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Desiccation
Desiccation is the state of extreme dryness, or the process of extreme drying. A desiccant is a hygroscopic (attracts and holds water) substance that induces or sustains such a state in its local vicinity in a moderately sealed container. The word desiccation comes . Industry Desiccation is widely employed in the oil and gas industry. These materials are obtained in a hydrated state, but the water content leads to corrosion or is incompatible with downstream processing. Removal of water is achieved by cryogenics, cryogenic condensation, absorption into glycols, and absorption onto desiccants such as silica gel. Laboratory A desiccator is a heavy glass or plastic container, now somewhat antiquated, used in practical chemistry for drying or keeping small amounts of materials very dry. The material is placed on a shelf, and a drying agent or ''desiccant'', such as dry silica gel or anhydrous sodium hydroxide, is placed below the shelf. Often some sort of humidity indicator is ...
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Deinococcus Radiodurans
''Deinococcus radiodurans'' is a bacterium, an extremophile and one of the most radiation-resistant organisms known. It can survive cold, dehydration, vacuum, and acid, and therefore is known as a polyextremophile. ''The Guinness Book Of World Records'' listed it in January 1998 as the world's most radiation-resistant bacterium or lifeform. Several bacteria of comparable radioresistance are known, including some species of the genus '' Chroococcidiopsis'' (phylum cyanobacteria) and some species of '' Rubrobacter'' (phylum Actinomycetota); among the archaea, the species '' Thermococcus gammatolerans'' shows comparable radioresistance. Name and classification The name ''Deinococcus radiodurans'' derives from the Ancient Greek δεινός () and κόκκος () meaning "terrible grain/berry" and the Latin and , meaning "radiation-surviving". The species was formerly called ''Micrococcus radiodurans''. As a consequence of its hardiness, it has been nicknamed “Conan the Bacteri ...
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Hygroscopic
Hygroscopy is the phenomenon of attracting and holding water molecules via either absorption (chemistry), absorption or adsorption from the surrounding Natural environment, environment, which is usually at normal or room temperature. If water molecules become suspended among the substance's molecules, adsorbing substances can become physically changed, e.g. changing in volume, boiling point, viscosity or some other physical characteristic or property of the substance. For example, a finely dispersed hygroscopic powder, such as a salt, may become clumpy over time due to collection of moisture from the surrounding environment. ''Deliquescent'' materials are sufficiently hygroscopic that they dissolve in the water they absorb, forming an aqueous solution. Hygroscopy is essential for many plant and animal species' attainment of hydration, nutrition, reproduction and/or seed dispersal. Biological evolution created hygroscopic solutions for water harvesting, filament tensile strength, ...
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Non-homologous End Joining
Non-homologous end joining (NHEJ) is a pathway that repairs double-strand breaks in DNA. It is called "non-homologous" because the break ends are directly ligated without the need for a homologous template, in contrast to homology directed repair (HDR), which requires a homologous sequence to guide repair. NHEJ is active in both non-dividing and proliferating cells, while HDR is not readily accessible in non-dividing cells. The term "non-homologous end joining" was coined in 1996 by Moore and Haber. NHEJ is typically guided by short homologous DNA sequences called microhomologies. These microhomologies are often present in single-stranded overhangs on the ends of double-strand breaks. When the overhangs are perfectly compatible, NHEJ usually repairs the break accurately. Imprecise repair leading to loss of nucleotides can also occur, but is much more common when the overhangs are not compatible. Inappropriate NHEJ can lead to translocations and telomere fusion, hallmarks of t ...
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Mycobacterium Smegmatis
''Mycobacterium smegmatis'' is an acid-fast bacterium, bacterial species in the phylum ''Actinomycetota'' and the genus ''Mycobacterium''. It is 3.0 to 5.0 μm long with a bacillus (shape), bacillus shape and can be stained by Ziehl–Neelsen method and the auramine-rhodamine fluorescent method. It was first reported in November 1884, who found a bacillus with the staining appearance of tubercle bacilli in syphilis, syphilitic chancres. Subsequent to this, Alvarez and Tavel found organisms similar to that described by Lustgarten also in normal genital secretions (smegma). This organism was later named ''M. smegmatis''. Some species of the genus ''Mycobacterium'' have recently been renamed to ''Mycolicibacterium'', so that ''M. smegmatis'' is now ''Mycolicibacterium smegmatis''. ''M. smegmatis'', which was previously considered a nonmotile organism, uses a sliding mechanism that allows it to move around its environment. Henrichsen defines it as, “a kind of surface translo ...
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Desiccant
A desiccant is a hygroscopic substance that is used to induce or sustain a state of dryness (desiccation) in its vicinity; it is the opposite of a humectant. Commonly encountered pre-packaged desiccants are solids that absorb water. Desiccants for specialized purposes may be in forms other than solid, and may work through other principles, such as chemical bonding of water molecules. They are commonly encountered in foods to retain crispness. Industrially, desiccants are widely used to control the level of water in gas streams. Types of desiccants Although some desiccants are chemically inert, others are extremely reactive and require specialized handling techniques. The most common desiccant is silica gel, an otherwise inert, nontoxic, water-insoluble white solid. Tens of thousands of tons are produced annually for this purpose. Other common desiccants include activated charcoal, calcium sulfate, calcium chloride, and molecular sieves (typically, zeolites). Desicc ...
