YEPD or yeast extract peptone dextrose, also often abbreviated as YPD, is a complete

medium Medium may refer to: Science and technology Aviation *Medium bomber, a class of war plane *Tecma Medium, a French hang glider design Communication * Media (communication), tools used to store and deliver information or data * Medium of ...

for

yeast Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom. The first yeast originated hundreds of millions of years ago, and at least 1,500 species are currently recognized. They are estimated to constit ...

growth. It contains

yeast extract Yeast extracts consist of the cell contents of yeast without the cell walls; they are used as food additives or flavorings, or as nutrients for bacterial culture media. They are often used to create savory flavors and umami taste sensations, ...

, peptone, double-distilled water, and

glucose Glucose is a simple sugar with the molecular formula . Glucose is overall the most abundant monosaccharide, a subcategory of carbohydrates. Glucose is mainly made by plants and most algae during photosynthesis from water and carbon dioxide, u ...

(dextrose). It can be used as solid medium by including agar. The yeast extract will typically contain all the

amino acid Amino acids are organic compounds that contain both amino and carboxylic acid functional groups. Although hundreds of amino acids exist in nature, by far the most important are the alpha-amino acids, which comprise proteins. Only 22 alpha a ...

s necessary for growth. By being a ''complete'' medium, YEPD cannot be used as a selection medium to test for

auxotroph Auxotrophy ( grc, αὐξάνω "to increase"; ''τροφή'' "nourishment") is the inability of an organism to synthesize a particular organic compound required for its growth (as defined by IUPAC). An auxotroph is an organism that displays this ...

s. Instead, YEPD is used as a growth medium to grow yeast cultures. YEPD typically contains 1% yeast extract, 2% peptone, and 2% glucose in distilled water. It may be made as a broth, or made into an agar gel by adding 1.5 to 2% agar.

References

Microbiological media {{microbiology-stub