Putrefaction is the fifth
stage of death, following
pallor mortis __NOTOC__
Pallor mortis (Latin: ''pallor'' "paleness", ''mortis'' "of death"), the first stage of death, is an after-death paleness that occurs in those with light/white skin. An opto-electronical colour measurement device is used to measure pal ...
,
algor mortis
Algor mortis (Latin: ''algor''—coldness; ''mortis''—of death), the second stage of death, is the change in body temperature post mortem, until the ambient temperature is matched. This is generally a steady decline, although if the ambient t ...
,
rigor mortis
Rigor mortis (Latin: ''rigor'' "stiffness", and ''mortis'' "of death"), or postmortem rigidity, is the third stage of death. It is one of the recognizable signs of death, characterized by stiffening of the limbs of the corpse caused by chemic ...
, and
livor mortis
Livor mortis (Latin: ''līvor'' – "bluish color, bruise", ''mortis'' – "of death"), postmortem lividity (Latin: ''postmortem'' – "after death", ''lividity'' – "black and blue"), hypostasis (Greek: ὑπό, ''hypo'', meaning "under, bene ...
. This process references the breaking down of a body of an
animal
Animals are multicellular, eukaryotic organisms in the Kingdom (biology), biological kingdom Animalia. With few exceptions, animals Heterotroph, consume organic material, Cellular respiration#Aerobic respiration, breathe oxygen, are Motilit ...
, such as a
human
Humans (''Homo sapiens'') are the most abundant and widespread species of primate, characterized by bipedalism and exceptional cognitive skills due to a large and complex brain. This has enabled the development of advanced tools, cultu ...
,
post-mortem
An autopsy (post-mortem examination, obduction, necropsy, or autopsia cadaverum) is a surgical procedure that consists of a thorough examination of a corpse by dissection to determine the cause, mode, and manner of death or to evaluate any di ...
. In broad terms, it can be viewed as the decomposition of
protein
Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, res ...
s, and the eventual breakdown of the cohesiveness between tissues, and the liquefaction of most organs. This is caused by the decomposition of organic matter by bacterial or fungal digestion, which causes the release of gases that infiltrate the body's tissues, and leads to the deterioration of the tissues and organs.
The approximate time it takes putrefaction to occur is dependent on various factors. Internal factors that affect the rate of putrefaction include the age at which death has occurred, the overall structure and condition of the body, the cause of death, and external injuries arising before or after death. External factors include environmental temperature, moisture and air exposure, clothing, burial factors, and light exposure.
The first signs of putrefaction are signified by a greenish discoloration on the outside of the skin on the abdominal wall corresponding to where the large intestine begins, as well as under the surface of the liver.
Certain substances, such as
carbolic acid
Phenol (also called carbolic acid) is an aromaticity, aromatic organic compound with the molecular chemical formula, formula . It is a white crystalline solid that is volatility (chemistry), volatile. The molecule consists of a phenyl group () ...
,
arsenic
Arsenic is a chemical element with the symbol As and atomic number 33. Arsenic occurs in many minerals, usually in combination with sulfur and metals, but also as a pure elemental crystal. Arsenic is a metalloid. It has various allotropes, ...
,
strychnine, and
zinc chloride
Zinc chloride is the name of inorganic chemical compounds with the formula ZnCl2 and its hydrates. Zinc chlorides, of which nine crystalline forms are known, are colorless or white, and are highly soluble in water. This salt is hygroscopic and e ...
, can be used to delay the process of putrefaction in various ways based on their chemical make up.
Body farms are facilities which study the process.
Description
In
thermodynamic
Thermodynamics is a branch of physics that deals with heat, work, and temperature, and their relation to energy, entropy, and the physical properties of matter and radiation. The behavior of these quantities is governed by the four laws of the ...
terms, all organic tissues are composed of chemical energy, which, when not maintained by the constant biochemical maintenance of the living organism, begin to chemically break down due to the reaction with water into
amino acids, known as hydrolysis. The breakdown of the proteins of a decomposing body is a
spontaneous process In thermodynamics, a spontaneous process is a process which occurs without any external input to the system. A more technical definition is the time-evolution of a system in which it releases free energy and it moves to a lower, more thermodynamic ...
.
