Novichok
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Novichok (russian: Новичо́к, lit=newcomer, novice, newbie) is a group of
nerve agents Nerve agents, sometimes also called nerve gases, are a class of organic chemicals that disrupt the mechanisms by which nerves transfer messages to organs. The disruption is caused by the blocking of acetylcholinesterase (AChE), an enzyme that ...
, some of which are binary chemical weapons. The agents were developed at the
GosNIIOKhT The State Research Institute of Organic Chemistry and Technology () (GosNIIOKhT) is a Russian research institute engaged in the development of chemical technologies for use in the national economy and the production of relevant goods and pro ...
state chemical research institute by the
Soviet Union The Soviet Union,. officially the Union of Soviet Socialist Republics. (USSR),. was a transcontinental country that spanned much of Eurasia from 1922 to 1991. A flagship communist state, it was nominally a federal union of fifteen national ...
and Russia between 1971 and 1993. Some Novichok agents are
solids Solid is one of the four fundamental states of matter (the others being liquid, gas, and plasma). The molecules in a solid are closely packed together and contain the least amount of kinetic energy. A solid is characterized by structural ri ...
at
standard temperature and pressure Standard temperature and pressure (STP) are standard sets of conditions for experimental measurements to be established to allow comparisons to be made between different sets of data. The most used standards are those of the International Union o ...
, while others are
liquids A liquid is a nearly incompressible fluid that conforms to the shape of its container but retains a (nearly) constant volume independent of pressure. As such, it is one of the four fundamental states of matter (the others being solid, gas, an ...
. Dispersal of solid form agents is thought possible if in ultrafine powder state. Russian scientists who developed the nerve agents claim they are the deadliest ever made, with some variants possibly five to eight times more potent than VX, and others up to ten times more potent than
soman Soman (or GD, EA 1210, Zoman, PFMP, A-255, systematic name: ''O''-pinacolyl methylphosphonofluoridate) is an extremely toxic chemical substance. It is a nerve agent, interfering with normal functioning of the mammalian nervous system by inhibiti ...
. As well as Russia, Novichok agents have been known to be produced in
Iran Iran, officially the Islamic Republic of Iran, and also called Persia, is a country located in Western Asia. It is bordered by Iraq and Turkey to the west, by Azerbaijan and Armenia to the northwest, by the Caspian Sea and Turkmeni ...
. In the 21st century, Novichok agents came to public attention after they were used to poison opponents of the Russian government, including the Skripals and two others in Amesbury, UK (2018), and
Alexei Navalny Alexei Anatolievich Navalny ( rus, links=no, Алексей Анатольевич Навальный, , ɐlʲɪkˈsʲej ɐnɐˈtolʲjɪvʲɪtɕ nɐˈvalʲnɨj; born 4 June 1976) is a Opposition to Vladimir Putin in Russia, Russian opposition ...
(2020), but civil poisonings with this substance have been known since at least 1995. In November 2019, the
Organisation for the Prohibition of Chemical Weapons The Organisation for the Prohibition of Chemical Weapons (OPCW) is an intergovernmental organisation and the implementing body for the Chemical Weapons Convention (CWC), which entered into force on 29 April 1997. The OPCW, with its 193 member ...
(OPCW), which is the executive body for the
Chemical Weapons Convention The Chemical Weapons Convention (CWC), officially the Convention on the Prohibition of the Development, Production, Stockpiling and Use of Chemical Weapons and on their Destruction, is an arms control treaty administered by the Organisation for ...
(CWC), added the Novichok agents to "list of controlled substances" of the CWC "in one of the first major changes to the treaty since it was agreed in the 1990s" in response to the 2018 poisonings in the UK.


