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Thermophoresis
Thermophoresis (also thermomigration, thermodiffusion, the Soret effect, or the Ludwig–Soret effect) is a phenomenon observed in mixtures of mobile particles where the different particle types exhibit different responses to the force of a temperature gradient. This phenomenon tends to move light molecules to hot regions and heavy molecules to cold regions. The term ''thermophoresis'' most often applies to aerosol mixtures whose mean free path \lambda is comparable to its characteristic length scale L, but may also commonly refer to the phenomenon in all phases of matter. The term ''Soret effect'' normally applies to liquid mixtures, which behave according to different, less well-understood mechanisms than gaseous mixtures. Thermophoresis may not apply to thermomigration in solids, especially multi-phase alloys. Thermophoretic force The phenomenon is observed at the scale of one millimeter or less. An example that may be observed by the naked eye with good lighting is when the hot ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Microscale Thermophoresis
Microscale thermophoresis (MST) is a technology for the Biophysics, biophysical analysis of interactions between biomolecules. Microscale thermophoresis is based on the detection of a temperature-induced change in fluorescence of a target as a function of the concentration of a non-fluorescent ligand. The observed change in fluorescence is based on two distinct effects. On the one hand it is based on a temperature related intensity change (TRIC) of the fluorescent probe, which can be affected by binding events. On the other hand, it is based on thermophoresis, the directed movement of particles in a microscopic temperature gradient. Any change of the chemical microenvironment of the fluorescent probe, as well as changes in the solvation shell#Hydration shells of proteins, hydration shell of biomolecules result in a relative change of the fluorescence detected when a temperature gradient is applied and can be used to determine dissociation constant, binding affinities. MST allows me ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