Protein hydrolysis
Hydrolyzed protein is a solution derived from the hydrolysis of a protein into its component amino acids and peptides. While many means of achieving this exist, most common is prolonged heating with hydrochloric acid, sometimes with an enzyme ...
is accelerated as the
anaerobic bacteria
An anaerobic organism or anaerobe is any organism that does not require molecular oxygen for growth. It may react negatively or even die if free oxygen is present. In contrast, an aerobic organism (aerobe) is an organism that requires an oxygenat ...
of the
digestive tract
The gastrointestinal tract (GI tract, digestive tract, alimentary canal) is the tract or passageway of the digestive system that leads from the mouth to the anus. The GI tract contains all the major organs of the digestive system, in humans and ...
consume, digest, and excrete the cellular proteins of the body.
![Rogers body](https://upload.wikimedia.org/wikipedia/commons/1/10/Rogers_body.jpg)
The bacterial digestion of the cellular proteins weakens the tissues of the body. As the proteins are continuously broken down to smaller components, the bacteria excrete gases and
organic compound
In chemistry, organic compounds are generally any chemical compounds that contain carbon-hydrogen or carbon-carbon bonds. Due to carbon's ability to catenate (form chains with other carbon atoms), millions of organic compounds are known. T ...
s, such as the
functional-group amine
In chemistry, amines (, ) are compounds and functional groups that contain a basic nitrogen atom with a lone pair. Amines are formally derivatives of ammonia (), wherein one or more hydrogen
Hydrogen is the chemical element wi ...
s
putrescine
Putrescine is an organic compound with the formula (CH2)4(NH2)2. It is a colorless solid that melts near room temperature. It is classified as a diamine. Together with cadaverine, it is largely responsible for the foul odor of putrefying flesh, ...
(from
ornithine
Ornithine is a non-proteinogenic amino acid that plays a role in the urea cycle. Ornithine is abnormally accumulated in the body in ornithine transcarbamylase deficiency. The radical is ornithyl.
Role in urea cycle
L-Ornithine is one of the produ ...
) and
cadaverine
Cadaverine is an organic compound with the formula (CH2)5(NH2)2. Classified as diamine, it is a colorless liquid with an unpleasant odor. It is present in small quantities in living organisms but is often associated with the putrefaction of an ...
(from
lysine), which carry the noxious odor of rotten flesh. Initially, the gases of putrefaction are constrained within the body cavities, but eventually diffuse through the adjacent tissues, and then into the
circulatory system
The blood circulatory system is a system of organs that includes the heart, blood vessels, and blood which is circulated throughout the entire body of a human or other vertebrate. It includes the cardiovascular system, or vascular system, tha ...
. Once in the blood vessels, the putrid gases infiltrate and diffuse to other parts of the body and the limbs.
The visual result of gaseous tissue-infiltration is notable bloating of the torso and limbs. The increased internal pressure of the continually rising volume of gas further stresses, weakens, and separates the tissues constraining the gas. In the course of putrefaction, the skin tissues of the body eventually rupture and release the bacterial gas. As the anaerobic bacteria continue consuming, digesting, and excreting the tissue proteins, the body's decomposition progresses to the stage of
skeletonization
Skeletonization is the state of a dead organism after undergoing decomposition.The Australian Museum. (2018). Decomposition-Body Changes. Retrieved from: https://australianmuseum.net.au/about/history/exhibitions/death-the-last-taboo/decomposit ...
. This continued consumption also results in the production of
ethanol
Ethanol (abbr. EtOH; also called ethyl alcohol, grain alcohol, drinking alcohol, or simply alcohol) is an organic compound. It is an alcohol with the chemical formula . Its formula can be also written as or (an ethyl group linked to a ...
by the bacteria, which can make it difficult to determine the
blood alcohol content
Blood alcohol content (BAC), also called blood alcohol concentration or blood alcohol level, is a measurement of alcohol intoxication used for legal or medical purposes; it is expressed as mass of alcohol per volume or mass of blood. For example ...
(BAC) in autopsies, particularly in bodies recovered from water.
Generally, the term ''decomposition'' encompasses the biochemical processes that occur from the physical death of the person (or animal) until the skeletonization of the body. Putrefaction is one of seven stages of
decomposition
Decomposition or rot is the process by which dead organic substances are broken down into simpler organic or inorganic matter such as carbon dioxide, water, simple sugars and mineral salts. The process is a part of the nutrient cycle and is e ...
