai ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Water Blue
Water blue, also known as aniline blue, Acid blue 22, Soluble Blue 3M, Marine Blue V, or C.I. 42755, is a chemical compound used as a stain in histology. Water blue stains collagen blue in tissue sections. It is soluble in water and slightly soluble in ethanol. Water blue is also available in mixture with methyl blue, under the names Aniline Blue WS, Aniline blue, China blue, or Soluble blue. It can be used in the Mallory's trichrome stain of connective tissue and Gömöri trichrome stain of muscle tissue. It is used in differential staining Differential staining is a staining process which uses more than one chemical stain. Using multiple stains can better differentiate between different microorganisms or structures/cellular components of a single organism. Differential staining is u .... See also * RAL 5021 Water blue References {{Reflist Triarylmethane dyes Staining dyes Benzenesulfonates Organic sodium salts Anilines Acid dyes ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Methyl Blue
Methyl blue is a chemical compound with the molecular formula C37H27N3Na2O9S3. It is used as a stain in histology, and stains collagen blue in tissue sections. It can be used in some differential staining techniques such as Mallory's connective tissue stain and Gömöri trichrome stain, and can be used to mediate electron transfer in microbial fuel cells. Fungal cell walls are also stained by methyl blue. Methyl blue is also available in mixture with water blue, under name Aniline Blue WS, Aniline blue, China blue, or Soluble blue; and in a solution of phenol, glycerol, and lactic acid under the name Lactophenol cotton blue (LPCB), which is used for microscopic visualization of fungi. Chemistry Methyl blue ( 4-[Bis[4-[(sulfophenyl)aminohenyl">is[4-[(sulfophenyl)amino.html" ;"title="4-[Bis[4-[(sulfophenyl)amino">4-[Bis[4-[(sulfophenyl)aminohenylethylene]-2,5-cyclohexadien-1-ylidene]amino]-benzenesulfonic acid disodium salt) is distinctly different to methylene blue ([7- ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Diphenylamine
Diphenylamine is an organic compound with the formula (C6H5)2NH. The compound is a derivative of aniline, consisting of an amine bound to two phenyl groups. The compound is a colorless solid, but commercial samples are often yellow due to oxidized impurities.P. F. Vogt, J. J. Gerulis, "Amines, Aromatic" in Ullmann’s Encyclopedia of Industrial Chemistry 2005, Wiley-VCH, Weinheim. Diphenylamine dissolves well in many common organic solvents, and is moderately soluble in water. It is used mainly for its antioxidant properties. Diphenylamine is widely used as an industrial antioxidant, dye mordant and reagent and is also employed in agriculture as a fungicide and antihelmintic. Preparation and reactivity Diphenylamine is manufactured by the thermal deamination of aniline over oxide catalysts: : 2 C6H5NH2 → (C6H5)2NH + NH3 It is a weak base, with a ''K''b of 10−14. With strong acids, it forms salts. For example, treatment with sulfuric acid gives the bisulfate C6H5)2NH ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Methyl Blue
Methyl blue is a chemical compound with the molecular formula C37H27N3Na2O9S3. It is used as a stain in histology, and stains collagen blue in tissue sections. It can be used in some differential staining techniques such as Mallory's connective tissue stain and Gömöri trichrome stain, and can be used to mediate electron transfer in microbial fuel cells. Fungal cell walls are also stained by methyl blue. Methyl blue is also available in mixture with water blue, under name Aniline Blue WS, Aniline blue, China blue, or Soluble blue; and in a solution of phenol, glycerol, and lactic acid under the name Lactophenol cotton blue (LPCB), which is used for microscopic visualization of fungi. Chemistry Methyl blue ( 4-[Bis[4-[(sulfophenyl)aminohenyl">is[4-[(sulfophenyl)amino.html" ;"title="4-[Bis[4-[(sulfophenyl)amino">4-[Bis[4-[(sulfophenyl)aminohenylethylene]-2,5-cyclohexadien-1-ylidene]amino]-benzenesulfonic acid disodium salt) is distinctly different to methylene blue ([7- ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Water Blue
Water blue, also known as aniline blue, Acid blue 22, Soluble Blue 3M, Marine Blue V, or C.I. 42755, is a chemical compound used as a stain in histology. Water blue stains collagen blue in tissue sections. It is soluble in water and slightly soluble in ethanol. Water blue is also available in mixture with methyl blue, under the names Aniline Blue WS, Aniline blue, China blue, or Soluble blue. It can be used in the Mallory's trichrome stain of connective tissue and Gömöri trichrome stain of muscle tissue. It is used in differential staining Differential staining is a staining process which uses more than one chemical stain. Using multiple stains can better differentiate between different microorganisms or structures/cellular components of a single organism. Differential staining is u .... See also * RAL 5021 Water blue References {{Reflist Triarylmethane dyes Staining dyes Benzenesulfonates Organic sodium salts Anilines Acid dyes ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorescence Microscope
A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances. "Fluorescence microscope" refers to any microscope that uses fluorescence to generate an image, whether it is a simple set up like an epifluorescence microscope or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescence image. Principle The specimen is illuminated with light of a specific wavelength (or wavelengths) which is absorbed by the fluorophores, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light). The illumination light is separated from the much weaker emitted fluorescence through the use of a spectral emission filter. Typical components of a fluorescence microscope are a light source (xenon arc lamp or mercury-vapor lamp are ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Staining (biology)
Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissues), in cytology (microscopic study of cells), and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of diseases at the microscopic level. Stains may be used to define biological tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells), or organelles within individual cells. In biochemistry, it involves adding a class-specific ( DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis. Light microscopes are used for viewing st ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Collagen
Collagen () is the main structural protein in the extracellular matrix found in the body's various connective tissues. As the main component of connective tissue, it is the most abundant protein in mammals, making up from 25% to 35% of the whole-body protein content. Collagen consists of amino acids bound together to form a triple helix of elongated fibril known as a collagen helix. It is mostly found in connective tissue such as cartilage, bones, tendons, ligaments, and skin. Depending upon the degree of mineralization, collagen tissues may be rigid (bone) or compliant (tendon) or have a gradient from rigid to compliant (cartilage). Collagen is also abundant in corneas, blood vessels, the gut, intervertebral discs, and the dentin in teeth. In muscle tissue, it serves as a major component of the endomysium. Collagen constitutes one to two percent of muscle tissue and accounts for 6% of the weight of the skeletal muscle tissue. The fibroblast is the most common cell that crea ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Masson's Trichrome
Masson's trichrome is a three-colour staining procedure used in histology. The recipes evolved from Claude L. Pierre Masson's (1880–1959) original formulation have different specific applications, but all are suited for distinguishing cells from surrounding connective tissue. Most recipes produce red keratin and muscle fibers, blue or green collagen and bone, light red or pink cytoplasm, and dark brown to black cell nuclei. The trichrome is applied by immersion of the fixated sample into Weigert's iron hematoxylin, and then three different solutions, labeled A, B, and C: * Weigert's hematoxylin is a sequence of three solutions: ferric chloride in diluted hydrochloric acid, hematoxylin in 95% ethanol, and potassium ferricyanide solution alkalized by sodium borate. It is used to stain the nuclei. * Solution A, also called plasma stain, contains acid fuchsin, Xylidine Ponceau, glacial acetic acid, and distilled water. Other red acid dyes can be used, e.g. the Biebrich sc ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Connective Tissue
Connective tissue is one of the four primary types of animal tissue, along with epithelial tissue, muscle tissue, and nervous tissue. It develops from the mesenchyme derived from the mesoderm the middle embryonic germ layer. Connective tissue is found in between other tissues everywhere in the body, including the nervous system. The three meninges, membranes that envelop the brain and spinal cord are composed of connective tissue. Most types of connective tissue consists of three main components: elastic and collagen fibers, ground substance, and cells. Blood, and lymph are classed as specialized fluid connective tissues that do not contain fiber. All are immersed in the body water. The cells of connective tissue include fibroblasts, adipocytes, macrophages, mast cells and leucocytes. The term "connective tissue" (in German, ''Bindegewebe'') was introduced in 1830 by Johannes Peter Müller. The tissue was already recognized as a distinct class in the 18th century. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gömöri Trichrome Stain
Gömöri trichrome stain is a histological stain used on muscle tissue. It can be used to test for certain forms of mitochondrial myopathy. It is named for George Gömöri George Gomori may refer to: * György Gömöri (1904–1957), Hungarian-American physician and histochemist * George Gomori (born 1934), Hungarian-born poet, writer and academic {{hndis, Gomori, George ..., who developed it in 1950.GOMORI, G. - A rapid one-step trichrome stain. Am. J. Clin. Path. 20: 661-664, 1950. References External links Staining 1950 introductions {{pathology-stub ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Differential Staining
Differential staining is a staining process which uses more than one chemical staining, stain. Using multiple stains can better differentiate between different microorganisms or structures/cellular components of a single organism. Differential staining is used to detect abnormalities in the proportion of different white blood cells in the blood. The process or results are called a WBC differential. This test is useful because many diseases alter the proportion of certain white blood cells. By analyzing these differences in combination with a clinical exam and other lab tests, medical professionals can diagnose disease. One commonly recognizable use of differential staining is the Gram stain. Gram staining uses two dyes: Crystal violet and Fuchsin or Safranin (the counterstain) to differentiate between Gram-positive bacteria (large Peptidoglycan layer on outer surface of cell) and Gram-negative bacteria. Acid-fast stains are also differential stains. Further reading *http://ww ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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