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Pipette
A pipette (sometimes spelled as pipett) is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more complex adjustable or electronic pipettes. Many pipette types work by creating a partial vacuum above the liquid-holding chamber and selectively releasing this vacuum to draw up and dispense liquid. Measurement accuracy varies greatly depending on the instrument. History The first simple pipettes were made in glass, such as Pasteur pipettes. Large pipettes continue to be made in glass; others are made in squeezable plastic for situations where an exact volume is not required. The first micropipette was patented in 1957 by Dr Heinrich Schnitger (Marburg, Germany). The founder of the company Eppendorf, Dr. Heinrich Netheler, inherited the rights and started t ...
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Pipettes
A pipette (sometimes spelled as pipett) is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more complex adjustable or electronic pipettes. Many pipette types work by creating a partial vacuum above the liquid-holding chamber and selectively releasing this vacuum to draw up and dispense liquid. Measurement accuracy varies greatly depending on the instrument. History The first simple pipettes were made in glass, such as Pasteur pipettes. Large pipettes continue to be made in glass; others are made in squeezable plastic for situations where an exact volume is not required. The first micropipette was patented in 1957 by Dr Heinrich Schnitger (Marburg, Germany). The founder of the company Eppendorf, Dr. Heinrich Netheler, inherited the rights and started th ...
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Pipette Tips-different 1
A pipette (sometimes spelled as pipett) is a laboratory tool commonly used in chemistry, biology and medicine to transport a measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more complex adjustable or electronic pipettes. Many pipette types work by creating a partial vacuum above the liquid-holding chamber and selectively releasing this vacuum to draw up and dispense liquid. Measurement accuracy varies greatly depending on the instrument. History The first simple pipettes were made in glass, such as Pasteur pipettes. Large pipettes continue to be made in glass; others are made in squeezable plastic for situations where an exact volume is not required. The first micropipette was patented in 1957 by Dr Heinrich Schnitger (Marburg, Germany). The founder of the company Eppendorf, Dr. Heinrich Netheler, inherited the rights and started th ...
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Graduated Pipettes
A graduated pipette is a pipette with its volume, in increments, marked along the tube. It is used to accurately measure and transfer a volume of liquid from one container to another. It is made from plastic or glass tubes and has a tapered tip. Along the body of the tube are graduation markings indicating volume from the tip to that point. A small pipette allows for more precise measurement of fluids; a larger pipette can be used to measure volumes when the accuracy of the measurement is less critical. Accordingly, pipettes vary in volume, with most measuring between . Types Mohr and Serological pipettes There are two types of pipettes that differ based on where the markings are located in reference to the pipette tip. These are Mohr pipettes and Serological pipettes, and they differ only by the position of the first graduation mark, nearest the tip of the pipette. A Mohr pipette is designed for use as a drain-out pipette. It has a straight tube and graduation marks indic ...
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Electronic Pipettes
Piston-driven air displacement pipettes are a type of micropipette, which are tools to handle volumes of liquid in the microliter scale. They are more commonly used in biology and biochemistry, and less commonly in chemistry; the equipment is susceptible to damage from many organic solvents. Operation These pipettes operate by piston-driven air displacement. A vacuum is generated by the vertical travel of a metallic or ceramic piston within an airtight sleeve. The upward movement of the piston, driven by the depression of the plunger, creates a vacuum in the space left vacant by the piston. To fill the vacuum, air from the tip rises, which is then replaced by the liquid that is drawn up into the tip and thus available for transport and dispensing elsewhere. Sterile technique prevents liquid from coming into contact with the pipette itself. Instead, the liquid is drawn into and dispensed from a disposable pipette tip that is discarded after transferring fluid and a new pipette ti ...
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Pasteur Pipette
An eye dropper, also called Pasteur pipette or simply dropper, is a device used to transfer small quantities of liquids. They are used in the laboratory and also to dispense small amounts of liquid medicines. A very common use was to dispense eye drops into the eye. The commonly recognized form is a glass tube tapered to a narrow point (a pipette) and fitted with a rubber bulb at the top, although many styles of both plastic and glass droppers exist. The combination of the pipette and rubber bulb has also been referred to as a teat pipette. The ''Pasteur pipette'' name is from the French scientist Louis Pasteur, who used a variant of them extensively during his research. In the past, there was no equipment to transfer a chemical solution without exposing it to the external environment. The hygiene and purity of chemical compounds is necessary for the expected result of each experiment. The eye dropper, both glass and plastic types, can be sterilized and plugged with a rubber bu ...
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Heinrich Schnitger
Heinrich Schnitger (10 May 1925 in Lemgo - 27 August 1964 in Upper Bavaria) was a German physician. He is considered the inventor of the piston stroke Micropipette, a laboratory device for dispensing small amounts of liquid. Life Heinrich Schnitger was born in 1925 in Lemgo, Westphalia, Germany, the son of an inventor who designed, among other things, a once widely used bicycle lock. As a young boy, Schnitger had already tinkered with and made many changes and improvements to various gadgets. After suffering from tuberculosis as a soldier in the Second World War, he decided to study medicine. He attained PhD after studying medicine in 1956 at the Philipps University of Marburg with a thesis on the development of a device for the automated determination of blood clotting times . During his subsequent work at the Institute of Physiological Chemistry University of Marburg, among other things, he worked on large amounts of chromatography samples with a volume of less than a milliliter ...