; as such, the term ''putrescible'' identifies all organic matter (animal and human) that is
biochemically subject to putrefaction. In the matter of death by poisoning, the putrefaction of the body is chemically delayed by poisons such as
antimony
Antimony is a chemical element with the symbol Sb (from la, stibium) and atomic number 51. A lustrous gray metalloid, it is found in nature mainly as the sulfide mineral stibnite (Sb2S3). Antimony compounds have been known since ancient t ...
,
arsenic
Arsenic is a chemical element with the symbol As and atomic number 33. Arsenic occurs in many minerals, usually in combination with sulfur and metals, but also as a pure elemental crystal. Arsenic is a metalloid. It has various allotropes, ...
,
carbolic acid
Phenol (also called carbolic acid) is an aromaticity, aromatic organic compound with the molecular chemical formula, formula . It is a white crystalline solid that is volatility (chemistry), volatile. The molecule consists of a phenyl group () ...
(phenol), ''
nux vomica'' (plant),
strychnine (pesticide), and
zinc chloride
Zinc chloride is the name of inorganic chemical compounds with the formula ZnCl2 and its hydrates. Zinc chlorides, of which nine crystalline forms are known, are colorless or white, and are highly soluble in water. This salt is hygroscopic and e ...
.
Approximate timeline
![Postmortem interval changes (stages of death)](https://upload.wikimedia.org/wikipedia/commons/f/fa/Postmortem_interval_changes_%28stages_of_death%29.png)
The rough timeline of events during the putrefaction stage is as follows:
*1–2 days: Pallor mortis, algor mortis, rigor mortis, and livor mortis are the first steps in the process of decomposition before the process of putrefaction.
*2–3 days: Discoloration appears on the skin of the
abdomen
The abdomen (colloquially called the belly, tummy, midriff, tucky or stomach) is the part of the body between the thorax (chest) and pelvis, in humans and in other vertebrates. The abdomen is the front part of the abdominal segment of the to ...
. The abdomen begins to swell due to gas formation.
*3–4 days: The discoloration spreads and discolored veins become visible.
*5–6 days: The abdomen swells noticeably and the skin blisters.
*10–20 days: Black putrefaction occurs, which is when noxious odors are released from the body and the parts of the body undergo a black discoloration.
*2 weeks: The abdomen is bloated; internal gas pressure nears maximum capacity.
*3 weeks: Tissues have softened. Organs and cavities are bursting. The nails and hair fall off.
*4 weeks: Soft tissues such as the internal organs begin to
liquefy and the face becomes unrecognizable. The skin, muscles, tendons and ligaments degrade exposing the skeleton.
Order of organs' decomposition in the body:
#
Larynx and
trachea
The trachea, also known as the windpipe, is a cartilaginous tube that connects the larynx to the bronchi of the lungs, allowing the passage of air, and so is present in almost all air- breathing animals with lungs. The trachea extends from the ...
#Infant brain
#
Stomach
The stomach is a muscular, hollow organ in the gastrointestinal tract of humans and many other animals, including several invertebrates. The stomach has a dilated structure and functions as a vital organ in the digestive system. The stomach i ...
#
Intestines
The gastrointestinal tract (GI tract, digestive tract, alimentary canal) is the tract or passageway of the digestive system that leads from the mouth to the anus. The GI tract contains all the major organs of the digestive system, in humans and ...
#
Spleen
The spleen is an organ found in almost all vertebrates. Similar in structure to a large lymph node, it acts primarily as a blood filter. The word spleen comes .
#
Omentum and
mesentery
#
Liver
The liver is a major organ only found in vertebrates which performs many essential biological functions such as detoxification of the organism, and the synthesis of proteins and biochemicals necessary for digestion and growth. In humans, it ...
#Adult brain
#
Heart
The heart is a muscular organ in most animals. This organ pumps blood through the blood vessels of the circulatory system. The pumped blood carries oxygen and nutrients to the body, while carrying metabolic waste such as carbon dioxide to t ...
#
Lungs
#
Kidney
The kidneys are two reddish-brown bean-shaped organs found in vertebrates. They are located on the left and right in the retroperitoneal space, and in adult humans are about in length. They receive blood from the paired renal arteries; blo ...
s
#
Bladder
The urinary bladder, or simply bladder, is a hollow organ in humans and other vertebrates that stores urine from the kidneys before disposal by urination. In humans the bladder is a distensible organ that sits on the pelvic floor. Urine enters ...
#
Esophagus
The esophagus ( American English) or oesophagus (British English; both ), non-technically known also as the food pipe or gullet, is an organ in vertebrates through which food passes, aided by peristaltic contractions, from the pharynx to ...