Design objectives

Novichok agents were designed to achieve four objectives: * to be undetectable using standard 1970s and 1980s
NATO The North Atlantic Treaty Organization (NATO, ; french: Organisation du traité de l'Atlantique nord, ), also called the North Atlantic Alliance, is an intergovernmental military alliance between 30 member states – 28 European and two No ...
chemical detection equipment; * to defeat NATO chemical protective gear; * to be safer to handle; and * to circumvent the
Chemical Weapons Convention The Chemical Weapons Convention (CWC), officially the Convention on the Prohibition of the Development, Production, Stockpiling and Use of Chemical Weapons and on their Destruction, is an arms control treaty administered by the Organisation for ...
list of controlled precursors, classes of chemical and physical form. Some of these agents are binary weapons, in which precursors for the nerve agents are mixed in a munition to produce the agent just prior to its use. The precursors are generally significantly less hazardous than the agents themselves, so this technique makes handling and transporting the munitions a great deal simpler. Additionally, precursors to the agents are usually much easier to stabilise than the agents themselves, so this technique also makes it possible to increase the
shelf life Shelf life is the length of time that a commodity may be stored without becoming unfit for use, consumption, or sale. In other words, it might refer to whether a commodity should no longer be on a pantry shelf (unfit for use), or no longer on a ...
of the agents. This has the disadvantage that careless preparation may produce a non-optimal agent. During the 1980s and 1990s, binary versions of several Soviet agents were developed and are designated as "Novichok" agents.


