Shq1p is a

protein Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, respo ...

involved in the

rRNA Ribosomal ribonucleic acid (rRNA) is a type of non-coding RNA which is the primary component of ribosomes, essential to all cells. rRNA is a ribozyme which carries out protein synthesis in ribosomes. Ribosomal RNA is transcribed from ribosoma ...

processing pathway. It was discovered by Pok Yang in the Chanfreau laboratory at

UCLA The University of California, Los Angeles (UCLA) is a public land-grant research university in Los Angeles, California. UCLA's academic roots were established in 1881 as a teachers college then known as the southern branch of the California St ...

. Depletion of Shq1p has led to decreased level of various H/ACA box

snoRNA In molecular biology, Small nucleolar RNAs (snoRNAs) are a class of small RNA molecules that primarily guide chemical modifications of other RNAs, mainly ribosomal RNAs, transfer RNAs and small nuclear RNAs. There are two main classes of snoRNA, ...

s (H/ACA box snoRNAs are responsible for pseuduridylation of pre-rRNA) and certain pre-rRNA intermediates.Yang PK, Rotondo G, Porras T, Legrain P, Chanfreau G. The Shq1p.Naf1p complex is required for box H/ACA small nucleolar ribonucleoprotein particle biogenesis. J Biol Chem. 2002 Nov 22;277(47):45235-42.

Background

During the synthesis of eukaryotic ribosomes, four mature

ribosomal RNA Ribosomal ribonucleic acid (rRNA) is a type of non-coding RNA which is the primary component of ribosomes, essential to all cells. rRNA is a ribozyme which carries out protein synthesis in ribosomes. Ribosomal RNA is transcribed from ribosomal ...

s (the 5S, 5.8S, 18S, and 25S) must be synthesized. Three of these

s (5.8S, 18S, and 25S) come from a single pre-rRNA known as the 35S. Although many of the intermediates in this

processing pathway have been identified in the last thirty years, there are still a number of proteins involved in this process whose specific function is unknown.

Function

Shq1, a

thought to play a role in the stabilization and/or production of box H/ACA

, is still uncharacterized. It has been proposed that Shq1, along with Naf1p, is involved in the initial steps of the biogenesis of H/ACA box snoRNPs (box H/ACA

s form complexes with

s, thereby forming snoRNPs) because of its association with certain snoRNP proteins during the snoRNP’s maturation, while showing very little association with the mature snoRNP complex. Despite the known involvement of Shq1 in H/ACA box snoRNP's production, the exact function of this

in the overall

processing pathway is still unknown.

References

External links

Chanfreau laboratoryShq1 gene in yeast genome

{{Protein topics Molecular biology Proteins RNA

Background

Function

See also

References

External links