#
Pancreas
The pancreas is an organ of the digestive system and endocrine system of vertebrates. In humans, it is located in the abdomen behind the stomach and functions as a gland. The pancreas is a mixed or heterocrine gland, i.e. it has both an en ...
#
Diaphragm
#
Blood vessel
The blood vessels are the components of the circulatory system that transport blood throughout the human body. These vessels transport blood cells, nutrients, and oxygen to the tissues of the body. They also take waste and carbon dioxide away ...
s
#
Uterus
The uterus (from Latin ''uterus'', plural ''uteri'') or womb () is the organ in the reproductive system of most female mammals, including humans that accommodates the embryonic and fetal development of one or more embryos until birth. The ...
The rate of putrefaction is greatest in air, followed by water, soil, and earth. The exact rate of putrefaction is dependent upon many factors such as weather, exposure and location. Thus, refrigeration at a morgue or funeral home can retard the process, allowing for burial in three days or so following death without
embalming
Embalming is the art and science of preserving human remains by treating them (in its modern form with chemicals) to forestall decomposition. This is usually done to make the deceased suitable for public or private viewing as part of the funeral ...
. The rate increases dramatically in tropical climates. The first external sign of putrefaction in a body lying in air is usually a greenish discoloration of the skin over the region of the
cecum
The cecum or caecum is a pouch within the peritoneum that is considered to be the beginning of the large intestine. It is typically located on the right side of the body (the same side of the body as the appendix, to which it is joined). The wo ...
, which appears in 12–24 hours. The first internal sign is usually a greenish discoloration on the undersurface of liver.
Factors affecting putrefaction
Various factors affect the rate of putrefaction.
Exogenous (external)
Environmental temperature: Decomposition is accelerated by high atmospheric or environmental temperature, with putrefaction speed optimized between and , further sped along by high levels of humidity. This optimal temperature assists in the chemical breakdown of the tissue and promotes microorganism growth. Decomposition nearly stops below or above .
Moisture and air exposure: Putrefaction is ordinarily slowed by the body being submerged in water, due to diminished exposure to air. Air exposure and moisture can both contribute to the introduction and growth of microorganisms, speeding degradation. In a hot and dry environment, the body can undergo a process called mummification where the body is completely dehydrated and bacterial decay is inhibited.
Clothing: Loose-fitting clothing can speed up the rate of putrefaction, as it helps to retain body heat. Tight-fitting clothing can delay the process by cutting off blood supply to tissues and eliminating nutrients for bacteria to feed on.
Manner of burial: Speedy burial can slow putrefaction. Bodies within deep graves tend to decompose more slowly due to the diminished influences of changes in temperature. The composition of graves can also be a significant contributing factor, with dense, clay-like soil tending to speed putrefaction while dry and sandy soil slows it.
Light exposure: Light can also contribute indirectly, as flies and insects prefer to lay eggs in areas of the body not exposed to light, such as the crevices formed by the eyelids and nostrils.
Endogenous (internal)
Age at time of death: Stillborn fetuses and infants putrefy slowly due to their sterility. Otherwise, however, younger people generally putrefy more quickly than older people.
Condition of the body: A body with a greater fat percentage and less lean body mass will have a faster rate of putrefaction, as fat retains more heat and it carries a larger amount of fluid in the tissues.
Cause of death: The cause of death has a direct relationship to putrefaction speed, with bodies that died from acute violence or accident generally putrefying slower than those that died from infectious diseases. Certain poisons, such as
potassium cyanide or
strychnine, may also delay putrefaction, while chronic alcoholism will speed it.
External injuries: Antemortem or postmortem injuries can speed putrefaction as injured areas can be more susceptible to invasion by bacteria.
Delayed putrefaction
Certain poisonous substances to the body can delay the process of putrefaction. They include:
*
Carbolic acid
Phenol (also called carbolic acid) is an aromaticity, aromatic organic compound with the molecular chemical formula, formula . It is a white crystalline solid that is volatility (chemistry), volatile. The molecule consists of a phenyl group () ...
(Phenol)
*
Arsenic
Arsenic is a chemical element with the symbol As and atomic number 33. Arsenic occurs in many minerals, usually in combination with sulfur and metals, but also as a pure elemental crystal. Arsenic is a metalloid. It has various allotropes, ...
and
antimony
Antimony is a chemical element with the symbol Sb (from la, stibium) and atomic number 51. A lustrous gray metalloid, it is found in nature mainly as the sulfide mineral stibnite (Sb2S3). Antimony compounds have been known since ancient t ...
