|
Arthrobacter Luteus
''Arthrobacter luteus'' (ALU) is a species of gram-positive bacteria in the genus ''Arthrobacter''. ''A. luteus'' is facultatively anaerobic, Pleomorphism (microbiology), pleomorphic, branching, Motility, non-motile, Spore, non-sporulating, non-acid-fast, catalase-positive, and Bacillus (shape), rod-shaped (0.6–1.0 μm × 0.8–10.0 μm). A Restriction enzyme, restriction endonuclease enzyme is extracted from the bacterium and acts at the centre of a Palindromic sequence, palindromic tetranucleotide sequence to give even-ended Duplex sequencing, duplex DNA fragmentation, DNA fragments phosphorylated at the 5'-end. The restriction site ''Alu-I'' itself is a 4-base cutter: AG/CT. The Alu element, Alu retrotransposon is named after the bacterium's abbreviation. The bacterium is also used to produce zymolyase, which can degrade yeast cell wall. Background ''Arthrobacter luteus'' was isolated from brewery sewage in research done in Takasaki, Japan in 1969. The team studied the ba ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gram-positive Bacteria
In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. The Gram stain is used by microbiologists to place bacteria into two main categories, gram-positive (+) and gram-negative (−). Gram-positive bacteria have a thick layer of peptidoglycan within the cell wall, and gram-negative bacteria have a thin layer of peptidoglycan. Gram-positive bacteria retain the crystal violet stain used in the test, resulting in a purple color when observed through an optical microscope. The thick layer of peptidoglycan in the bacterial cell wall retains the stain after it has been fixed in place by iodine. During the decolorization step, the decolorizer removes crystal violet from all other cells. Conversely, gram-negative bacteria cannot retain the violet stain after the decolorization step; alcohol used in this stage ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Tetranucleotide
Nucleotides are organic molecules composed of a nitrogenous base, a pentose sugar and a phosphate. They serve as monomeric units of the nucleic acid polymers – deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), both of which are essential biomolecules within all life-forms on Earth. Nucleotides are obtained in the diet and are also synthesized from common nutrients by the liver. Nucleotides are composed of three subunit molecules: a nucleobase, a five-carbon sugar (ribose or deoxyribose), and a phosphate group consisting of one to three phosphates. The four nucleobases in DNA are guanine, adenine, cytosine, and thymine; in RNA, uracil is used in place of thymine. Nucleotides also play a central role in metabolism at a fundamental, cellular level. They provide chemical energy—in the form of the nucleoside triphosphates, adenosine triphosphate (ATP), guanosine triphosphate (GTP), cytidine triphosphate (CTP), and uridine triphosphate (UTP)—throughout the cell for t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
SV40
SV40 is an abbreviation for simian vacuolating virus 40 or simian virus 40, a polyomavirus that is found in both monkeys and humans. Like other polyomaviruses, SV40 is a DNA virus that is found to cause tumors in humans and animals, but most often persists as a dormant infection. SV40 has been widely studied as a model eukaryotic virus, leading to many early discoveries in eukaryotic DNA replication and transcription. Following contamination of polio vaccine batches in the 1950s and 1960s, SV40 came under suspicion as a possible cancer risk, but no subsequent increased cancer rate was observed, making such a risk unlikely. Nevertheless SV40 has become a ''cause célèbre'' for anti-vaccination activists, who have blamed it for multiple ills, including cancer and HIV/AIDS. Human disease The hypothesis that SV40 might cause cancer in humans was a particularly controversial area of research, fuelled by the historical contamination of some batches of polio vaccine with SV40 in the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Zymolyase
Zymolyase (also known as lyticase, sometimes misspelled as Zymolase) is an enzyme mixture used to degrade the cell wall of yeast and form spheroplasts. Essential activities of zymolyase include β-1,3-glucan laminaripentao-hydrolase activity and β-1,3-glucanase activity. A common source of zymolyase is the Actinobacteria ''Arthrobacter luteus''. Commercial sources of zymolyase may have some residual protease activity. Fungi susceptible to zymolyase can be found in the order Saccharomycetales. Saccharomycetales genera that zymolyase is known to have activity against include: '' Eremothecium'', ''Candida'', ''Debaryomyces'', '' Eremothecium'', '' Endomyces'', '' Hansenula'', '' Hanseniaspora'', ''Kloekera'', ''Kluyveromyces'', '' Lipomyces'', ''Metschikowia'', ''Pichia'', ''Pullularia'', ''Saccharomyces ''Saccharomyces'' is a genus of fungi that includes many species of yeasts. ''Saccharomyces'' is from Greek σάκχαρον (sugar) and μύκης (fungus) and means ''sugar ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Retrotransposon
Retrotransposons (also called Class I transposable elements) are mobile elements which move in the host genome by converting their transcribed RNA into DNA through reverse transcription. Thus, they differ from Class II transposable elements, or DNA transposons, in utilizing an RNA intermediate for the transposition and leaving the transposition donor site unchanged. Through reverse transcription, retrotransposons amplify themselves quickly to become abundant in eukaryotic genomes such as maize (49–78%) and humans (42%). They are only present in eukaryotes but share features with retroviruses such as HIV, for example, discontinuous reverse transcriptase-mediated extrachromosomal recombination. There are two main types of retrotransposons, long terminal repeats (LTRs) and non-long terminal repeats (non-LTRs). Retrotransposons are classified based on sequence and method of transposition. Most retrotransposons in the maize genome are LTR, whereas in humans they are mostly non-L ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Alu Element
An Alu element is a short stretch of DNA originally characterized by the action of the ''Arthrobacter luteus (Alu)'' restriction endonuclease. ''Alu'' elements are the most abundant transposable elements in the human genome, present in excess of one million copies. Most ''Alu'' elements are thought to be selfish or parasitic DNA. However, it has been suggested that at least some are likely to play a role in evolution and have been used as genetic markers. They are derived from the small cytoplasmic 7SL RNA, a component of the signal recognition particle. ''Alu'' elements are not highly conserved within primate genomes, as only a minority have retained activity, and originated in the genome of an ancestor of Supraprimates. ''Alu'' insertions have been implicated in several inherited human diseases and in various forms of cancer. The study of Alu elements has also been important in elucidating human population genetics and the evolution of primates, including the evolution of ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
