RNA-SeqRNA-Seq (short for RNA sequencing) is a next-generation sequencing (NGS) technique used to quantify and identify RNA molecules in a biological sample, providing a snapshot of the transcriptome at a specific time. It enables transcriptome-wide analysis by sequencing cDNA derived from RNA. Modern workflows often incorporate pseudoalignment tools (such as Kallisto and Salmon) and cloud-based processing pipelines, improving speed, scalability, and reproducibility.3' mRNA-seq3' mRNA-seq is a quantitative, genome-wide transcriptomic technique based on the barcoding of the 3' untranslated region (UTR) of mRNA molecules. Unlike standard bulk RNA-seq, where short sequencing reads are generated along the entire length of mRNA transcripts, only the 3' end of polyadenylated RNAs are sequenced in 3' mRNA-seq.
Unique molecular identifierUnique molecular identifiers (UMIs), or molecular barcodes (MBC) are short sequences or molecular "tags" added to DNA fragments in some next generation sequencing library preparation protocols to identify the input DNA molecule. These tags are added before PCR amplification, and can be used to reduce errors and quantitative bias introduced by the amplification.
Peer reviewPeer review is the evaluation of work by one or more people with similar competencies as the producers of the work (peers). It functions as a form of self-regulation by qualified members of a profession within the relevant field. Peer review methods are used to maintain quality standards, improve performance, and provide credibility. In academia, scholarly peer review is typically used to determine an academic paper's suitability for publication.Genome ResearchGenome Research is a peer-reviewed scientific journal published by Cold Spring Harbor Laboratory Press. The journal scope covers genome biology, including genome structure, function and evolution, comparative genomics, molecular evolution, genome-scale quantitative and population genetics, proteomics, epigenomics, and systems biology, genomic medicine, and single-cell analysis.
École Polytechnique Fédérale de LausanneEPFL (official name, initialism for French: École Polytechnique Fédérale de Lausanne, lit.'Federal School of Technology in Lausanne') is a publicresearch university in Lausanne, Switzerland. Founded in 1969, the university primarily teaches and conducts research in science, technology, engineering, and mathematics (STEM) fields.
Real-time polymerase chain reactionA real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used quantitatively and semi-quantitatively (i.e., above/below a certain amount of DNA molecules).
Illumina, Inc.Illumina, Inc. is an American biotechnology company, headquartered in San Diego, California. Incorporated on April 1, 1998, Illumina develops, manufactures, and markets integrated systems for the analysis of genetic variation and biological function. The company provides a line of products and services that serves the sequencing, genotyping and gene expression, and proteomics markets, and serves more than 155 countries.
MGI (company)MGI or MGI Tech is a Chinese biotechnology company, which provides a line of products and technologies that serves the genetic sequencing, genotyping and gene expression, and proteomics markets. Its headquarters are located in Shenzhen, Guangdong, China. HistoryIn 2016, MGI was founded as a subsidiary of BGI Group. It manufactures high-throughput genetic sequencing systems and other products for use in the life sciences and health care sectors.
Massively parallel sequencingMassively parallel sequencing (MPS) is any of several high-throughput approaches to DNA sequencing using the concept of massively parallel processing; it is also called next-generation sequencing (NGS) or second-generation sequencing. Some of these technologies emerged between 1993 and 1998 and have been commercially available since 2005.
RNAFamily of large biological moleculesA hairpin loop from a pre-mRNA. Highlighted are the nucleobases (green) and the ribose-phosphate backbone (blue). This is a single strand of RNA that folds back upon itself. Ribonucleic acid (RNA) is a polymeric molecule that is essential for most biological functions, either by performing the function itself (non-coding RNA) or by forming a template for the production of proteins (messenger RNA).
Coverage (genetics)In genetics, coverage is one of several measures of the depth or completeness of DNA sequencing, and is more specifically expressed in any of the following terms: Sequence coverage (or depth) is the number of unique reads that include a given nucleotide in the reconstructed sequence.*As an Amazon Associate I earn from qualifying purchases.