biochemistry Biochemistry, or biological chemistry, is the study of chemical processes within and relating to living organisms. A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology, a ...

and

molecular biology Molecular biology is a branch of biology that seeks to understand the molecule, molecular basis of biological activity in and between Cell (biology), cells, including biomolecule, biomolecular synthesis, modification, mechanisms, and interactio ...

, saline-sodium citrate (SSC) buffer is used as a hybridization

buffer Buffer may refer to: Science * Buffer gas, an inert or nonflammable gas * Buffer solution, a solution used to prevent changes in pH * Lysis buffer, in cell biology * Metal ion buffer * Mineral redox buffer, in geology Technology and engineeri ...

, to control stringency for washing steps in protocols for Southern blotting,

in situ hybridization ''In situ'' hybridization (ISH) is a type of Hybridisation (molecular biology), hybridization that uses a labeled complementary DNA, RNA or modified nucleic acid strand (i.e., a Hybridization probe, probe) to localize a specific DNA or RNA seq ...

DNA Microarray A DNA microarray (also commonly known as a DNA chip or biochip) is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the expression levels of large numbers of genes simultaneously or t ...

or Northern blotting. 20X SSC may be used to prevent drying of agarose gels during a vacuum transfer. A 20X stock solution consists of 3 M

sodium chloride Sodium chloride , commonly known as Salt#Edible salt, edible salt, is an ionic compound with the chemical formula NaCl, representing a 1:1 ratio of sodium and chloride ions. It is transparent or translucent, brittle, hygroscopic, and occurs a ...

and 300 mM

trisodium citrate Trisodium citrate is a chemical compound with the molecular formula Na3C6H5O7. It is sometimes referred to simply as " sodium citrate", though sodium citrate can refer to any of the three sodium salts of citric acid. It possesses a saline, mild ...

(adjusted to pH 7.0 with HCl).

References

Material safety data sheet for 20x SSC buffer, Mediatech, Inc.

Buffer solutions {{biochem-stub

See also

References