History and disclosure

Novichok agents were designed as part of a Soviet program codenamed Foliant. Five Novichok variants are believed to have been adapted for military use. The most versatile is
A-232 A-232 is an organophosphate nerve agent. It was developed in the Soviet Union under the FOLIANT program and is one of the group of compounds referred to as Novichok agents that were revealed by Vil Mirzayanov. A-232 is reportedly slightly less pot ...
(Novichok-5). Novichok agents have never been used on the battlefield. The UK government determined that a novichok agent was used in the
poisoning of Sergei and Yulia Skripal On 4 March 2018, Sergei Skripal, a former Russian military officer and double agent for the British intelligence agencies, and his daughter, Yulia Skripal, were poisoned in the city of Salisbury, England. According to UK sources and the Organi ...
in Salisbury, Wiltshire, England in March 2018. It was unanimously confirmed by four laboratories around the world, according to the OPCW. Novichok was also involved in the poisoning of a British couple in Amesbury, Wiltshire, four months later, believed to have been caused by nerve agent discarded after the Salisbury attack. The attacks led to the death of one person, left three others in a critical condition from which they recovered, and briefly hospitalised a police officer. The Russian government denies producing or researching agents "under the title Novichok". In September 2020, the
German German(s) may refer to: * Germany (of or related to) ** Germania (historical use) * Germans, citizens of Germany, people of German ancestry, or native speakers of the German language ** For citizens of Germany, see also German nationality law **Ge ...
government said that opposition figure and anti-corruption activist
Alexei Navalny Alexei Anatolievich Navalny ( rus, links=no, Алексей Анатольевич Навальный, , ɐlʲɪkˈsʲej ɐnɐˈtolʲjɪvʲɪtɕ nɐˈvalʲnɨj; born 4 June 1976) is a Opposition to Vladimir Putin in Russia, Russian opposition ...
, who was evacuated from
Omsk Omsk (; rus, Омск, p=omsk) is the administrative center and largest city of Omsk Oblast, Russia. It is situated in southwestern Siberia, and has a population of over 1.1 million. Omsk is the third largest city in Siberia after Novosibirsk ...
to
Berlin Berlin ( , ) is the capital and largest city of Germany by both area and population. Its 3.7 million inhabitants make it the European Union's most populous city, according to population within city limits. One of Germany's sixteen constitue ...
for treatment in late August after becoming ill during his flight, was poisoned by a Novichok agent. Novichok has been known to most western intelligence services since the 1990s, and in 2016 Iranian chemists working at a university in Tehran synthesised five of the seven Novichok agents for analysis and produced detailed mass spectral data which was added to the OPCW's Central Analytical Database. Previously, there had been no detailed descriptions of their spectral properties in open scientific literature. A small amount of agent
A-230 A-230 is an organophosphate nerve agent. It was developed in the Soviet Union under the FOLIANT program and is one of the group of compounds referred to as Novichok agents that were revealed by Vil Mirzayanov. A-230 is possibly the most potent ner ...
was also claimed to have been synthesised in the Czech Republic in 2017 for the purpose of obtaining analytical data to help defend against these novel toxic compounds. The Soviet Union and Russia reportedly developed extremely potent fourth-generation chemical weapons from the 1970s until the early 1990s, according to a publication by two chemists, Lev Fyodorov and
Vil Mirzayanov Vil Sultanovich Mirzayanov (russian: Вил Султанович Мирзаянов, tt-Cyrl, Вил Солтан улы Мирзаҗанов; born 9 March 1935 in Starokangyshevo, Dyurtyulinsky District, Bashkortostan) is a Russian people, Russi ...
in ''
Moskovskiye Novosti ''Moskovskiye Novosti'' (russian: Московские новости, ''Moscow News'') was a Russian-language daily newspaper in Russia relaunched in 2011. The paper - by then a 'youth-oriented' free sheet handed out at more than 850 places aro ...
'' weekly in 1992. The publication appeared just on the eve of Russia's signing of the
Chemical Weapons Convention The Chemical Weapons Convention (CWC), officially the Convention on the Prohibition of the Development, Production, Stockpiling and Use of Chemical Weapons and on their Destruction, is an arms control treaty administered by the Organisation for ...
. According to Mirzayanov, the Russian Military Chemical Complex (MCC) was using defence conversion money received from the West for development of a chemical warfare facility. Mirzayanov made his disclosure out of environmental concerns. He was the head of a counter-intelligence department and performed measurements outside the chemical weapons facilities to make sure that foreign spies could not detect any traces of production. To his horror, the levels of deadly substances were eighty times greater than the maximum safe concentration. The
Prosecutor-General of Russia The Prosecutor General of Russia (also Attorney General of Russia, russian: Генеральный прокурор Российской Федерации, Generalʹnyy prokuror Rossiyskoy Federatsii) heads the system of official prosecution ...
