NSP5 ( nonstructural protein 5) encoded by genome segment 11 of group A

rotavirus ''Rotavirus'' is a genus of double-stranded RNA viruses in the family ''Reoviridae''. Rotaviruses are the most common cause of diarrhoeal disease among infants and young children. Nearly every child in the world is infected with a rotavirus ...

es. In virus-infected cells NSP5 accumulates in the

viroplasm A viroplasm, sometimes called "virus factory" or "virus inclusion", is an inclusion body in a cell where viral replication and assembly occurs. They may be thought of as viral factories in the cell. There are many viroplasms in one infected cell, w ...

s. NSP5 has been shown to be autophosphorylated. Interaction of NSP5 with NSP2 was also demonstrated. In

-infected cells, the non-structural proteins NSP5 and NSP2 localize in complexes called

s, where replication and assembly occur and they can drive the formation of viroplasm-like structures in the absence of other rotaviral proteins and rotavirus replication. There is no atomic-resolution structure of NSP5 determined as of June 2019. However, the low resolution three-dimensional structure of the NSP2-NSP5 assembly has been observed by

cryo-EM Cryogenic electron microscopy (cryo-EM) is a cryomicroscopy technique applied on samples cooled to cryogenic temperatures. For biological specimens, the structure is preserved by embedding in an environment of vitreous ice. An aqueous sample so ...

. NSP5 occupies the same site as RNA when binding to NSP2. The EM data from this 2006 study has not been published.

References

Rotaviruses Viral nonstructural proteins {{enzyme-stub