5'-end
Directionality, in molecular biology and biochemistry, is the end-to-end chemical orientation of a single strand of nucleic acid. In a single strand of DNA or RNA, the chemical convention of naming carbon atoms in the nucleotide pentose-sugar-ring means that there will be a 5′ end (usually pronounced "five-prime end"), which frequently contains a phosphate group attached to the 5′ carbon of the ribose ring, and a 3′ end (usually pronounced "three-prime end"), which typically is unmodified from the ribose -OH substituent. In a DNA double helix, the strands run in opposite directions to permit base pairing between them, which is essential for replication or transcription of the encoded information. Nucleic acids can only be synthesized in vivo in the 5′-to-3′ direction, as the polymerases that assemble various types of new strands generally rely on the energy produced by breaking nucleoside triphosphate bonds to attach new nucleoside monophosphates to the 3′- ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Phosphorylated
In biochemistry, phosphorylation is described as the "transfer of a phosphate group" from a donor to an acceptor. A common phosphorylating agent (phosphate donor) is ATP and a common family of acceptor are alcohols: : This equation can be written in several ways that are nearly equivalent that describe the behaviors of various protonated states of ATP, ADP, and the phosphorylated product. As is clear from the equation, a phosphate group per se is not transferred, but a phosphoryl group (PO3-). Phosphoryl is an electrophile. This process and its inverse, dephosphorylation, are common in biology. Text was copied from this source, which is available under a Creative Commons Attribution 4.0 International License. Protein phosphorylation often activates (or deactivates) many enzymes. During respiration Phosphorylation is essential to the processes of both anaerobic and aerobic respiration, which involve the production of adenosine triphosphate (ATP), the "high-energy" exchang ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
DNA Fragmentation
DNA fragmentation is the separation or breaking of DNA strands into pieces. It can be done intentionally by laboratory personnel or by cells, or can occur spontaneously. Spontaneous or accidental DNA fragmentation is fragmentation that gradually accumulates in a cell. It can be measured by e.g. the comet assay or by the TUNEL assay. Its main units of measurement is the DNA Fragmentation Index (DFI). A DFI of 20% or more significantly reduces the success rates after ICSI. DNA fragmentation was first documented by Williamson in 1970 when he observed discrete oligomeric fragments occurring during cell death in primary neonatal liver cultures. He described the cytoplasmic DNA isolated from mouse liver cells after culture as characterized by DNA fragments with a molecular weight consisting of multiples of 135 kDa. This finding was consistent with the hypothesis that these DNA fragments were a specific degradation product of nuclear DNA. Intentional DNA fragmentation is often neces ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Duplex Sequencing
Duplex sequencing is a Library (biology), library preparation and analysis method for DNA sequencing, next-generation sequencing (NGS) platforms that employs random tagging of double-stranded DNA to detect mutations with higher accuracy and lower error rates. This method uses Degenerate bases, degenerate molecular tags in addition to sequencing adapters to recognize reads originating from each strand of DNA. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors result in mutations in only one strand and can thus be discounted as technical error. Duplex sequencing theoretically can detect mutations with frequencies as low as 5 x 10−8 --that is more than 10,000 times higher in accuracy compared to the conventional next-generation sequencing methods.M. W. Schmitt, S. R. Kennedy, J. J. Salk, et al“Detection of ultra-rare mutations by next-generation sequencing” Proc. Natl. Acad. Sci., vol. 109 n ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Palindromic Sequence
A palindromic sequence is a nucleic acid sequence in a double-stranded DNA or RNA molecule whereby reading in a certain direction (e.g. 5' to 3') on one strand is identical to the sequence in the same direction (e.g. 5' to 3') on the complementary strand. This definition of palindrome thus depends on complementary strands being palindromic of each other. The meaning of palindrome in the context of genetics is slightly different from the definition used for words and sentences. Since a double helix is formed by two paired antiparallel strands of nucleotides that run in opposite directions, and the nucleotides always pair in the same way (adenine (A) with thymine (T) in DNA or uracil (U) in RNA; cytosine (C) with guanine (G)), a (single-stranded) nucleotide sequence is said to be a palindrome if it is equal to its reverse complement. For example, the DNA sequence ACCTAGGT is palindromic with its nucleotide-by-nucleotide complement TGGATCCA because reversing the order of the n ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Arthrobacter
''Arthrobacter'' (from the Greek, "jointed small stick”) is a genus of bacterium, bacteria that is commonly found in soil. All species in this genus are Gram-positive obligate aerobes that are bacterial shape, rods during exponential growth and bacterial shape, cocci in their Stationary phase (biology), stationary phase. ''Arthrobacter'' have a distinctive method of cell division called "snapping division" or reversion (microbiology), reversion in which the outer bacterial cell wall ruptures at a joint. Description ''Arthrobacter'' can be grown on mineral salts pyridone broth, where colonies have a greenish metallic center on incubated at . Under the microscope, ''Arthrobacter'' appear as rods when rapidly dividing, and cocci when in stationary phase. Dividing cells may also appear as chevrons ("V" shapes). Other notable characteristics are that it can use pyridone as its sole carbon source, and that its cocci are resistant to desiccation and starvation. Use in industry ''Arth ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
