|
CyTOF
Cytometry by time of flight, or CyTOF, is an application of mass cytometry used to quantify labeled targets on the surface and interior of single cells. CyTOF allows the quantification of multiple cellular components simultaneously using an Inductively coupled plasma mass spectrometry, ICP-MS detector. CyTOF takes advantage of immunolabeling to quantify proteins, carbohydrates or lipids in a cell. Targets are selected to answer a specific research question and are labeled with lanthanide metal tagged antibodies. Labeled cells are nebulized and mixed with heated argon gas to dry the cell containing particles. The sample-gas mixture is focused and ignited with an argon plasma torch. This breaks the cells into their individual atoms and creates an ion cloud. Abundant low weight ions generated from environmental air and biological molecules are removed using a quadrupole mass analyzer. The remaining heavy ions from the antibody tags are quantified by Time-of-flight mass spectrometry ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mass Cytometry
Mass cytometry is a mass spectrometry technique based on inductively coupled plasma mass spectrometry and time of flight mass spectrometry used for the determination of the properties of cells (cytometry). In this approach, antibodies are Conjugated protein, conjugated with Isotope, isotopically pure elements, and these antibodies are used to label cellular proteins. Cells are nebulized and sent through an argon Plasma (physics), plasma, which ionizes the metal-conjugated antibodies. The metal signals are then analyzed by a time-of-flight mass spectrometer. The approach overcomes limitations of spectral overlap in flow cytometry by utilizing discrete isotopes as a reporter system instead of traditional fluorophores which have broad emission spectra. Commercialization Tagging technology and instrument development occurred at the University of Toronto and DVS Sciences, Inc. CyTOF (cytometry by time of flight) was initially commercialized by DVS Sciences in 2009. In 2014, Fluidigm acq ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Olga Ornatsky
Olga Ornatsky is a Soviet born, Canadian scientist. Ornatsky co-founded DVS Sciences in 2004 (acquired by Fluidigm in 2014 and then renamed to Standard BioTools in 2022) along with Dmitry Bandura, Vladimir Baranov and Scott D. Tanner. Biography Ornatsky graduated from the Moscow State University, Department of Biology. In 1989, she completed her Ph.D. in cell and molecular biology, and worked as a research scientist at the Cardiology Centre studying vascular smooth muscle involvement in atherosclerosis. In 1993, she immigrated to Canada, and became a postdoctoral research fellow at York University. She quickly progressed to become senior research associate in the Laboratory of Vascular Biology and Cardiac Surgery at St. Michael's Hospital (Toronto). Her achievements brought her to MDS Proteomics Inc. (noProtana Inc, where she led her research group as a senior scientist for four years. In 2005, she left MDS to pursue a different direction. Together with the co-founders of ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Vladimir Baranov
Vladimir Baranov is a Soviet born Canadian scientist and one of the original co-inventors of Mass cytometry technology... He co-founded DVS Sciences in 2004 (acquired by Fluidigm in 2014 and then renamed to Standard BioTools in 2022) along with Dmitry Bandura, Scott D. Tanner and Olga Ornatsky. Biography In 1993, he immigrated to Canada. Prior to the formation of DVS Sciences. Dr. Baranov, a senior scientist at MDS SCIEX, was a key member of the research team that developed and promoted the Dynamic Reaction Cell®, which remains today at the pinnacle of quadrupole ICP-MS technology. In 2005, together with Scott D. Tanner and Dmitry Bandura, he began independently developing an ICP-TOF-MS based cytometer and became a researcher at the University of Toronto in March 2005. After securing ample funding by 2010 from various sources, including National Institutes of Health, Ontario Institute for Cancer Research (OICR), the Ministry of Research and Innovation, Ontario Centres of ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Dmitry Bandura
Dmitry Bandura is a Soviet-born Canadian scientist, notable for being one of the co-inventors of the Mass cytometry technology. Bandura co-founded DVS Sciences in 2004 (acquired by Fluidigm in 2014 and then renamed to Standard BioTools in 2022) along with Drs Vladimir Baranov, Scott D. Tanner, and Olga Ornatsky. Biography Bandura grew up in Chernivtsi, Ukraine, where he graduated from school #35 with distinction. He received an MSc in engineering physics in 1985 and a PhD in technical sciences, both supervised by ProfessoAlexander A. Sysoevref name="bio"> at Moscow Engineering Physics Institute. His PhD thesis research focused on elemental analysis of hypervelocity microparticles via time-of-flight mass spectrometry (TOF-MS) of their impact-induced plasma. Bandura emigrated to Australia in 1992, where he worked as a Research Physicist aGBC Scientific Equipment There, he worked on the development of inductively coupled plasma mass spectrometry (ICP-MS), contributing to the re ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