*
Strychnine
*
Nux vomica (plant)
*
Zinc chloride
Zinc chloride is the name of inorganic chemical compounds with the formula ZnCl2 and its hydrates. Zinc chlorides, of which nine crystalline forms are known, are colorless or white, and are highly soluble in water. This salt is hygroscopic and e ...
, ZnCl
2
Embalming
Embalming
Embalming is the art and science of preserving human remains by treating them (in its modern form with chemicals) to forestall decomposition. This is usually done to make the deceased suitable for public or private viewing as part of the funeral ...
is the process of preserving human remains by delaying decomposition. This is acquired through the use of embalming fluid, which is a mixture of formaldehyde, methanol, and various other solvents. The most common reasons to preserve the body are for viewing purposes at a funeral, for above-ground interment or distant transportation of the deceased, and for medical or religious practices.
Research
Body farms subject donated cadavers to various environmental conditions to study the process of human decomposition. These include The University of Tennessee's Forensic Anthropologic Facility, Western Carolina Universities Osteology Research Station (FOREST), Texas State University's Forensic Anthropology Research Facility (FARF), Sam Houston State University's Southeast Texas Applied Forensic Science Facility (STAFS), Southern Illinois University's Complex for Forensic Anthropology Research, and Colorado Mesa University's Forensic Investigation Research Station. The Australian Facility for Taphonomic Experimental Research, near
Sydney, is the first body farm located outside of the United States In The United Kingdom there are several facilities which, instead of using human remains or cadavers, use dead pigs to study the decomposition process. Pigs are less likely to have infectious diseases than human cadavers, and are more readily available without any concern for ethical issues, but a human body farm is still highly sought after for further research.
Each body farm is unique in its environmental make-up, giving researchers a broader knowledge, and allowing research into how different environmental factors can affect the rate of decomposition significantly such as humidity, sun exposure, rain or snow, altitude level and more.
Other uses
![Musaeum Hermeticum 1678 VIII](https://upload.wikimedia.org/wikipedia/commons/2/28/Musaeum_Hermeticum_1678_VIII._Clavis_0066.JPG)
In
alchemy
Alchemy (from Arabic: ''al-kīmiyā''; from Ancient Greek: χυμεία, ''khumeía'') is an ancient branch of natural philosophy, a philosophical and protoscientific tradition that was historically practiced in China, India, the Muslim world, ...
, putrefaction is the same as
fermentation, whereby a substance is allowed to rot or decompose undisturbed. In some cases, the commencement of the process is facilitated with a small sample of the desired material to act as a "seed", a technique akin to the use of a
seed crystal in
crystallization.
See also
*
Cryopreservation
Cryo-preservation or cryo-conservation is a process where organisms, organelles, cells, tissues, extracellular matrix, organs, or any other biological constructs susceptible to damage caused by unregulated chemical kinetics are preserved by co ...
*
Corpse decomposition Decomposition is the process in which the organs and complex molecules of animal and human bodies break down into simple organic matter over time. In vertebrates, five stages of decomposition are typically recognized: fresh, bloat, active decay, ...
*
Decomposition
Decomposition or rot is the process by which dead organic substances are broken down into simpler organic or inorganic matter such as carbon dioxide, water, simple sugars and mineral salts. The process is a part of the nutrient cycle and is e ...
*
Forensic entomological decomposition
*
Maceration (bone)
Maceration is a bone preparation technique whereby a clean skeleton is obtained from a vertebrate carcass by leaving it to decompose inside a closed container at near-constant temperature. This may be done as part of a forensic investigation, as ...
*
Promession
*
Putrefying bacteria
*
Rancidification
Rancidification is the process of complete or incomplete autoxidation or hydrolysis of fats and oils when exposed to air, light, moisture, or bacterial action, producing short-chain aldehydes, ketones and free fatty acids.
When these processes o ...
*
Cotard delusion
Cotard's syndrome, also known as Cotard's delusion or walking corpse syndrome, is a rare mental disorder in which the affected person holds the delusional belief that they are dead, do not exist, are putrefying, or have lost their blood or inter ...
References
{{Reflist
External links
Putrefaction Dr. Dinesh Rao's Forensic Pathology
Alchemical processes
Food science
Medical aspects of death
Necrosis
Forensic pathology