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Plasmid

Small DNA molecule within a cellA plasmid is a circular DNA molecule, separate from the genome, that can replicate inside of bacteria. This allows scientists to copy, multiply, and express a gene of interest.A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently.

Extrachromosomal DNAExtrachromosomal DNAExtrachromosomal DNA (abbreviated ecDNA) is any DNA that is found off the chromosomes, either inside or outside the nucleus of a cell. Most DNA in an individual genome is found in chromosomes contained in the nucleus. Multiple forms of extrachromosomal DNA exist, and, while some of these serve important biological functions, they can also play a role in diseases such as cancer.Vector (molecular biology)Vector (molecular biology)In molecular cloning, a vector is any particle (e.g., plasmids, cosmids, Lambda phages) used as a vehicle to artificially carry a foreign nucleic sequence – usually DNA – into another cell, where it can be replicated and/or expressed. A vector containing foreign DNA is termed recombinant DNA. The four major types of vectors are plasmids, viral vectors, cosmids, and artificial chromosomes. Of these, the most commonly used vectors are plasmids.Molecular cloningMolecular cloningMolecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms. The use of the word cloning refers to the fact that the method involves the replication of one molecule to produce a population of cells with identical DNA molecules.VirulenceVirulence is a pathogen's or microorganism's ability to cause damage to a host. In most cases, especially in animal systems, virulence refers to the degree of damage caused by a microbe to its host. The pathogenicity of an organism—its ability to cause disease—is determined by its virulence factors. In the specific context of gene for gene systems, often in plants, virulence refers to a pathogen's ability to infect a resistant host.BacteriaBacteriaDomain of microorganismsSynonyms"Bacteria" (Cohn 1872) Cavalier-Smith 1983"Bacteria" Haeckel 1894"Bacteria" Cavalier-Smith 2002"Bacteriaceae" Cohn 1872a"Bacteriobionta" Möhn 1984"Bacteriophyta" Schussnig 1925"Eubacteria" Woese and Fox 1977"Neobacteria" Möhn 1984"Schizomycetaceae" de Toni and Trevisan 1889"Schizomycetes" Nägeli 1857Bacteria are ubiquitous, mostly free-living organisms often consisting of one biological cell.ArchaeaArchaeaDomain of organismsExcluded(but cladistically included)EukaryotaSynonyms"Archaebacteria" Woese & Fox 1977"Archaeobacteria" Murray 1988"Mendocutes" Gibbons & Murray 1978"Mendosicutes" Murray 1984"Metabacteria" Hori and Osawa 1979"Neomura" Cavalier-Smith 2002"Archaebiota" Luketa 2012"Arkarya" Forterre 2015Archaea ( ) is a domain of organisms.EukaryoteEukaryoteDomain of life whose cells have nucleiThe eukaryotes () are cellularorganisms of the domainEukaryota or Eukarya, which have a distinct cell nucleus (formed by a nuclear membrane), membrane-bound organelles and organization supported by a cytoskeleton. Extant eukaryotes are traditionally grouped into four kingdoms: animals, plants, fungi and protists.DNA constructA DNA construct is an artificially-designed segment of DNA borne on a vector that can be used to incorporate genetic material into a target tissue or cell. A DNA construct contains a DNA insert, called a transgene, delivered via a transformation vector which allows the insert sequence to be replicated and/or expressed in the target cell. This gene can be cloned from a naturally occurring gene, or synthetically constructed.BioremediationBioremediationProcess used to treat contaminated media such as water and soil Part of a series onPollutionAir pollution from a factory Air Acid rain Air quality index Atmospheric dispersion modeling Chlorofluorocarbon Combustion Exhaust gas Haze Household air pollution Global dimming Global distillation Indoor air quality Non-exhaust emissions Ozone depletion Particulate matter Persistent organic pollutant Smog Soot Volatile organic compound Biological Biological hazard Genetic Illegal logging Introduced species Invasive species Digital Information Effects of violence in mass media Electromagnetic Light Ecological Overillumination Radio spectrum Natural Ozone Radium and radon in the environment Volcanic ash Wildfire Noise Transportation Health effects from noise Marine mammals and sonar Noise barrier Noise control Soundproofing Radiation Actinides Bioremediation Depleted uranium Nuclear fission Nuclear fallout Plutonium Poisoning Radioactivity Uranium Radioactive waste Soil Agricultural Land degradation Bioremediation Defecation Electrical resistance heating Illegal mining Soil guideline values Phytoremediation Solid waste Advertising mail Biodegradable waste Brown waste Electronic waste Food waste Green waste Hazardous waste Industrial waste Landfill Litter Mining Municipal solid waste Nanomaterials Plastic Packaging waste Post-consumer waste Waste management Space Space debris Thermal Urban heat island Visual Air travel Advertising clutter Overhead power lines Traffic signs Urban blight Vandalism War Chemical warfare Herbicidal warfare Agent Orange Nuclear holocaust Nuclear fallout Nuclear famine Nuclear winter Scorched earth Unexploded ordnance War and environmental law Water Agricultural wastewater Biosolids Diseases Eutrophication Firewater Freshwater Groundwater Hypoxia Industrial wastewater Marine Monitoring Nonpoint source Nutrient Ocean acidification Oil spill Pharmaceuticals Freshwater salinization Septic tanks Sewage Shipping Sludge Stagnation Sulfur water Surface runoff Turbidity Urban runoff Water quality Wastewater Topics History Pollutants Heavy metals Paint Misc Area source Brain health and pollution Debris Dust Garbology Legacy Midden Point source Technofossil Waste Toxic Lists Diseases Law by country Most polluted cities Least polluted cities by PM2.5 Treaties Most polluted rivers Categories By country Environment portal Ecology portalvte Bioremediation broadly refers to any process wherein a biological system (typically bacteria, microalgae, fungi in mycoremediation, and plants in phytoremediation), living or dead, is employed for removing environmental pollutants from air, water, soil, fuel gasses, industrial effluents etc., in natural or artificial settings.Antimicrobial resistanceAntimicrobial resistanceAntimicrobial resistance (AMR or AR) occurs when microbes evolve mechanisms that protect them from antimicrobials, which are drugs used to treat infections in humans, animals, and plants. Any microbe can develop resistance, including bacteria (antibiotic resistance), viruses (antiviral resistance), parasites (antiparasitic resistance), and fungi (antifungal resistance).Secondary metabolismSecondary metabolismIn biochemistry, secondary metabolism (also called specialized metabolism) is a term for pathways and small molecule products of metabolism that are involved in ecological interactions, but are not absolutely required for the survival of the organism. These molecules are sometimes produced by specialized cells, such as laticifers in plants.Genomic DNAGenomic DNAGenomic deoxyribonucleic acid (abbreviated as gDNA) is chromosomal DNA, in contrast to extra-chromosomal DNAs like plasmids. Most organisms have the same genomic DNA in every cell; however, only certain genes are active in each cell to allow for cell function and differentiation within the body. gDNA predominantly resides in the cell nucleus packed into dense chromosome structures. Chromatin refers to the combination of DNA and proteins that make up chromosomes.

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