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Fluorescent Chloride Sensors
Fluorescent chloride sensors are used for chemical analysis. The discoveries of chloride (Cl−) participations in physiological processes stimulates the measurements of intracellular Cl− in live cells and the development of fluorescent tools referred below. Quinoline-based dyes quinolinium - based Cl− indicators are based on the capability of halides to quench the fluorescence of heterocyclic organic compounds with quaternary nitrogen. Fluorescence is quenched by a collision mechanism with a linear Stern–Volmer relationship: \frac = 1 + K( l^- where: F_0 is the fluorescence in the absence of halide F is the fluorescence in the presence of halide K is the Stern–Volmer quenching constant, which depends on the chloride concentration, l^-/math>. in a linear manner. Thus, quinoline-based indicators are one-wavelength dyes - the signal results from monitoring the fluorescence at a single wavelength. Ratiometric measurement of halide concentration is not possible with quin ...
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Chemical Analysis
Analytical chemistry studies and uses instruments and methods to separate, identify, and quantify matter. In practice, separation, identification or quantification may constitute the entire analysis or be combined with another method. Separation isolates analytes. Qualitative analysis identifies analytes, while quantitative analysis determines the numerical amount or concentration. Analytical chemistry consists of classical, wet chemical methods and modern analytical techniques. Classical qualitative methods use separations such as precipitation, extraction, and distillation. Identification may be based on differences in color, odor, melting point, boiling point, solubility, radioactivity or reactivity. Classical quantitative analysis uses mass or volume changes to quantify amount. Instrumental methods may be used to separate samples using chromatography, electrophoresis or field flow fractionation. Then qualitative and quantitative analysis can be performed, often wi ...
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Formate
Formate (IUPAC name: methanoate) is the conjugate base of formic acid. Formate is an anion () or its derivatives such as ester of formic acid. The salts and esters are generally colorless. Fundamentals When dissolved in water, formic acid converts to formate: : Formate is a planar anion. The two oxygen atoms are equivalent and bear a partial negative charge. The remaining C-H bond is not acidic. Biochemistry : Formate is a common C-1 source in living systems. It is formed from many precursors including choline, serine, and sarcosine. It provides a C-1 source in the biosynthesis of some nucleic acids. Formate (or formic acid) is invoked as a leaving group in the demethylation of some sterols. These conversions are catalyzed by aromatase enzymes using O2 as the oxidant. Specific conversions include testosterone to estradiol and androstenedione to estrone. Formate is reversibly oxidized by the enzyme formate dehydrogenase from ''Desulfovibrio gigas'': : Formate esters ...
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Analytical Chemistry
Analytical skill, Analytical chemistry studies and uses instruments and methods to Separation process, separate, identify, and Quantification (science), quantify matter. In practice, separation, identification or quantification may constitute the entire analysis or be combined with another method. Separation isolates analytes. Qualitative inorganic analysis, Qualitative analysis identifies analytes, while Quantitative analysis (chemistry), quantitative analysis determines the numerical amount or concentration. Analytical chemistry consists of classical, wet chemistry, wet chemical methods and modern analytical techniques. Classical qualitative methods use separations such as Precipitation (chemistry), precipitation, Extraction (chemistry), extraction, and distillation. Identification may be based on differences in color, odor, melting point, boiling point, solubility, radioactivity or reactivity. Classical quantitative analysis uses mass or volume changes to quantify amount. Ins ...
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Journal Of Neuroscience Methods
The ''Journal of Neuroscience Methods'' is a peer-reviewed scientific journal covering scientific techniques and protocols used in any branch of neuroscience research. It was established in 1979. The editor-in-chief is Giuseppe Di Giovanni, Professor of Neuroscience at the University of Malta and Honorary Professor at Cardiff University, UK. Journal Neuroscience Methods is published 18 times a year by Elsevier. Associate Editors: Vince Calhoun ( The Mind Research Network, Albuquerque, New Mexico, USA), Jing Jin (East China University of Science and Technology, Shanghai, China), Philippe De Deurwaerdère (Université de Bordeaux, Bordeaux Cedex, France), Robert Hampson (Wake Forest University Health Sciences, Winston-Salem, North Carolina, USA), Liset Menendez de la Prida ( Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain), Patrizia Campolongo (University of Rome La Sapienza, Rome, Italy), Floris Wouterlood (Vrije Universiteit Amsterdam, Amsterdam, Neth ...
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Neuron (journal)
''Neuron'' is a biweekly peer-reviewed scientific journal published by Cell Press, an imprint of Elsevier. Established in 1988, it covers neuroscience and related biological processes. The current editor-in-chief An editor-in-chief (EIC), also known as lead editor or chief editor, is a publication's editorial leader who has final responsibility for its operations and policies. The editor-in-chief heads all departments of the organization and is held accoun ... is Mariela Zirlinger. The founding editors were Lily Jan, A. James Hudspeth, Louis Reichardt, Roger Nicoll, and Zach Hall. A past editor-in-chief was Katja Brose. Transcript and video available. Click on "Transcript" for text. * See alsoA Career in Science Editing: Katja BroseEditor in Chief, Neuron References Neuroscience journals Cell Press academic journals Academic journals established in 1988 English-language journals Biweekly journals {{neuroscience-journal-stub ...
