''o''-Dianisidine is an organic compound with the formula CH₃O)(H₂N)C₆H₃sub>2. A colorless or white solid, it is a bifunctional compound derived via the

benzidine rearrangement Benzidine (trivial name), also called 1,1'-biphenyl-4,4'-diamine (systematic name), is an organic compound with the formula (C6H4NH2)2. It is an aromatic amine. It is a component of a test for cyanide. Related derivatives are used in the produ ...

from ''o''-anisidine. ''o''-Dianisidine is a precursor to some azo dyes by formation of the bis(

diazonium Diazonium compounds or diazonium salts are a group of organic compounds sharing a common functional group where R can be any organic group, such as an alkyl or an aryl, and X is an inorganic or organic anion, such as a halide. General properti ...

) derivative, which is coupled to diverse aromatic compounds. Some commercial dyes derived from ''o''-dianisidine include C. I.

Direct Blue 1 Direct Blue 1 is an organic compound that is one of many azo dyes. This salt is used as a substantive dye A substantive dye or direct dye is a dye that adheres to its substrate, typically a textile, by non-ionic forces. Overview The amoun ...

, 15, 22, 84, and 98.. ''o''-Dianisidine is also used in assaying activity of

peroxidase Peroxidases or peroxide reductases ( EC numberbr>1.11.1.x are a large group of enzymes which play a role in various biological processes. They are named after the fact that they commonly break up peroxides. Functionality Peroxidases typically ca ...

in lab. The general reaction of a peroxidase is as follows. :ROOR' + \overset + 2H+ -> ce + R'OH Where the ROOR' can be

hydrogen peroxide Hydrogen peroxide is a chemical compound with the formula . In its pure form, it is a very pale blue liquid that is slightly more viscous than water. It is used as an oxidizer, bleaching agent, and antiseptic, usually as a dilute solution (3%� ...

, and the electron donor be ''o''-dianisidine.

Safety

The manufacture and degradation of ''o''-dianisidine, like other benzidene derivatives, has attracted regulatory attention. It is also used as a reagent in biochemistry in testing for

peroxide In chemistry, peroxides are a group of compounds with the structure , where R = any element. The group in a peroxide is called the peroxide group or peroxo group. The nomenclature is somewhat variable. The most common peroxide is hydrogen ...

References

{{DEFAULTSORT:Dianisidine, o- Anilines IARC Group 1 carcinogens Biphenyls Diamines