Cuvette
In laboratories, a cuvette () is a small tube-like container with straight sides and a circular or square cross-section. It is sealed at one end, and made of a clear, transparent material such as plastic, glass, or fused quartz. Cuvettes are designed to hold samples for spectroscopic measurement, where a beam of light is passed through the sample within the cuvette to measure the absorbance, transmittance, fluorescence intensity, fluorescence polarization, or fluorescence lifetime of the sample. This measurement is done with a spectrophotometer. Overview Traditional ultraviolet–visible spectroscopy or fluorescence spectroscopy uses samples that are liquid. Often the sample is a solution, with the substance of interest dissolved within. The sample is placed in a cuvette and the cuvette is placed in a spectrophotometer for testing. The cuvette can be made of any material that is transparent in the range of wavelengths used in the test. The smallest cuvettes can hold 70&n ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Cuvette With Penny
In laboratory, laboratories, a cuvette () is a small tube-like container with straight sides and a circular or square cross-section. It is sealed at one end, and made of a clear, transparent material such as plastic, glass, or fused quartz. Cuvettes are designed to hold samples for Spectroscopy, spectroscopic measurement, where a beam of light is passed through the sample within the cuvette to measure the absorbance, transmittance, fluorescence spectroscopy, fluorescence intensity, fluorescence anisotropy, fluorescence polarization, or fluorescence lifetime of the sample. This measurement is done with a Spectrophotometry, spectrophotometer. Overview Traditional ultraviolet–visible spectroscopy or fluorescence spectroscopy uses samples that are liquid. Often the sample is a Solution (chemistry), solution, with the substance of interest dissolved within. The sample is placed in a cuvette and the cuvette is placed in a spectrophotometer for testing. The cuvette can be made of ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Spectrophotometry
Spectrophotometry is a branch of electromagnetic spectroscopy concerned with the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength. Spectrophotometry uses photometers, known as spectrophotometers, that can measure the intensity of a light beam at different wavelengths. Although spectrophotometry is most commonly applied to ultraviolet, Visible spectrum, visible, and infrared radiation, modern spectrophotometers can interrogate wide swaths of the electromagnetic spectrum, including x-ray, ultraviolet, Visible spectrum, visible, infrared, or microwave wavelengths. Overview Spectrophotometry is a tool that hinges on the quantitative analysis of molecules depending on how much light is absorbed by colored compounds. Important features of spectrophotometers are spectral bandwidth (the range of colors it can transmit through the test sample), the percentage of sample transmission, the logarithmic range of sample absorptio ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Fluorescence Spectroscopy
Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light, that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily, visible light. A complementary technique is absorption spectroscopy. In the special case of single molecule fluorescence spectroscopy, intensity fluctuations from the emitted light are measured from either single fluorophores, or pairs of fluorophores. Devices that measure fluorescence are called fluorometers. Theory Molecules have various states referred to as energy levels. Fluorescence spectroscopy is primarily concerned with electronic and vibrational states. Generally, the species being examined has a ground electronic state (a low energy state) of interest, and an excited electronic state of higher energy. Within each of these ele ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Circular Dichroism
Circular dichroism (CD) is dichroism involving circular polarization, circularly polarized light, i.e., the differential Absorption (electromagnetic radiation), absorption of left- and right-handed light. Left-hand circular (LHC) and right-hand circular (RHC) polarized light represent two possible spin angular momentum of light, spin angular momentum states for a photon, and so circular dichroism is also referred to as dichroism for spin angular momentum. This phenomenon was discovered by Jean-Baptiste Biot, Augustin Fresnel, and Aimé Cotton in the first half of the 19th century. Circular dichroism and optical rotation, circular birefringence are manifestations of optical activity. It is exhibited in the absorption (electromagnetic radiation), absorption bands of optical activity, optically active chirality (chemistry), chiral molecules. CD spectroscopy has a wide range of applications in many different fields. Most notably, Ultraviolet, far-UV CD is used to investigate the second ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Assay
An assay is an investigative (analytic) procedure in laboratory medicine, mining, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity. The measured entity is often called the analyte, the measurand, or the target of the assay. The analyte can be a drug, biochemical substance, chemical element or compound, or cell in an organism or organic sample. An assay usually aims to measure an analyte's intensive property and express it in the relevant measurement unit (e.g. molarity, density, functional activity in enzyme international units, degree of effect in comparison to a standard, etc.). If the assay involves exogenous reactants (the reagents), then their quantities are kept fixed (or in excess) so that the quantity and quality of the target are the only limiting factors. The difference in the assay outcome is used to deduce the unknown quality ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Absorbance
Absorbance is defined as "the logarithm of the ratio of incident to transmitted radiant power through a sample (excluding the effects on cell walls)". Alternatively, for samples which scatter light, absorbance may be defined as "the negative logarithm of one minus absorptance, as measured on a uniform sample". The term is used in many technical areas to quantify the results of an experimental measurement. While the term has its origin in quantifying the absorption of light, it is often entangled with quantification of light which is "lost" to a detector system through other mechanisms. What these uses of the term tend to have in common is that they refer to a logarithm of the ratio of a quantity of light incident on a sample or material to that which is detected after the light has interacted with the sample. The term absorption refers to the physical process of absorbing light, while absorbance does not always measure only absorption; it may measure attenuation (of transmitted ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Transmittance