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Bulb Pipette
A volumetric pipette, bulb pipette, or belly pipette allows extremely accurate measurement (to four significant figures) of the volume of a solution. It is calibrated to deliver accurately a fixed volume of liquid. These pipettes have a large bulb with a long narrow portion above with a single graduation mark as it is calibrated for a single volume (like a volumetric flask). Typical volumes are 1, 2, 5, 10, 20, 25, 50 and 100 mL. Volumetric pipettes are commonly used in analytical chemistry to make laboratory solutions from a base stock as well as to prepare solutions for titration. ASTM standard E969 defines the standard tolerance for volumetric transfer pipettes. The tolerance depends on the size: a 0.5-mL pipette has a tolerance of ±0.006 mL, while a 50-mL pipette has a tolerance of ±0.05 mL. (These are for Class A pipettes; Class B pipettes are given a tolerance of twice that for the corresponding Class A.) A specialized example of a volumetric pipette is the microfluid ...
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Volumetric Pipettes
A volumetric pipette, bulb pipette, or belly pipette allows extremely accurate measurement (to four significant figures) of the volume of a solution. It is calibrated to deliver accurately a fixed volume of liquid. These pipettes have a large bulb with a long narrow portion above with a single graduation mark as it is calibrated for a single volume (like a volumetric flask). Typical volumes are 1, 2, 5, 10, 20, 25, 50 and 100 mL. Volumetric pipettes are commonly used in analytical chemistry to make laboratory solutions from a base stock as well as to prepare solutions for titration. ASTM standard E969 defines the standard tolerance for volumetric transfer pipettes. The tolerance depends on the size: a 0.5-mL pipette has a tolerance of ±0.006 mL, while a 50-mL pipette has a tolerance of ±0.05 mL. (These are for Class A pipettes; Class B pipettes are given a tolerance of twice that for the corresponding Class A.) A specialized example of a volumetric pipette is the microfluid ...
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Media Dispenser
A media dispenser or a culture media dispenser is a device for repeatedly delivering small fixed volumes (typically between 1 ml and 50 ml) of liquid such as a laboratory growth medium like molten agar or caustic or volatile solvents like toluene into a series of receptacles (Petri dishes, test tubes, Fernbach flasks, etc.). It is often important that such dispensers operate without biological or chemical contamination, and so must be internally sealed from the environment and designed for easy cleaning and sterilization before use. At a minimum, a media dispenser consists of some kind of pump connected to a length of discharge tubing or a spout. Dispensers used in laboratories are also frequently connected to microcontrollers to regulate the speed and volume of the medium as it leaves the pump. Laboratory media dispensers may include the following, among others: floating piston designs, syringe pumps, peristaltic pumps, pipettes or pipettors, and pressure injection ce ...
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Burette
A burette is a graduated glass tube with a tap at one end, for delivering known volumes of a liquid, especially in titrations. It is a long, graduated glass tube, with a stopcock at its lower end and a tapered capillary tube at the stopcock's outlet. The flow of liquid from the tube to the burette tip is controlled by the stopcock valve. There are two main types of burette; the volumetric burette and the piston burette. A volumetric burette delivers measured volumes of liquid. Piston burettes are similar to syringes, but with a precision bore and a plunger. Piston burettes may be manually operated or may be motorized. A weight burette delivers measured weights of a liquid. Overview A burette is a volumetric measuring glassware which is used in analytical chemistry for the accurate dispensing of a liquid, especially of one of the reagents in a titration. The burette tube carries graduated marks from which the dispensed volume of the liquid can be determined. Compared to a volumet ...
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Titration
Titration (also known as titrimetry and volumetric analysis) is a common laboratory method of quantitative chemical analysis to determine the concentration of an identified analyte (a substance to be analyzed). A reagent, termed the ''titrant'' or ''titrator'', is prepared as a standard solution of known concentration and volume. The titrant reacts with a solution of ''analyte'' (which may also be termed the ''titrand'') to determine the analyte's concentration. The volume of titrant that reacted with the analyte is termed the ''titration volume''. History and etymology The word "titration" descends from the French word ''titrer'' (1543), meaning the proportion of gold or silver in coins or in works of gold or silver; i.e., a measure of fineness or purity. ''Tiltre'' became ''titre'', which thus came to mean the "fineness of alloyed gold", and then the "concentration of a substance in a given sample". In 1828, the French chemist Joseph Louis Gay-Lussac first used ''titre'' as ...
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Eppendorf (company)
Eppendorf, a company with its registered office in Germany, develops, produces and sells products and services for laboratories around the world. Eppendorf products are used in academic and industrial research laboratories, e.g. in companies in the pharmaceutical, biotech, chemical and food industries. They are also used in laboratories that perform clinical or environmental analysis, in forensic laboratories, and in industrial laboratories where industrial process analysis, production and quality assurance are performed. Eppendorf describes its business as consisting of three divisions: liquid handling, cell handling, and sample handling. Products Eppendorf develops, produces and sells devices, consumables and services for laboratories. The liquid handling line includes products such as manual and electronic micropipettes, automated pipetting systems, and milliliter pipette controllers. The cell handling line includes products such as fermenters and bioreactors and cell culture ...
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