The Seminole Gulf Railway (reporting mark SGLR) is a short line freight and passenger excursion railroad headquartered in Fort Myers, Florida, that operates two former CSX Transportation railroad lines in Southwest Florida. The company's Fort Myers Line, which was previously the southernmost segment of CSX's former Fort Myers Subdivision, runs from Arcadia south to North Naples via Punta Gorda, Fort Myers, Estero, and Bonita Springs. They also operate another former CSX line that runs from Oneco south through Sarasota. Seminole Gulf acquired the lines in November 1987 and operates its own equipment.
Seminole Gulf Railway is one of two freight railroad operating in Southwest Florida (the other is South Central Florida Express, who operates tracks farther inland near Clewiston). Freight transported by Seminole Gulf Railway includes lumber, propane, stone, steel, scrap metal, and other commodities. Freight is interchanged with CSX usually once or twice a week on each line. On the Fort Myers Line, freight trains between the yard in Fort Myers and Arcadia are known as the Desoto Turn. The Desoto Turn switches customers between North Fort Myers and Arcadia while industries in Fort Myers and south of there are switched locally from the yard.
In addition to carrying freight, Seminole Gulf Railway also operates a popular Murder Mystery Dinner Train from Fort Myers. The dinner train runs five nights a week, year-round from a station at Colonial Boulevard north to a point just south of Punta Gorda before returning. The dinner train utilizes a fleet of 1930s-era vintage rail cars named after nearby barrier islands (some of which were previously Ringling Bros. and Barnum & Bailey Circus rail cars). The dinner train includes a five-course dinner and has featured over 80 different murder mystery productions throughout its history.
Special holiday dinner trains also operate including Sweetheart Express on Valentine's Day, Halloween Ghost Train, New Year's Eve Gala, and Christmas Rail-Boat (which runs to the historic Punta Gorda Atlantic Coast Line Depot for a Christmas boat tour through Punta Gorda Isles) to name a few.
Seminole Gulf has also operated general excursion trains in the past. Seminole Gulf introduced passenger excursion trains in 1991 after losing a major freight customer. Excursion trains and were initially based from a small platform in North Naples near Railhead Park, but moved to the current station at Colonial Boulevard in Fort Myers shortly after.
The dinner and excursion trains are often pulled by one of two vintage EMD F-unit locomotives, SGLR 501 and SGLR 502. SGLR 501, an F9AM, was previously operated by the Milwaukee Road, while SGLR 502, an F7, was previously operated on the Baltimore and Ohio Railroad. Both were later acquired by the Long Island Rail Road, where they were converted into cab cars and used from the 1970s until 1999, before being sent to the SGLR.