effectively admitted the existence of Novichok agents when he brought a
treason Treason is the crime of attacking a state authority to which one owes allegiance. This typically includes acts such as participating in a war against one's native country, attempting to overthrow its government, spying on its military, its diplo ...
case against Mirzayanov. According to expert witness testimonies that three scientists prepared for the
KGB The KGB (russian: links=no, lit=Committee for State Security, Комитет государственной безопасности (КГБ), a=ru-KGB.ogg, p=kəmʲɪˈtʲet ɡəsʊˈdarstvʲɪn(ː)əj bʲɪzɐˈpasnəsʲtʲɪ, Komitet gosud ...
, Novichok and other related chemical agents had indeed been produced and therefore Mirzayanov's disclosure represented
high treason Treason is the crime of attacking a state authority to which one owes allegiance. This typically includes acts such as participating in a war against one's native country, attempting to overthrow its government, spying on its military, its diplo ...
. Mirzayanov was arrested on 22 October 1992 and sent to Lefortovo prison for divulging state secrets. He was released later because "not one of the formulas or names of poisonous substances in the ''Moscow News'' article was new to the Soviet press, nor were locations ... of testing sites revealed." According to
Yevgenia Albats Yevgenia Markovna Albats (russian: Евге́ния Ма́рковна Альба́ц, born 5 September 1958state secret revealed by Fyodorov and Mirzayanov was that generals had lied—and were still lying—to both the international community and their fellow citizens." Mirzayanov now lives in the U.S. Further disclosures followed when Vladimir Uglev, one of Russia's leading binary weapons scientists, revealed the existence of A-232/Novichok-5 in an interview with the magazine '' Novoye Vremya'' in early 1994. In his 1998 interview with David E. Hoffman for ''
The Washington Post ''The Washington Post'' (also known as the ''Post'' and, informally, ''WaPo'') is an American daily newspaper published in Washington, D.C. It is the most widely circulated newspaper within the Washington metropolitan area and has a large nati ...
'' the chemist claimed that he helped invent the A-232 agent, that it was more frostproof, and confirmed that a binary version has been developed from it. Uglev revealed more details in 2018, following the poisoning of the Skripals, stating that "several hundred" compounds were synthesised during the Foliant research but only four agents were weaponised (presumably the Novichok-5, −7, −8 and −9 mentioned by other sources): the first three were liquids and only the last, which was not developed until 1980, could be made into a powder. Unlike the interview twenty years earlier, he denied any binary agents were developed successfully, at least up until his involvement in the research ceased in 1994. In the 1990s, the German
Federal Intelligence Service The Federal Intelligence Service (German: ; , BND) is the foreign intelligence agency of Germany, directly subordinate to the Chancellor's Office. The BND headquarters is located in central Berlin and is the world's largest intelligence head ...
(BND) obtained a sample of one Novichok agent from a Russian scientist, and the sample was analysed in Sweden, according to a 2018 ''
Reuters Reuters ( ) is a news agency owned by Thomson Reuters Corporation. It employs around 2,500 journalists and 600 photojournalists in about 200 locations worldwide. Reuters is one of the largest news agencies in the world. The agency was estab ...
'' report. The chemical formula was given to Western
NATO The North Atlantic Treaty Organization (NATO, ; french: Organisation du traité de l'Atlantique nord, ), also called the North Atlantic Alliance, is an intergovernmental military alliance between 30 member states – 28 European and two No ...
countries, who synthesized it, then used small amounts to test protective equipment, detection of it, and antidotes to it. Novichok was referred to in a patent filed in 2008 for an organophosphorus poisoning treatment. The
University of Maryland, Baltimore The University of Maryland, Baltimore (UMB) is a public university in Baltimore, Maryland. Founded in 1807, it comprises some of the oldest professional schools of dentistry, law, medicine, pharmacy, social work and nursing in the United States ...
research was part-funded by the
U.S. Army The United States Army (USA) is the land service branch of the United States Armed Forces. It is one of the eight U.S. uniformed services, and is designated as the Army of the United States in the U.S. Constitution.Article II, section 2, cl ...
. Professor Leonid Rink, who said he had participated in the creation of Novichok agents, confirmed that the structures leaked by Mirzayanov were the correct ones. Rink was himself convicted in Russia for illegally selling a Novichok agent used to assassinate in 1995 a banker Ivan Kivelidi and his secretary. David Wise, in his book ''Cassidy's Run'', implies that the Soviet program may have been the unintended result of misleading information, involving a discontinued American program to develop a nerve agent code named " GJ", that was fed by a double agent to the Soviets as part of Operation Shocker.


