Bioluminescence imagingBioluminescence imaging (BLI) is a technology developed over the past decades (1990's and onward). that allows for the noninvasive study of ongoing biological processes Recently, bioluminescence tomography (BLT) has become possible and several systems have become commercially available. In 2011, PerkinElmer acquired one of the most popular lines of optical imaging systems with bioluminescence from Caliper Life Sciences.
EnzymeLarge biological molecule that acts as a catalystThe enzyme glucosidase converts the sugar maltose into two glucose sugars. Active site residues in red, maltose substrate in black, and NADcofactor in yellow. (PDB: 1OBB)An enzyme is a biological macromolecule, usually a protein, that acts as a biological catalyst, accelerating chemical reactions without being consumed in the process.
BioluminescenceBioluminescence is the production of light by an organism as the result of a chemiluminescence reaction. It occurs in a wide variety of organisms, including marine vertebrates and invertebrates, terrestrial arthropods such as fireflies, some fungi, and microorganisms such as some bacteria and dinoflagellates.
LuciferinLuciferin (from Latin lucifer 'light-bearer') is a generic term for the light-emitting compound found in organisms that generate bioluminescence. Luciferins typically undergo an enzyme-catalyzed reaction with molecular oxygen. The resulting transformation, which usually involves breaking off a molecular fragment, produces an excited state intermediate that emits light upon decaying to its ground state.
PhotoproteinIn molecular biology, photoproteins are a type of enzyme produced by bioluminescent organisms. They add to the function of the luciferins whose usual light-producing reaction is catalyzed by the enzyme luciferase. HistoryThe term photoprotein was first used to describe the unusual chemistry of the luminescent system of Chaetopterus (a marine Polychaete worm).
Raphaël DuboisRaphaël Horace Dubois (20 June 1849 – 21 January 1929) was a French pharmacologist known for his work on bioluminescence and anesthesia. He coined the terms proteon and bioproteon, from the Greek "proteon" for matter and "bios" for life. Bioproteon means "living matter". He concluded that there was no difference between matter and living matter.
Reporter geneReporter genes are molecular tools widely used in molecular biology, genetics, and biotechnology to study gene function, expression patterns, and regulatory mechanisms. These genes encode proteins that produce easily detectable signals, such as fluorescence, luminescence, or enzymatic activity, allowing researchers to monitor cellular processes in real-time.
Molecular biologyMolecular biology is a branch of biology that seeks to understand the molecular structures and chemical processes that are the basis of biological activity within and between cells. It is centered largely on the study of nucleic acids (such as DNA and RNA) and proteins.Fluorescent proteinFluorescent proteins include: Green fluorescent protein (GFP) Yellow fluorescent protein (YFP) Red fluorescent protein (RFP)
FluorescenceFluorescence is one of two kinds of photoluminescence, the emission of light by a substance that has absorbed light or other electromagnetic radiation. When exposed to ultraviolet radiation, many substances will glow (fluoresce) with colored visible light. The color of the light emitted depends on the chemical composition of the substance. Fluorescent materials generally cease to glow nearly immediately when the radiation source stops.
BiotechnologyField of science that uses cells for the creation of productsA biologist conducting research in a biotechnology laboratoryBiotechnology is a multidisciplinary field that involves the integration of natural sciences and engineering sciences to achieve the application of organisms and parts thereof for products and services. The term biotechnology was first used by Károly Ereky in 1919 to refer to the production of products from raw materials with the aid of living organisms.
-aseThe suffix-ase is used in biochemistry to form names of enzymes. The most common way to name enzymes is to add this suffix onto the end of the substrate, e.g. an enzyme that breaks down peroxides may be called peroxidase; the enzyme that produces telomeres is called telomerase. Sometimes enzymes are named for the function they perform, rather than substrate, e.g. the enzyme that polymerizes (assembles) DNA into strands is called polymerase; see also reverse transcriptase.*As an Amazon Associate I earn from qualifying purchases.