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MRC-5
MRC-5 ( Medical Research Council cell strain 5) is a diploid cell culture line composed of fibroblasts, originally developed from the lung tissue of a 14-week-old aborted white male fetus. The cell line was isolated by J.P. Jacobs and colleagues in September 1966 from the seventh population doubling of the original strain, and MRC-5 cells themselves are known to reach senescence in around 45 population doublings. Applications MRC-5 cells are currently used to produce several vaccines including for hepatitis A, varicella and polio. Culture and society During the COVID-19 pandemic, anti-vaccination and anti-abortion activists believed that MRC-5 was an ingredient of the Oxford–AstraZeneca COVID-19 vaccine, citing a study from the University of Bristol. David Matthews, a co-author for this study, clarified that MRC-5 was solely used for testing purposes to determine "how the Oxford vaccine behaves when it is inside a genetically normal human cell." The manufacturing of the vaccine ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Hepatitis A Vaccine
Hepatitis A vaccine is a vaccine that prevents hepatitis A. It is effective in around 95% of cases and lasts for at least twenty years and possibly a person's entire life. If given, two doses are recommended beginning after the age of one. It is given by injection into a muscle. The first hepatitis A vaccine was approved in the European Union in 1991, and the United States in 1995. It is on the World Health Organization's List of Essential Medicines. The World Health Organization (WHO) recommends universal vaccination in areas where the disease is moderately common. Where the disease is very common, widespread vaccination is not recommended as people typically develop immunity through infection during childhood. The US Centers for Disease Control and Prevention (CDC) recommends vaccinating: * All children aged 12–23 months * Unvaccinated children and adolescents aged 2–18 years * International travelers * Men who have sex with men * People who use injection or non-inject ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
WI-38
WI-38 is a diploid human cell line composed of fibroblasts derived from lung tissue of a 3-month-gestation female fetus. The fetus came from a legal abortion performed in Sweden in 1962. The cell line was isolated by Leonard Hayflick the same year, and has been used extensively in scientific research, with applications ranging from developing important theories in molecular biology and aging to the production of most human virus vaccines. The uses of this cell line in human virus vaccine production is estimated to have saved the lives of millions of people. History The WI-38 cell line stemmed from earlier work by Hayflick growing human cell cultures. In the early 1960s, Hayflick and his colleague Paul Moorhead at the Wistar Institute in Philadelphia, Pennsylvania discovered that when normal human cells were stored in a freezer, the cells remembered the doubling level at which they were stored and, when reconstituted, began to divide from that level to roughly 50 total doublings ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cell Culture
Cell culture or tissue culture is the process by which cell (biology), cells are grown under controlled conditions, generally outside of their natural environment. After cells of interest have been Cell isolation, isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator. These conditions vary for each cell type, but generally consist of a suitable vessel with a substrate or rich growth medium, medium that supplies the essential nutrients (amino acids, carbohydrates, vitamins, minerals), growth factors, hormones, and gases (Carbon dioxide, CO2, Oxygen, O2), and regulates the physio-chemical environment (Buffer solution, pH buffer, osmotic pressure, temperature). Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture. T ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Use Of Fetal Tissue In Vaccine Development
The use of fetal tissue in vaccine development is the practice of researching, developing, and producing vaccines through growing viruses in Cell culture, cultured (laboratory-grown) Cell (biology), cells that were originally derived from human Fetus, fetal tissue. Since the cell strains in use originate from abortions, there has been opposition to the practice and the resulting vaccines on religious and moral grounds. The vaccines do not contain any of the original fetal tissue or cells or cells derived from fetal materials. Although the vaccine materials are purified from cell debris, traces of human DNA fragments remain. The cell lines continue to replicate on their own and no further sources of fetal cells are needed. The Catholic Church and abortion, Catholic Church has encouraged its members to use alternative vaccines, produced without human cell lines, if possible. However, the Vatican has clarified that "all vaccinations recognized as clinically safe and effective can be ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
COVID-19 Pandemic
The COVID-19 pandemic (also known as the coronavirus pandemic and COVID pandemic), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), began with an disease outbreak, outbreak of COVID-19 in Wuhan, China, in December 2019. Soon after, it spread to other areas of Asia, and COVID-19 pandemic by country and territory, then worldwide in early 2020. The World Health Organization (WHO) declared the outbreak a public health emergency of international concern (PHEIC) on 30 January 2020, and assessed the outbreak as having become a pandemic on 11 March. COVID-19 symptoms range from asymptomatic to deadly, but most commonly include fever, sore throat, nocturnal cough, and fatigue. Transmission of COVID-19, Transmission of the virus is often airborne transmission, through airborne particles. Mutations have variants of SARS-CoV-2, produced many strains (variants) with varying degrees of infectivity and virulence. COVID-19 vaccines were developed rapidly and deplo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Kidney
In humans, the kidneys are two reddish-brown bean-shaped blood-filtering organ (anatomy), organs that are a multilobar, multipapillary form of mammalian kidneys, usually without signs of external lobulation. They are located on the left and right in the retroperitoneal space, and in adult humans are about in length. They receive blood from the paired renal artery, renal arteries; blood exits into the paired renal veins. Each kidney is attached to a ureter, a tube that carries excreted urine to the urinary bladder, bladder. The kidney participates in the control of the volume of various body fluids, fluid osmolality, Acid-base homeostasis, acid-base balance, various electrolyte concentrations, and removal of toxins. Filtration occurs in the glomerulus (kidney), glomerulus: one-fifth of the blood volume that enters the kidneys is filtered. Examples of substances reabsorbed are solute-free water, sodium, bicarbonate, glucose, and amino acids. Examples of substances secreted are hy ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
