Tombusvirus 5′ UTR is an important
cis-regulatory region of the
Tombus virus genome.
Tomato bushy stunt virus is the prototype member of the
Tombusviridae
''Tombusviridae'' is a family of single-stranded positive sense RNA plant viruses. There are three subfamilies, 17 genera, and 95 species in this family. The name is derived from ''Tomato bushy stunt virus'' (TBSV).
Genome
All viruses in the f ...
family. The genome of this virus is
positive sense
In molecular biology and genetics, the sense of a nucleic acid molecule, particularly of a strand of DNA or RNA, refers to the nature of the roles of the strand and its complement in specifying a sequence of amino acids. Depending on the context, ...
single stranded RNA. Replication occurs via a negative strand RNA intermediate. In addition to viral proteins p33 and the RNA-dependent RNA polymerase p92, and unknown host factors, conserved and structural regions within the
5′ untranslated region (5′ UTR) are important for regulating genome replication.
2 RNA domains in the 5′ UTR have been reported, a 5′ T-shaped domain (TSD) followed by a stem-loop (SL5)
and a downstream domain (DSD).
TSD-DSD interactions are proposed to be involved in the mediation of viral RNA replication.
An interesting feature of Tombusvirus is its ability to support the replication of defective interfering (DI) RNAs. These sub-viral replicons are small, non-coding, deletion mutants of the viral genome that maintain cis-acting RNA elements necessary for replication
Other
non-coding RNA
A non-coding RNA (ncRNA) is a functional RNA molecule that is not translated into a protein. The DNA sequence from which a functional non-coding RNA is transcribed is often called an RNA gene. Abundant and functionally important types of non-c ...
structures in Tombusvirus include the
3′ UTR region IV and an
internal replication element.
References
External links
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Cis-regulatory RNA elements
Tombusviridae
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