assay

An assay is an investigative (analytic) procedure in laboratory medicine, mining, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity. The measured entity is often called the analyte, the measurand, or the target of the assay. The analyte can be a drug, biochemical substance, chemical element or compound, or cell in an organism or organic sample. An assay usually aims to measure an analyte's intensive property and express it in the relevant measurement unit (e.g. molarity, density, functional activity in enzyme international units, degree of effect in comparison to a standard, etc.).

If the assay involves exogenous reactants (the reagents), then their quantities are kept fixed (or in excess) so that the quantity and quality of the target are the only limiting factors. The difference in the assay outcome is used to deduce the unknown quality or quantity of the target in question. Some assays (e.g., biochemical assays) may be similar to chemical analysis and titration. However, assays typically involve biological material or phenomena that are intrinsically more complex in composition or behavior, or both. Thus, reading of an assay may be noisy and involve greater difficulties in interpretation than an accurate chemical titration. On the other hand, older generation qualitative assays, especially bioassays, may be much more gross and less quantitative (e.g., counting death or dysfunction of an organism or cells in a population, or some descriptive change in some body part of a group of animals).

Assays have become a routine part of modern medical, environmental, pharmaceutical, and forensic technology. Other businesses may also employ them at the industrial, curbside, or field levels. Assays in high commercial demand have been well investigated in research and development sectors of professional industries. They have also undergone generations of development and sophistication. In some cases, they are protected by intellectual property regulations such as patents granted for inventions. Such industrial-scale assays are often performed in well-equipped laboratories and with automated organization of the procedure, from ordering an assay to pre-analytic sample processing (sample collection, necessary manipulations e.g. spinning for separation, aliquoting if necessary, storage, retrieval, pipetting, aspiration, etc.). Analytes are generally tested in high- throughput autoanalyzers, and the results are verified and automatically returned to ordering service providers and end-users. These are made possible through the use of an advanced laboratory informatics system that interfaces with multiple computer terminals with end-users, central servers, the physical autoanalyzer instruments, and other automata.

Etymology

According to Etymology Online, the verb ''assay'' means "to try, endeavor, strive, test the quality of"; from Anglo-French ''assaier'', from ''assai'' (noun), from Old French ''essai'', "trial". Thus the noun ''assay'' means "trial, test of quality, test of character" (from mid-14th century), from Anglo-French ''assai''; and its meaning "analysis" is from the late 14th century.

For assay of currency coins this literally meant analysis of the purity of the gold or silver (or whatever the precious component) that represented the true value of the coin. This might have translated later (possibly after the 14th century) into a broader usage of "analysis", e.g., in pharmacology, analysis for an important component of a target inside a mixture—such as the active ingredient of a drug inside the inert excipients in a formulation that previously was measured only grossly by its observable action on an organism (e.g., a lethal dose or inhibitory dose).

General steps

An assay (analysis) is never an isolated process, as it must be accompanied with pre- and post-analytic procedures. Both the communication order (the request to perform an assay plus related information) ''and'' the handling of the specimen itself (the collecting, documenting, transporting, and processing done before beginning the assay) are pre-analytic steps. Similarly, after the assay is completed the results must be documented, verified and communicated—the post-analytic steps. As with any multi-step information handling and transmission system, the variation and errors in reporting final results entail not only those intrinsic to the assay itself but also those occurring in the pre-analytic and post-analytic procedures.

While the analytic steps of the assay itself get much attention, it is those that get less attention of the chain of users—the pre-analytic and post-analytic procedures—that typically accumulate the most errors; e.g., pre-analytic steps in medical laboratory assays may contribute 32–75% of all lab errors.

