The pGreen plasmids are vectors for plant transformation. They were first described in 2000 as components of a novel T-DNA binary system. The supporting web page provides supplementary information and ongoing support to researchers to request their plasmid resources. As these plasmids have been taken up by the research community, the plasmids have been developed, expanding the resources available to the community. Researchers are encouraged to contribute to this research community by submitting their vector sequence to

genbank The GenBank sequence database is an open access, annotated collection of all publicly available nucleotide sequences and their protein translations. It is produced and maintained by the National Center for Biotechnology Information (NCBI; a part ...

and providing a description of the plasmid on the site.

pGreenI and pGreenII

pGreen is the original pGreen plasmid. pGreenII features

plasmid backbone modification A plasmid is a small, extrachromosomal DNA molecule within a cell that is physically separated from chromosomal DNA and can replicate independently. They are most commonly found as small circular, double-stranded DNA molecules in bacteria; how ...

to improve plasmid stability.

T-DNA regions

No transformation selection

pGreenII 0000: minimal T-DNA with Left and Right border, ''lacZ'' gene for blue/white selection during cloning multiple cloning site derived from pBluescript. pGreenII 62-SK: derived from pGreenII 0000, the ''Lac''Z blue/white cloning selection has been replaced with a 35S- MCS- CaMV cassette that allows the insertion of a gene of interest into a 35S over-expression cassette. The multiple cloning site (MCS) is derived from pBluescript.

Kanamycin selection

pGreenII 0029: derived from pGreenII 0000, a nos-kan cassette has been inserted into the ''Hpa''I site of the Left Border, providing resistance to

kanamycin Kanamycin A, often referred to simply as kanamycin, is an antibiotic used to treat severe bacterial infections and tuberculosis. It is not a first line treatment. It is used by mouth, injection into a vein, or injection into a muscle. Kanamyci ...

during plant transformation selection. pGreenII 0029 62-SK: derived from pGreenII 0029, the ''Lac''Z blue/white cloning selection has been replaced with a 35S-MCS-CaMV cassette that allows the insertion of a gene of interest into a 35S over-expression cassette. The MCS is derived from pBluescript.

Hygromycin selection

pGreenII 0179: derived from pGreenII 0000, a 35S-hyg cassette has been inserted into the ''Hpa''I site of the Left Border, providing resistance to

hygromycin Hygromycin may refer to either of two chemically dissimilar antibiotics produced by the bacterium '' Streptomyces hygroscopicus'': * Hygromycin A * Hygromycin B, an aminoglycoside that kills bacteria, fungi and higher eukaryotic Eukaryotes ...

during plant transformation selection.

Bialaphos selection

pGreenII 0229: derived from pGreenII 0000, a nos-bar cassette has been inserted into the ''Hpa''I site of the Left Border, providing resistance to

bialaphos Bialaphos is a natural herbicide produced by the bacteria ''Streptomyces hygroscopicus'' and ''Streptomyces viridochromogenes''. Bialaphos is a protoxin and nontoxic as is. When it is metabolized by the plant, the glutamic acid analog glufosinate ...

or phosphinothricin during plant transformation selection. pGreenII 0229 62-SK: derived from pGreenII 0229, the ''Lac''Z blue/white cloning selection has been replaced with a 35S-MCS-CaMV cassette that allows the insertion of a gene of interest into a 35S over-expression cassette. The MCS is derived from pBluescript.

pSoup

This is the helper plasmid that provides the replicase function for the pSa replication origin of pGreen. pSoup is tetracyclin resistant and a complementary incompatibility group such that it can co-exist with pGreen in the ''

Agrobacterium ''Agrobacterium'' is a genus of Gram-negative bacteria established by H. J. Conn that uses horizontal gene transfer to cause tumors in plants. '' Agrobacterium tumefaciens'' is the most commonly studied species in this genus. ''Agrobacterium'' i ...

'' cell. pSoup: the original help plasmid for pGreen. pGreen will not replicate in ''Agrobacterium'' if it is not present.

References

External links

The pGreen research page
{{DEFAULTSORT:Pgreen Biotechnology