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Western Blot
The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. Besides detecting the proteins, this technique is also utilized to visualize, distinguish, and quantify the different proteins in a complicated protein combination. Western blot technique uses three elements to achieve its task of separating a specific protein from a complex: separation by size, transfer of protein to a solid support, and marking target protein using a primary and secondary antibody to visualize. A synthetic or animal-derived antibody (known as the primary antibody) is created that recognizes and binds to a specific target protein. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognizes and binds to the primary antibody ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Western Blot Workflow
Western may refer to: Places *Western, Nebraska, a village in the US *Western, New York, a town in the US *Western Creek, Tasmania, a locality in Australia *Western Junction, Tasmania, a locality in Australia *Western world, countries that identify with shared "Western" culture Arts and entertainment Films *Western (1997 film), ''Western'' (1997 film), a French road movie directed by Manuel Poirier *Western (2017 film), ''Western'' (2017 film), a German-Austrian film Genres *Western (genre), a category of fiction and visual art centered on the American Old West **Western fiction, the Western genre as featured in literature **Western music (North America), a type of American folk music Music *Westerns (EP), ''Westerns'' (EP), an EP by Pete Yorn *WSTRN, a British hip hop group from west London Business *The Western, a closed hotel/casino in Las Vegas, United States *Western Cartridge Company, a manufacturer of ammunition *Western Publishing, a defunct publishing company Edu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