Assays can be very diverse, but generally involve the following general steps:
# Sample processing and manipulation in order to selectively present the target in a discernible or measurable form to a discrimination/identification/detection system. It might involve a simple centrifugal separation or washing or filtration or capture by some form of selective binding or it may even involve modifying the target e.g. epitope retrieval in immunological assays or cutting down the target into pieces e.g. in Mass Spectrometry. Generally there are multiple separate steps done before an assay and are called preanalytic processing. But some of the manipulations may be inseparable part of the assay itself and will not thus be considered pre-analytic.
# Target-specific discrimination/identification principle: to discriminate from background (noise) of similar components and specifically identify a particular target component ("analyte") in a biological material by its specific attributes. (e.g. in a PCR assay a specific oligonucleotide primer identifies the target by base pairing based on the specific nucleotide sequence unique to the target).
# Signal (or target) amplification system: The presence and quantity of that analyte is converted into a detectable signal generally involving some method of signal amplification, so that it can be easily discriminated from noise and measured - e.g. in a PCR assay among a mixture of DNA sequences only the specific target is amplified into millions of copies by a DNA polymerase enzyme so that it can be discerned as a more prominent component compared to any other potential components. Sometimes the concentration of the analyte is too large and in that case the assay may involve sample dilution or some sort of signal diminution system which is a negative amplification.
# Signal detection (and interpretation) system: A system of deciphering the amplified signal into an interpretable output that can be quantitative or qualitative. It can be visual or manual very crude methods or can be very sophisticated electronic digital or analog detectors.
#Signal enhancement and noise filtering may be done at any or all of the steps above. Since the more downstream a step/process during an assay, the higher the chance of carrying over noise from the previous process and amplifying it, multiple steps in a sophisticated assay might involve various means of signal-specific sharpening/enhancement arrangements and noise reduction or filtering arrangements. These may simply be in the form of a narrow band-pass optical filter, or a blocking reagent in a binding reaction that prevents nonspecific binding or a quenching reagent in a fluorescence detection system that prevents "autofluorescence" of background objects.

Assay types based on the nature of the assay process

Time and number of measurements taken

Depending on whether an assay just looks at a single time point or timed readings taken at multiple time points, an assay may be:
#An end point assay, in which a single measurement is performed after a fixed incubation period; or
#A kinetic assay, in which measurements are performed multiple times over a fixed time interval. Kinetic assay results may be visualized numerically (for example, as a slope parameter representing the rate of signal change over time), or graphically (for example, as a plot of the signal measured at each time point). For kinetic assays, both the magnitude and shape of the measured response over time provide important information.
#A high throughput assay can be either an endpoint or a kinetic assay usually done on an automated platform in 96-, 384- or 1536-well microplate formats (High Throughput Screening). Such assays are able to test large number of compounds or analytes or make functional biological readouts in response to a stimuli and/or compounds being tested.

Number of analytes detected

Depending on how many targets or analytes are being measured:
#Usual assays are simple or ''single target assays'' which is usually the default unless it is called multiplex.
# Multiplex assays are used to simultaneously measure the presence, concentration, activity, or quality of multiple analytes in a single test. The advent of ''multiplexing'' enabled rapid, efficient sample testing in many fields, including immunology, cytochemistry, genetics/genomics, pharmacokinetics, and toxicology.

Result type

Depending on the quality of the result produced, assays may be classified into:
# Qualitative assays, i.e. assays which generally give just a pass or fail, or positive or negative or some such sort of only small number of qualitative gradation rather than an exact quantity.
#Semi-quantitative assays, i.e. assays that give the read-out in an approximate fashion rather than an exact number for the quantity of the substance. Generally they have a few more gradations than just two outcomes, positive or negative, e.g. scoring on a scale of 1+ to 4+ as used for blood grouping tests based on RBC agglutination in response to grouping reagents (antibody against blood group antigens).
# Quantitative assays, i.e. assays that give accurate and exact numeric quantitative measure of the amount of a substance in a sample. An example of such an assay used in coagulation testing laboratories for the most common inherited bleeding disease - Von Willebrand disease is VWF antigen assay where the amount of VWF present in a blood sample is measured by an immunoassay.
# Functional assays, i.e. an assay that tries to quantify functioning of an active substance rather than just its quantity. The functional counterpart of the VWF antigen assay is Ristocetin Cofactor assay, which measures the functional activity of the VWF present in a patients plasma by adding exogenous formalin-fixed platelets and gradually increasing quantities of drug named ristocetin while measuring agglutination of the fixed platelets. A similar assay but used for a different purpose is called Ristocetin Induced Platelet Aggregation or RIPA, which tests response of endogenous live platelets from a patient in response to Ristocetin (exogenous) & VWF (usually endogenous).

Sample type and method

Depending on the general substrate on which the assay principle is applied:
#Bioassay: when the response is biological activity of live objects. Examples include
##''in vivo'', whole organism (e.g. mouse or other subject injected with a drug)
##''ex vivo'' body part (e.g. leg of a frog)
##''ex vivo'' organ (e.g. heart of a dog)
##''ex vivo'' part of an organ (e.g. a segment of an intestine).
##tissue (e.g. limulus lysate)
##cell (e.g. platelets)
# Ligand binding assay when a ligand (usually a small molecule) binds a receptor (usually a large protein).
# Immunoassay when the response is an antigen antibody binding type reaction.