Development and test sites

Stephanie Fitzpatrick, an American geopolitical consultant, has claimed that the Chemical Research Institute in
Nukus Nukus ( kaa, Nókis / ; uz, Nukus / ; kk, Нүкіс / ) is the sixth-largest city in Uzbekistan and the capital of the autonomous Republic of Karakalpakstan. The population of Nukus as of January 1, 2022 was 329,100. The Amu Darya river passes ...
,
Soviet Uzbekistan Uzbekistan (, ) is the common English name for the Uzbek Soviet Socialist Republic (Uzbek SSR; uz, Ўзбекистон Совет Социалистик Республикаси, Oʻzbekiston Sovet Sotsialistik Respublikasi, in Russian: Уз ...
produced Novichok agents and ''
The New York Times ''The New York Times'' (''the Times'', ''NYT'', or the Gray Lady) is a daily newspaper based in New York City with a worldwide readership reported in 2020 to comprise a declining 840,000 paid print subscribers, and a growing 6 million paid ...
'' has reported that U.S. officials said the site was the major research and testing site for Novichok agents. Small, experimental batches of the weapons may have been tested on the nearby
Ustyurt Plateau The Ustyurt or Ust-Yurt (from kk, Үстірт; uz, Ustyurt; tk, Üstyurt; — flat hill, plateau) is a transboundary clay desert shared by Turkmenistan, Uzbekistan and Kazakhstan. The plateau's semi-nomadic population raises sheep, goats, an ...
. Fitzpatrick also writes that the agents may have been tested in a research centre in Krasnoarmeysk near Moscow. Precursor chemicals were made at the Pavlodar Chemical Plant in
Soviet Kazakhstan ; kk, Қазақ Советтік Социалистік Республикасы) *1991: Republic of Kazakhstan (russian: Республика Казахстан; kk, Қазақстан Республикасы) , linking_name = the ...
, which was also thought to be the intended Novichok weapons production site, until its still-under-construction chemical warfare agent production building was demolished in 1987 in view of the forthcoming 1990 Chemical Weapons Accord and the
Chemical Weapons Convention The Chemical Weapons Convention (CWC), officially the Convention on the Prohibition of the Development, Production, Stockpiling and Use of Chemical Weapons and on their Destruction, is an arms control treaty administered by the Organisation for ...
. Since its independence in 1991,
Uzbekistan Uzbekistan (, ; uz, Ozbekiston, italic=yes / , ; russian: Узбекистан), officially the Republic of Uzbekistan ( uz, Ozbekiston Respublikasi, italic=yes / ; russian: Республика Узбекистан), is a doubly landlocked cou ...
has been working with the government of the United States to dismantle and decontaminate the sites where the Novichok agents and other chemical weapons were tested and developed. Between 1999 and 2002 the
United States Department of Defense The United States Department of Defense (DoD, USDOD or DOD) is an executive branch department of the federal government charged with coordinating and supervising all agencies and functions of the government directly related to national secu ...
dismantled the major research and testing site for Novichok at the Chemical Research Institute in Nukus, under a $6 million Cooperative Threat Reduction programme. Hamish de Bretton-Gordon, a British chemical weapons expert and former commanding officer of the UK's
Joint Chemical, Biological, Radiation and Nuclear Regiment The Joint Chemical, Biological, Radiological and Nuclear Regiment was a specialist expeditionary unit of the British armed forces. Personnel of the Joint CBRN Regiment were trained in the detection, identification and monitoring of nuclear, biolo ...
and its NATO equivalent, "dismissed" suggestions that Novichok agents could be found in other places in the former Soviet Union such as Uzbekistan and has asserted that Novichok agents were produced only at
Shikhany Shikhany (russian: Шиханы) is a town in Saratov Oblast, Russia, located north of Saratov on the right bank of the Volga River Population: . It has been a closed town since 1997, but lost this status on 1 January 2019. The town is located 2 ...
in
Saratov Oblast Saratov Oblast (russian: Сара́товская о́бласть, ''Saratovskaya oblast'') is a federal subjects of Russia, federal subject of Russia (an oblast), located in the Volga Federal District. Its administrative center is the types of ...
, Russia. Mirzayanov also says that it was at Shikhany, in 1973, that scientist Pyotr Petrovich Kirpichev first produced Novichok agents; Vladimir Uglev joined him on the project in 1975. According to Mirzayanov, while production took place in Shikhany, the weapon was ''tested'' at Nukus between 1986 and 1989. Following the poisoning of the Skripals, former head of the GosNIIOKhT security department Nikolay Volodin confirmed in an interview to ''
Novaya Gazeta ''Novaya Gazeta'' ( rus, Новая газета, t=New Gazette, p=ˈnovəjə ɡɐˈzʲetə) is an independent Russian newspaper known for its critical and investigative coverage of Russian political and social affairs. It is published in Mo ...
'' that there have been tests at Nukus, and said that dogs were used. In May 2018, the ''Irish Independent'' reported that "Germany's foreign intelligence service secured a sample of the Soviet-developed nerve agent Novichok in the 1990s and passed on its knowledge to partners including Britain and the US, according to German media reports." The sample was analysed in Sweden. Small amounts of the Novichok nerve agent were subsequently produced in some
NATO The North Atlantic Treaty Organization (NATO, ; french: Organisation du traité de l'Atlantique nord, ), also called the North Atlantic Alliance, is an intergovernmental military alliance between 30 member states – 28 European and two No ...
countries for test purposes.


