Subtilisin

Subtilisin is a protease (a protein-digestingenzyme) initially obtained from Bacillus subtilis.

Subtilisins belong to subtilases, a group of serine proteases that – like all serine proteases – initiate the nucleophilic attack on the peptide (amide) bond through a serine residue at the active site. Subtilisins typically have molecular weights 27kDa. They can be obtained from certain types of soilbacteria, for example, Bacillus amyloliquefaciens from which they are secreted in large amounts.