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Dauer Larva
Dauer ( German "", English "the endurance", "persistence"; "unlimited time" ) describes an alternative developmental stage of nematode worms, particularly rhabditids including ''Caenorhabditis elegans'', whereby the larva goes into a type of stasis and can survive harsh conditions. Since the entrance of the dauer stage is dependent on environmental cues, it represents a classic and well studied example of polyphenism. The dauer state is given other names in the various types of nematodes such as ‘ diapause’ or ‘hypobiosis’, but since the ''C. elegans'' nematode has become the most studied nematode, the term ‘dauer stage’ or 'dauer larvae' is becoming universally recognised when referring to this state in other free-living nematodes. The dauer stage is also considered to be equivalent to the infective stage of parasitic nematode larvae. As Émile Maupas first proposed in 1899 and 1900, all nematodes have five stages separated by four moults. Under environmental condit ...
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Caenorhabditis Elegans
''Caenorhabditis elegans'' () is a free-living transparent nematode about 1 mm in length that lives in temperate soil environments. It is the type species of its genus. The name is a Hybrid word, blend of the Greek ''caeno-'' (recent), ''rhabditis'' (rod-like) and Latin ''elegans'' (elegant). In 1900, Émile Maupas, Maupas initially named it ''Rhabditidae, Rhabditides elegans.'' Günther Osche, Osche placed it in the subgenus ''Caenorhabditis'' in 1952, and in 1955, Ellsworth Dougherty, Dougherty raised ''Caenorhabditis'' to the status of genus. ''C. elegans'' is an unsegmented pseudocoelomate and lacks respiratory or circulatory systems. Most of these nematodes are hermaphrodites and a few are males. Males have specialised tails for mating that include spicule (nematode), spicules. In 1963, Sydney Brenner proposed research into ''C. elegans,'' primarily in the area of neuronal development. In 1974, he began research into the molecular biology, molecular and developmental ...
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Slug
Slug, or land slug, is a common name for any apparently shell-less Terrestrial mollusc, terrestrial gastropod mollusc. The word ''slug'' is also often used as part of the common name of any gastropod mollusc that has no shell, a very reduced shell, or only a small internal shell, particularly sea slugs and semi-slugs (this is in contrast to the common name ''snail'', which applies to gastropods that have a coiled shell large enough that they can fully retract their soft parts into it). Various Taxonomy (biology), taxonomic families of land slugs form part of several quite different evolutionary lineages, which also include snails. Thus, the various families of slugs are not closely related, despite the superficial similarity in overall body form. The shell-less condition has arisen many times independently as an example of convergent evolution, and thus the category "slug" is Polyphyly, polyphyletic. Taxonomy Of the six orders of Pulmonata, two – the Onchidiacea and Soleoli ...
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Bacillus Subtilis
''Bacillus subtilis'' (), known also as the hay bacillus or grass bacillus, is a gram-positive, catalase-positive bacterium, found in soil and the gastrointestinal tract of ruminants, humans and marine sponges. As a member of the genus ''Bacillus'', ''B. subtilis'' is rod-shaped, and can form a tough, protective endospore, allowing it to tolerate extreme environmental conditions. ''B. subtilis'' has historically been classified as an obligate aerobe, though evidence exists that it is a facultative anaerobe. ''B. subtilis'' is considered the best studied Gram-positive bacterium and a model organism to study bacterial chromosome replication and cell differentiation. It is one of the bacterial champions in secreted enzyme production and used on an industrial scale by biotechnology companies. Description ''Bacillus subtilis'' is a Gram-positive bacterium, rod-shaped and catalase-positive. It was originally named ''Vibrio subtilis'' by Christian Gottfried Ehrenberg, an ...
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Desiccator
Desiccators are sealable enclosures containing desiccants used for preserving moisture-sensitive items such as cobalt chloride paper for another use. A common use for desiccators is to protect chemicals which are hygroscopic or which react with water from humidity. The contents of desiccators are exposed to atmospheric moisture whenever the desiccators are opened. It also requires some time to achieve a low humidity. Hence they are not appropriate for storing chemicals which react quickly or violently with atmospheric moisture such as the alkali metals; a glovebox or Schlenk-type apparatus may be more suitable for these purposes. Desiccators are sometimes used to remove traces of water from an almost-dry sample. Where a desiccator alone is unsatisfactory, the sample may be dried at elevated temperature using Abderhalden's drying pistol. Constituents The lower compartment of the desiccator contains lumps of silica gel, freshly calcined quicklime, Drierite, molecular s ...
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Endospore
An endospore is a dormant, tough, and non-reproductive structure produced by some bacteria in the phylum Bacillota. The name "endospore" is suggestive of a spore or seed-like form (''endo'' means 'within'), but it is not a true spore (i.e., not an offspring). It is a stripped-down, dormant form to which the bacterium can reduce itself. Endospore formation is usually triggered by a lack of nutrients, and usually occurs in Gram-positive bacteria. In endospore formation, the bacterium divides within its cell wall, and one side then engulfs the other. Endospores enable bacteria to lie dormant for extended periods, even centuries. There are many reports of spores remaining viable over 10,000 years, and revival of spores millions of years old has been claimed. There is one report of viable spores of '' Bacillus marismortui'' in salt crystals approximately 25 million years old. When the environment becomes more favorable, the endospore can reactivate itself into a vegetative state. Mos ...
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Ecology
Ecology () is the natural science of the relationships among living organisms and their Natural environment, environment. Ecology considers organisms at the individual, population, community (ecology), community, ecosystem, and biosphere levels. Ecology overlaps with the closely related sciences of biogeography, evolutionary biology, genetics, ethology, and natural history. Ecology is a branch of biology, and is the study of abundance (ecology), abundance, biomass (ecology), biomass, and distribution of organisms in the context of the environment. It encompasses life processes, interactions, and adaptations; movement of materials and energy through living communities; ecological succession, successional development of ecosystems; cooperation, competition, and predation within and between species; and patterns of biodiversity and its effect on ecosystem processes. Ecology has practical applications in fields such as conservation biology, wetland management, natural resource m ...
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