Description of Novichok agents

Mirzayanov provided the first description of these agents. Dispersed in an ultra-fine
powder A powder is a dry, bulk solid composed of many very fine particles that may flow freely when shaken or tilted. Powders are a special sub-class of granular materials, although the terms ''powder'' and ''granular'' are sometimes used to distin ...
instead of a gas or a vapour, they have unique qualities. A binary agent was then created that would mimic the same properties but would either be manufactured using materials which are not controlled substances under the CWC, or be undetectable by treaty regime inspections. The most potent compounds from this family, Novichok-5 and Novichok-7, are supposedly around five to eight times more potent than VX. The "Novichok" designation refers to the binary form of the agent, with the final compound being referred to by its code number (e.g. A-232). The first Novichok series compound was in fact the binary form of a known V-series nerve agent, VR, while the later Novichok agents are the binary forms of compounds such as A-232 and A-234. According to a classified (secret) report by the US Army
National Ground Intelligence Center The National Ground Intelligence Center (NGIC) is part of the United States Army Intelligence and Security Command. The NGIC provides scientific and technical intelligence (S&T) and general military intelligence (GMI) on foreign ground forces i ...
in '' Military Intelligence Digest'' dated 24 January 1997, agent designated A-232 and its ethyl analogue A-234 developed under the Foliant programme "are as toxic as VX, as resistant to treatment as soman, and more difficult to detect and easier to manufacture than VX". The binary versions of the agents reportedly use
acetonitrile Acetonitrile, often abbreviated MeCN (methyl cyanide), is the chemical compound with the formula and structure . This colourless liquid is the simplest organic nitrile (hydrogen cyanide is a simpler nitrile, but the cyanide anion is not clas ...
and an organic phosphate "that can be disguised as a pesticide precursor." The agent A-234 is also supposedly around five to eight times more potent than VX. The median lethal dose for inhaled A-234 has been estimated as 7 mg/m3 for two minute exposure (minute volume of 15 L, slight activity). The median lethal dose for inhaled A-230, likely the most toxic liquid Novichok, has been estimated as between 1.9 and 3 mg/m3 for two minute exposure. Thus the median lethal dose for inhaled A-234 is 0.2 mg (5000 lethal doses in a gram) and is below 0.1 mg for A-230 (10 000 lethal doses in a gram). The agents are reportedly capable of being delivered as a liquid, aerosol or gas via a variety of systems, including artillery shells, bombs, missiles and spraying devices.


Controversy over formulation

Mirzayanov gives somewhat different structures for Novichok agents in his autobiography than those which have been identified by Western experts. The Western formulations suffered from imperfect information, as can be seen in Fig. 1 of Chai ''et al'' in which Mirzayanov describes a family of compounds whereas Western scientists instantiate a particular salt. Mirzyanov makes clear that a large number of compounds were made, and many of the less potent derivatives were reported in the open literature as new organophosphate insecticides, so that the secret chemical weapons program could be disguised as legitimate pesticide research.