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Journal Of Biological Chemistry
The ''Journal of Biological Chemistry'' (''JBC'') is a weekly peer-reviewed scientific journal that was established in 1905., jbc.org Since 1925, it is published by the American Society for Biochemistry and Molecular Biology. It covers research in areas of biochemistry and molecular biology. The editor is Alex Toker. the journal is fully open access. In press articles are available free on its website immediately after acceptance. Editors The following individuals have served as editors of the journal: * 1906–1909: John Jacob Abel and Christian Archibald Herter * 1909–1910: Christian Archibald Herter * 1910–1914: Alfred Newton Richards * 1914–1925: Donald D. Van Slyke * 1925–1936: Stanley R. Benedict. After Benedict died, John T. Edsall served as temporary editor until the next editor was appointed. * 1937–1958: Rudolph J. Anderson * 1958–1967: John T. Edsall * 1968–1971: William Howard Stein * 1971–2011: Herbert Tabor * 2011–2015: Martha Fedor * ...
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Current Biology
''Current Biology'' is a biweekly peer-reviewed scientific journal that covers all areas of biology, especially molecular biology, cell biology, genetics, neurobiology, ecology, and evolutionary biology. The journal includes research articles, various types of review articles, as well as an editorial magazine section. The journal was established in 1991 by the Current Science group, was acquired by Elsevier in 1998, and has since 2001 been part of Cell Press, a subdivision of Elsevier. According to '' Journal Citation Reports'', the journal has a 2020 impact factor of 10.834. It was categorized as a "high impact journal" by the Superfund Research Program. References External links * Biology journals English-language journals Cell Press academic journals Academic journals established in 1991 Biweekly journals {{biology-journal-stub ...
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American Journal Of Physiology
The ''American Journal of Physiology'' is a peer-reviewed scientific journal on physiology published by the American Physiological Society. Vols. for 1898–1941 and 1948-56 include the Society's proceedings, including abstracts of papers presented at the 10th-53rd annual meetings, and the 1948-56 fall meetings. Subjournals The ''American Journal of Physiology'' has seven subjournals; according to the 2019 Journal Citation Reports their impact factor The impact factor (IF) or journal impact factor (JIF) of an academic journal is a type of journal ranking. Journals with higher impact factor values are considered more prestigious or important within their field. The Impact Factor of a journa ...s vary from 2.992 to 4.406: * ''AJP-Cell Physiology'' * ''AJP-Endocrinology and Metabolism'' * ''AJP-Gastrointestinal and Liver Physiology'' * ''AJP-Heart and Circulatory Physiology'' * ''AJP-Lung Cellular and Molecular Physiology'' * ''AJP-Regulatory, Integrative and Comparative Phy ...
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Polypeptide
Peptides are short chains of amino acids linked by peptide bonds. A polypeptide is a longer, continuous, unbranched peptide chain. Polypeptides that have a molecular mass of 10,000 Da or more are called proteins. Chains of fewer than twenty amino acids are called oligopeptides, and include dipeptides, tripeptides, and tetrapeptides. Peptides fall under the broad chemical classes of biological polymers and oligomers, alongside nucleic acids, oligosaccharides, polysaccharides, and others. Proteins consist of one or more polypeptides arranged in a biologically functional way, often bound to ligands such as coenzymes and cofactors, to another protein or other macromolecule such as DNA or RNA, or to complex macromolecular assemblies. Amino acids that have been incorporated into peptides are termed residues. A water molecule is released during formation of each amide bond.. All peptides except cyclic peptides have an N-terminal (amine group) and C-terminal (ca ...
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Cyan Fluorescent Protein
The green fluorescent protein (GFP) is a protein that exhibits green fluorescence when exposed to light in the blue to ultraviolet range. The label ''GFP'' traditionally refers to the protein first isolated from the jellyfish ''Aequorea victoria'' and is sometimes called ''avGFP''. However, GFPs have been found in other organisms including corals, sea anemones, zoanithids, copepods and lancelets. The GFP from ''A. victoria'' has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. The fluorescence quantum yield (QY) of GFP is 0.79. The GFP from the sea pansy ('' Renilla reniformis'') has a single major excitation peak at 498 nm. GFP makes for an excellent tool in many forms of biology due to its ability to form an internal chromophore without requiring any accessory cofactors, gene products, or enzymes / substrates other than molecular oxy ...
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Förster Resonance Energy Transfer
Förster resonance energy transfer (FRET), fluorescence resonance energy transfer, resonance energy transfer (RET) or electronic energy transfer (EET) is a mechanism describing energy transfer between two light-sensitive molecules (chromophores). A donor chromophore, initially in its electronic excited state, may transfer energy to an acceptor chromophore through nonradiative dipole–dipole coupling. The efficiency of this energy transfer is inversely proportional to the sixth power of the distance between donor and acceptor, making FRET extremely sensitive to small changes in distance. Measurements of FRET efficiency can be used to determine if two fluorophores are within a certain distance of each other. Such measurements are used as a research tool in fields including biology and chemistry. FRET is analogous to Near and far field, near-field communication, in that the radius of interaction is much smaller than the Light, wavelength of light emitted. In the near-field region, t ...
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Ionization
Ionization or ionisation is the process by which an atom or a molecule acquires a negative or positive Electric charge, charge by gaining or losing electrons, often in conjunction with other chemical changes. The resulting electrically charged atom or molecule is called an ion. Ionization can result from the loss of an electron after collisions with subatomic particles, collisions with other atoms, molecules, electrons, positrons, protons, antiprotons, and ions, or through the interaction with electromagnetic radiation. Heterolytic bond cleavage and heterolytic substitution reactions can result in the formation of ion pairs. Ionization can occur through radioactive decay by the internal conversion process, in which an excited nucleus transfers its energy to one of the inner-shell electrons causing it to be ejected. Uses Everyday examples of gas ionization occur within a fluorescent lamp or other electrical discharge lamps. It is also used in radiation detectors such as the Geiger- ...
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