Signal amplification

Depending on the nature of the signal amplification system assays may be of numerous types, to name a few:
# Enzyme assay: Enzymes may be tested by their highly repeating activity on a large number of substrates when loss of a substrate or the making of a product may have a measurable attribute like color or absorbance at a particular wavelength or light or Electrochemiluminescence or electrical/redox activity.
#Light detection systems that may use amplification e.g. by a photodiode or a photomultiplier tube or a cooled charge coupled device.
# Radioisotope labeled substrates as used in radioimmunoassays and equilibrium dialysis assays and can be detected by the amplification in Gamma counters or X-ray plates, or phosphorimager
#Polymerase Chain Reaction Assays that amplify a DNA (or RNA) target rather than the signal
#Combination Methods Assays may utilize a combination of the above and other amplification methods to improve sensitivity. e.g. Enzyme-linked immunoassay or EIA, enzyme linked immunosorbent assay.

Detection method or technology

Depending on the nature of the Detection system assays can be based on:
#Colony forming or virtual colony count: e.g. by multiplying bacteria or proliferating cells.
# Photometry / spectrophotometry When the absorbance of a specific wavelength of light while passing through a fixed path-length through a cuvette of liquid test sample is measured and the absorbance is compared with a blank and standards with graded amounts of the target compound. If the emitted light is of a specific visible wavelength it may be called colorimetry, or it may involve specific wavelength of light e.g. by use of laser and emission of fluorescent signals of another specific wavelength which is detected via very specific wavelength optical filters.
# Transmittance of light may be used to measure e.g. clearing of opacity of a liquid created by suspended particles due to decrease in number of clumps during a platelet agglutination reaction.
# Turbidimetry when the opacity of straight-transmitted light passing through a liquid sample is measured by detectors placed straight across the light source.
# Nephelometry where a measurement of the amount of light scattering that occurs when a beam of light is passed through the solution is used to determine size and/or concentration and/or size distribution of particles in the sample.
# Reflectometry When color of light reflected from a (usually dry) sample or reactant is assessed e.g. the automated readings of the strip urine dipstick assays.
#Viscoelastic measurements e.g. viscometry, elastography (e.g. thromboelastography)
#Counting assays: e.g. optic Flow cytometric cell or particle counters, or coulter/impedance principle based cell counters
#Imaging assays, that involve image analysis manually or by software:
## Cytometry: When the size statistics of cells is assessed by an image processor.
#Electric detection e.g. involving amperometry, Voltammetry, coulometry may be used directly or indirectly for many types of quantitative measurements.
#Other physical property based assays may use
## Osmometer
## Viscometer
##Ion Selective electrodes
##Syndromic testing

Assay types based on the targets being measured

DNA

Assays for studying interactions of proteins with DNA include:
* DNase footprinting assay
* Filter binding assay
* Gel shift assay

Protein

* Bicinchoninic acid assay (BCA assay)
* Bradford protein assay
* Lowry protein assay
* Secretion assay

RNA

*Nuclear run-on
*Ribosome profiling

Cell counting, viability, proliferation or cytotoxicity assays

A cell-counting assay may determine the number of living cells, the number of dead cells, or the ratio of one cell type to another, such as numerating and typing red versus different types of white blood cells. This is measured by different physical methods (light transmission, electric current change). But other methods use biochemical probing cell structure or physiology (stains). Another application is to monitor cell culture ( assays of cell proliferation or cytotoxicity).
A cytotoxicity assay measures how toxic a chemical compound is to cells.
* MTT assay
*Cell Counting Kit-8 (WST-8 based cell viability assay)
*SRB ( Sulforhodamine B) assay
*CellTiter-Glo® Luminescent Cell Viability Assay
*Cell counting instruments and methods: CASY cell counting technology, Coulter counter, Electric cell-substrate impedance sensing
*Cell viability assays: resazurin method, ATP test, Ethidium homodimer assay (detect dead or dying cells), Bacteriological water analysis, Clonogenic assays, ...

Environmental or food contaminants

* Bisphenol F
* Aquatic toxicity tests

Surfactants

*An MBAS assay indicates anionic surfactants in water with a bluing reaction.

Other cell assays

Many cell assays have been developed to assess specific parameters or response of cells (biomarkers, cell physiology). Techniques used to study cells include :
*reporter assays using i.e. Luciferase, calcium signaling assays using Coelenterazine, CFSE or Calcein
* Immunostaining of cells on slides by Microscopy ( ImmunoHistoChemistry or Fluorescence), on microplates by photometry including the ELISpot (and its variant FluoroSpot) to enumerate B-Cells or antigen-specific cells, in solution by Flow cytometry
*Molecular biology techniques such as DNA microarrays, in situ hybridization, combined to PCR