Chemistry

According to chemical weapons expert
Jonathan Tucker Jonathan Moss Tucker (born May 31, 1982) is an American actor. He is known for his roles in the films ''The Virgin Suicides'' (1999), '' The Texas Chainsaw Massacre'' (2003), ''Hostage'' (2005), '' In the Valley of Elah'' (2007), '' The Ruins'' ( ...
, the first binary formulation developed under the Foliant programme was used to make Substance 33 ( VR), very similar to the more widely known VX, differing only in the alkyl substituents on its nitrogen and oxygen atoms. "This weapon was given the code name Novichok." A wide range of potential structures have been reported. These all feature the classical
organophosphorus Organophosphorus compounds are organic compounds containing phosphorus. They are used primarily in pest control as an alternative to chlorinated hydrocarbons that persist in the environment. Some organophosphorus compounds are highly effective in ...
core (sometimes with the P=O replaced with P=S or P=Se), which is most commonly depicted as being a
phosphoramidate Phosphoramidates (sometimes also called amidophosphates) are a class of phosphorus compounds structurally related to phosphates (or organophosphates) via the substitution of an OR for a NR2. They are derivatives of phosphoramidic acids O=P(OH)(NR2 ...
or
phosphonate In organic chemistry, phosphonates or phosphonic acids are organophosphorus compounds containing groups (where R = alkyl, aryl, or just hydrogen). Phosphonic acids, typically handled as salts, are generally nonvolatile solids that are poorly ...
, usually fluorinated (cf.
monofluorophosphate Monofluorophosphate is an anion with the formula PO3F2−, which is a phosphate group with one oxygen atom substituted with a fluoride atom. The charge of the ion is −2. The ion resembles sulfate in size, shape and charge, and can thus form comp ...
). The organic groups are subject to more variety; however, a common substituent is
phosgene oxime Phosgene oxime, or CX, is an organic compound with the formula Cl2CNOH. It is a potent chemical weapon, specifically a nettle agent. The compound itself is a colorless solid, but impure samples are often yellowish liquids. It has a strong, disag ...
or analogues thereof. This is a potent chemical weapon in its own right, specifically as a
nettle agent Nettle agents (named after stinging nettles) or urticants are a variety of chemical warfare agents that produce corrosive skin and tissue injury upon contact, resulting in erythema, urticaria, intense itching, and a hive-like rash. Most nettle ...
, and would be expected to increase the harm done by the Novichok agent. Many claimed structures from this group also contain
cross-linking agent In chemistry and biology a cross-link is a bond or a short sequence of bonds that links one polymer chain to another. These links may take the form of covalent bonds or ionic bonds and the polymers can be either synthetic polymers or natural ...
motifs which may covalently bind to the
acetylcholinesterase Acetylcholinesterase (HGNC symbol ACHE; EC 3.1.1.7; systematic name acetylcholine acetylhydrolase), also known as AChE, AChase or acetylhydrolase, is the primary cholinesterase in the body. It is an enzyme Enzymes () are proteins that a ...
enzyme's active site in several places, perhaps explaining the rapid denaturing of the enzyme that is claimed to be characteristic of the Novichok agents. Zoran Radić, a chemist at the University of California, San Diego, performed an ''in silico'' docking study with Mirzayanov's version of the A-232 structure against the active site of the acetylcholinesterase enzyme. The model predicted a tight fit with high binding affinity and formation of a
covalent bond A covalent bond is a chemical bond that involves the sharing of electrons to form electron pairs between atoms. These electron pairs are known as shared pairs or bonding pairs. The stable balance of attractive and repulsive forces between atoms ...
to a
serine Serine (symbol Ser or S) is an α-amino acid that is used in the biosynthesis of proteins. It contains an α-amino group (which is in the protonated − form under biological conditions), a carboxyl group (which is in the deprotonated − form un ...
residue in the active site, with a similar binding mode to established nerve agents such as sarin and soman.