Electromagnetic radiation can be affected in several ways by the medium in which it propagates. It can be Scattering, scattered, Absorption (electromagnetic radiation), absorbed, and Fresnel equations, reflected and refracted at discontinuities in the medium. This page is an overview of the last 3. The transmittance of a material and any surfaces is its effectiveness in transmitting radiant energy; the fraction of the initial (incident) radiation which propagates to a location of interest (often an observation location). This may be described by the transmission coefficient. Surface Transmittance Hemispherical transmittance Hemispherical transmittance of a surface, denoted ''T'', is defined as :T = \frac, where *Φet is the radiant flux ''transmitted'' by that surface into the hemisphere on the opposite side from the incident radiation; *Φei is the radiant flux received by that surface. Hemispheric transmittance may be calculated as an integral over the directional trans ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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DNS Containing Solution In A Cuvette
The Domain Name System (DNS) is a hierarchical and distributed name service that provides a naming system for computers, services, and other resources on the Internet or other Internet Protocol (IP) networks. It associates various information with ''domain names'' (identification strings) assigned to each of the associated entities. Most prominently, it translates readily memorized domain names to the numerical IP addresses needed for locating and identifying computer services and devices with the underlying network protocols. The Domain Name System has been an essential component of the functionality of the Internet since 1985. The Domain Name System delegates the responsibility of assigning domain names and mapping those names to Internet resources by designating authoritative name servers for each domain. Network administrators may delegate authority over subdomains of their allocated name space to other name servers. This mechanism provides distributed and fault-tolerant ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Acetone
Acetone (2-propanone or dimethyl ketone) is an organic compound with the chemical formula, formula . It is the simplest and smallest ketone (). It is a colorless, highly Volatile organic compound, volatile, and flammable liquid with a characteristic pungent odor. Acetone is miscibility, miscible with properties of water, water and serves as an important organic solvent in industry, home, and laboratory. About 6.7 million tonnes were produced worldwide in 2010, mainly for use as a solvent and for production of methyl methacrylate and bisphenol A, which are precursors to widely used plastics.Acetone World Petrochemicals report, January 2010Stylianos Sifniades, Alan B. Levy, "Acetone" in Ullmann's Encyclopedia of Industrial Chemistry, Wiley-VCH, Weinheim, 2005. It is a common building block in organic chemistry. ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Gauze
Gauze is a thin, translucent Textile, fabric with a wikt:loose, loose open Weaving, weave. In technical terms, "gauze" is a weave structure in which the weft yarns are arranged in pairs and are crossed before and after each Warp (weaving), warp yarn, keeping the weft firmly in place. This weave structure is used to add stability to the fabric, which is important when using fine yarns loosely spaced. However, this weave structure can be used with any weight of yarn, and can be seen in some rustic textiles made from coarse hand-spun plant fiber yarns. Gauze is widely used for medical dressings. Gauze can also be made of non-woven fabric. Etymology and history The word ''gauze'' came into English in the 16th century via French ''gaze'', beyond which its history is uncertain. Most scholars trace ''gauze'' to a Persian language, Persian word for thin cloth or an Arabic word for raw silk. The Philip III, Bishop of Fermo#Hungary, 1279 Council of Baden banned clergy from wearing " ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Detergent
A detergent is a surfactant or a mixture of surfactants with Cleanliness, cleansing properties when in Concentration, dilute Solution (chemistry), solutions. There are a large variety of detergents. A common family is the alkylbenzene sulfonates, which are soap-like compounds that are more soluble than soap in hard water, because the polar sulfonate is less likely than the polar carboxylate of soap to bind to calcium and other ions found in hard water. Definitions The word ''detergent'' is derived from the Latin adjective , from the verb , meaning to wipe or polish off. Detergent can be defined as a surfactant or a mixture of surfactants with cleansing properties when in Concentration, dilute Solution (chemistry), solutions. However, conventionally, detergent is used to mean synthetic cleaning compounds as opposed to ''soap'' (a salt of the natural fatty acid), even though soap is also a detergent in the true sense. In domestic contexts, the term ''detergent'' refers to househ ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |
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Ethanol
Ethanol (also called ethyl alcohol, grain alcohol, drinking alcohol, or simply alcohol) is an organic compound with the chemical formula . It is an Alcohol (chemistry), alcohol, with its formula also written as , or EtOH, where Et is the pseudoelement symbol for ethyl group, ethyl. Ethanol is a Volatility (chemistry), volatile, flammable, colorless liquid with a characteristic wine-like odor and pungent taste. As a psychoactive depressant, it is the active ingredient in alcoholic beverages, and the second most consumed drug globally behind caffeine. Ethanol is naturally produced by the fermentation process of sugars by yeasts or via petrochemical processes such as ethylene hydration. Historically it was used as a general anesthetic, and has modern medical applications as an antiseptic, disinfectant, solvent for some medications, and antidote for methanol poisoning and ethylene glycol poisoning. It is used as a chemical solvent and in the Chemical synthesis, synthesis of orga ... [...More Info...]       [...Related Items...]     OR:     [Wikipedia]   [Google]   [Baidu]   |