HEK 293 Cells
Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, are an immortalised cell line derived from HEK cells isolated from a female fetus in the 1970s. The HEK 293 cell line has been widely used in research for decades due to its reliable and fast growth and propensity for transfection. The cell line is used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy as well as safety testing for a vast array of chemicals. History HEK 293 cells were generated in 1973 by transfection of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in Lex van der Eb, Alex van der Eb's laboratory in Leiden, Leiden, the Netherlands. The cells were obtained from a single, aborted or miscarried fetus, the precise origin of which is unclear. The cells were cultured by van der Eb; the transfection with adenoviral DNA was performed by Frank L. Graham, Frank Graham, a Postdoctoral research, post-doc in van der ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Oxford–AstraZeneca COVID-19 Vaccine
The Oxford–AstraZeneca COVID19 vaccine, sold under the brand names Covishield and Vaxzevria among others, is a viral vector vaccine COVID-19 vaccine, for the prevention of COVID-19. It was developed in the United Kingdom by University of Oxford, Oxford University and British-Swedish company AstraZeneca, using as a vector the modified chimpanzee Adenoviridae, adenovirus ChAdOx1. The vaccine is given by intramuscular injection. Studies carried out in 2020 showed that the Vaccine efficacy, efficacy of the vaccine is 76.0% at preventing symptomatic COVID-19 beginning at 22 days following the first dose and 81.3% after the second dose. A study in Scotland found that, for symptomatic COVID-19 infection after the second dose, the vaccine is 81% effective against the SARS-CoV-2 Alpha variant, Alpha variant (lineage B.1.1.7) and 61% against the SARS-CoV-2 Delta variant, Delta variant (lineage B.1.617.2). The vaccine is stable at refrigerator temperatures and has a good safety prof ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Anti-abortion Movements
Anti-abortion movements, also self-styled as pro-life movements, are involved in the abortion debate advocating against the practice of abortion and its legality. Many anti-abortion movements began as countermovements in response to the legalization of elective abortions. Europe In Europe, abortion law varies by country, and has been legalized through parliamentary acts in some countries, and constitutionally banned or heavily restricted in others. In Western Europe this has had the effect at once of both more closely regulating the use of abortion, and at the same time mediating and reducing the impact anti-abortion campaigns have had on the law. France The first specifically anti-abortion organization in France, Laissez-les-vivre-SOS futures mères, was created in 1971 during the debate that was to lead to the Veil Law in 1975. Its main spokesman was the geneticist Jérôme Lejeune. Since 2005, the French anti-abortion movement has organized an annual March for Life ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
MERS Coronavirus Particles (14702511068)
Middle East respiratory syndrome (MERS) is a viral respiratory infection caused by '' Middle East respiratory syndrome–related coronavirus'' (MERS-CoV). Symptoms may range from none, to mild, to severe depending on age and risk level. Typical symptoms include fever, cough, diarrhea, and shortness of breath. The disease is typically more severe in those with other health problems. The first case was identified in June 2012 by Egyptian physician Ali Mohamed Zaki at the Dr. Soliman Fakeeh Hospital in Jeddah, Saudi Arabia, and most cases have occurred in the Arabian Peninsula. Over 2,600 cases have been reported as of January 2021, including 45 cases in the year 2020. About 35% of those who are diagnosed with the disease die from it. Larger outbreaks have occurred in South Korea in 2015 and in Saudi Arabia in 2018. MERS-CoV is a virus in the coronavirus family believed to be originally from bats. However, humans are typically infected from camels, either during direct ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Polio Vaccine
Polio vaccines are vaccines used to prevent poliomyelitis (polio). Two types are used: an inactivated vaccine, inactivated poliovirus given by injection (IPV) and a attenuated vaccine, weakened poliovirus given by mouth (OPV). The World Health Organization (WHO) recommends all children be fully vaccinated against polio. The two vaccines have eliminated polio from most of the world, and reduced the number of cases reported each year from an estimated 350,000 in 1988 to 33 in 2018. The inactivated polio vaccines are very safe. Mild redness or pain may occur at the site of injection. Oral polio vaccines cause about three cases of vaccine-associated paralytic poliomyelitis per million doses given. This compares with 5,000 cases per million who are paralysed following a polio infection. Both types of vaccine are generally safe to give during pregnancy and in those who have HIV/AIDS, but are otherwise well. However, the emergence of circulating vaccine-derived poliovirus (cVDPV), a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Medical Research Council (United Kingdom)
The Medical Research Council (MRC) is responsible for co-coordinating and funding medical research in the United Kingdom. It is part of United Kingdom Research and Innovation (UKRI), which came into operation 1 April 2018, and brings together the UK's seven research councils, Innovate UK and Research England. UK Research and Innovation is answerable to, although politically independent from, the Department for Business, Energy and Industrial Strategy. The MRC focuses on high-impact research and has provided the financial support and scientific expertise behind a number of medical breakthroughs, including the development of penicillin and the discovery of the structure of DNA. Research funded by the MRC has produced 32 Nobel Prize winners to date. History The MRC was founded as the Medical Research Committee and Advisory Council in 1913, with its prime role being the distribution of medical research funds under the terms of the National Insurance Act 1911. This was a conseq ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
