Aniline Blue WS, also called aniline blue,
diphenylamine
Diphenylamine is an organic compound with the formula (C6H5)2NH. The compound is a derivative of aniline, consisting of an amine bound to two phenyl groups. The compound is a colorless solid, but commercial samples are often yellow due to oxidiz ...
blue, China blue, or Soluble blue, is a mixture of
methyl blue
Methyl blue is a chemical compound with the molecular formula C37H27N3Na2O9S3. It is used as a stain in histology, and stains collagen blue in tissue sections. It can be used in some differential staining techniques such as Mallory's connect ...
and
water blue. It may also be either one of them. It is a soluble dye used as a biological dye, in
fluorescence microscopy
A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances. "Fluorescence microscop ...
, appearing a yellow-green colour after excitation with violet light. It is a mixture of the trisulfonates of triphenyl rosaniline and of diphenyl rosaniline.
Aniline blue or its constituents are used to
stain collagen, as the fibre stain in
Masson's trichrome,
as well as to reveal callose structures in plant tissues.
It can be used in the Mallory's
connective tissue stain
and
Gömöri trichrome stain
Gömöri trichrome stain is a histological stain used on muscle tissue.
It can be used to test for certain forms of mitochondrial myopathy.
It is named for George Gömöri George Gomori may refer to:
* György Gömöri (1904–1957), Hungari ...
. It is used in
differential staining Differential staining is a staining process which uses more than one chemical stain. Using multiple stains can better differentiate between different microorganisms or structures/cellular components of a single organism.
Differential staining is u ...
.
References
{{DEFAULTSORT:Aniline Blue Ws
Staining dyes
Triarylmethane dyes