A micromanipulator is a device which is used to physically interact with a sample under a microscope, where a level of precision of movement is necessary that cannot be achieved by the unaided human hand. It may typically consist of an input joystick, a mechanism for reducing the range of movement and an output section with the means of holding a microtool to hold, inject, cut or otherwise manipulate the object as required. The mechanism for reducing the movement usually requires the movement to be free of

backlash Backlash may refer to: Literature * '' Backlash: The Undeclared War Against American Women'', a 1991 book by Susan Faludi * ''Backlash'' (Star Wars novel), a 2010 novel by Aaron Allston * Backlash (Marc Slayton), comic book character * ''Backla ...

. This is achieved by the use of

kinematic Kinematics is a subfield of physics, developed in classical mechanics, that describes the motion of points, bodies (objects), and systems of bodies (groups of objects) without considering the forces that cause them to move. Kinematics, as a fiel ...

constraints to allow each part of the mechanism to move only in one or more chosen degrees of freedom, which achieves a high precision and repeatability of movement, usually at the expense of some absolute accuracy.

Movement

Movement reduction can be performed by mechanical levers, hydraulically using pistons of different diameters connected by tubing containing non-compressible fluid, electronically using stepper motors or linear actuators, or combinations of techniques in one instrument. Mechanisms with different ranges of movement or variable reduction ratio may be incorporated in one instrument to allow coarse and fine positioning. Depending on the application, users may require different scales of movement resolution, movement speed, range and accuracy. These are the critical variables integrated into manipulator design by manufacturers, which are typically presented to suit particular applications.

Microscope integration

Micromanipulators are usually used in conjunction with microscopes. Depending on the application, one or more micromanipulators may be fitted to a microscope stage or rigidly mounted to a bench next to a microscope. A typical application of micromanipulation is human

intracytoplasmic sperm injection Intracytoplasmic sperm injection (ICSI ) is an in vitro fertilization (IVF) procedure in which a single sperm cell is injected directly into the cytoplasm of an egg. This technique is used in order to prepare the gametes for the obtention of em ...

. Here, a spermatozoon measuring some 3 to 5 micrometres across is injected into an oocyte of approximately 100 micrometres in diameter, under the direct manual control of an embryologist. A disposable glass micropipette is fitted to a toolholder mounted on the output of the manipulator. The toolholder can be adjusted for different sized tools as well as the angle at which the tool is held.

Microelectronics

Micromanipulators are also used in applications such as microelectronics to position test probes onto small to medium scale

integrated circuits An integrated circuit or monolithic integrated circuit (also referred to as an IC, a chip, or a microchip) is a set of electronic circuits on one small flat piece (or "chip") of semiconductor material, usually silicon. Large numbers of tiny ...

and hybrid devices, and

patch clamp The patch clamp technique is a laboratory technique in electrophysiology used to study ionic currents in individual isolated living cells, tissue sections, or patches of cell membrane. The technique is especially useful in the study of excita ...

experiments in biological research.

Movement

Microscope integration

Microelectronics

References

Further reading