Detection

A procedure of retrospective detection of Novichok type poisons in victim's tissues has been proposed recently Daan Noort,* Alex Fidder, Debora van der Riet-van Oeveren, Ruud Busker, and Marcel J. van der Schans. Verification of Exposure to Novichok Nerve Agents Utilizing a Semitargeted Human Butyrylcholinesterase Nonapeptide Assay. ''Chem. Res. Toxicol.'' 2021, 34, 1926−1932 This method is a modification of the procedure that was developed earlier for identification of sarin poisoning.Fidder, A., Noort, D., Hulst, A. G., De Ruiter, R., Van der Schans, M. J., Benschop, H. P., and Langenberg, J. P. (2002) Retrospective detection of exposure to organophosphorus anti-cholinesterases: mass spectrometric analysis of phosphylated human butyrylcholinesterase. ''Chem. Res. Toxicol.'' 15, 582−590. This method capitalizes on the fact that poisoning by organic phosphonates occurs via phosphonylation of the hydorxy group of serine in the active site of cholinesterases, and that severe poisoning occurs when a major part of these enzymes are inactivated. The concentration of butyryl cholinesterase (HuBuChE) in human plasma is normally about 80 nM. That makes it a good source of adducts that can be subjected to analysis. The procedure consists in three steps (see the Figure A). First, HuBuChE is obtained from the victim's plasma. Second, the enzyme is subjected to pepsin proteolysis. Third, the peptide mixture obtained is subjected to LC-MSMS analysis. If no poisoning took place, the peptide mixture contains a non-modified nonapeptide FGESAGAAS. However, cholinesterases are inactivated due to a chemical reaction with Novichok type nerve agent, the modified nonapeptide is be detected, and its exact (high resolution) mass (along with the mass of the secondary ion produced during collision induced dissociation) allows inambiguous identification of the fact of poisoning and the exact structure of the poison. Thus, the example at Figure A shows the masses of the primary and secondary ions obtained from the plasma of the victim poisoned by A-230. If a victim is poisoned by other Novichok type agents, the masses are different. This method allows identification of poisons at few ppb ratio, but that may be insufficient for reliable detection of the isotopic signature of the adducts, and, therefore an unambiguous identification of the geographic origin of the poison.


Lifetime

According to Vladimir Uglev, who headed a group that worked on the development of the Novichok agents, at least one liquid form of Novichok is very stable with a slow evaporation rate and can remain potent for possibly up to 50 years. Insufficient research has been conducted to fully understand its persistence in various situations in the environment.


Effects and countermeasures

As nerve agents, the Novichok agents belong to the class of
organophosphate In organic chemistry, organophosphates (also known as phosphate esters, or OPEs) are a class of organophosphorus compounds with the general structure , a central phosphate molecule with alkyl or aromatic substituents. They can be considered a ...
acetylcholinesterase inhibitor Acetylcholinesterase inhibitors (AChEIs) also often called cholinesterase inhibitors, inhibit the enzyme acetylcholinesterase from breaking down the neurotransmitter acetylcholine into choline and acetate, thereby increasing both the level and ...
s. These chemical compounds inhibit the enzyme
acetylcholinesterase Acetylcholinesterase (HGNC symbol ACHE; EC 3.1.1.7; systematic name acetylcholine acetylhydrolase), also known as AChE, AChase or acetylhydrolase, is the primary cholinesterase in the body. It is an enzyme Enzymes () are proteins that a ...
, preventing the normal breakdown of the
neurotransmitter A neurotransmitter is a signaling molecule secreted by a neuron to affect another cell across a synapse. The cell receiving the signal, any main body part or target cell, may be another neuron, but could also be a gland or muscle cell. Neuro ...
acetylcholine Acetylcholine (ACh) is an organic chemical that functions in the brain and body of many types of animals (including humans) as a neurotransmitter. Its name is derived from its chemical structure: it is an ester of acetic acid and choline. Part ...
. Acetylcholine concentrations then increase at neuromuscular junctions to cause involuntary contraction of all skeletal muscles (
cholinergic crisis A cholinergic crisis is an over-stimulation at a neuromuscular junction due to an excess of acetylcholine (ACh), as a result of the inactivity of the AChE enzyme, which normally breaks down acetylcholine. Symptoms and diagnosis As a result of c ...
). This then leads to respiratory and cardiac arrest (as the victim's heart and diaphragm muscles no longer function normally) and finally death from heart failure or suffocation as copious fluid secretions fill the victim's lungs. As can be seen with other organophosphate poisonings, Novichok agents may cause lasting nerve damage, resulting in permanent disablement of victims, according to Russian scientists. Their effect on humans was demonstrated by the accidental exposure of Andrei Zheleznyakov, one of the scientists involved in their development, to the residue of an unspecified Novichok agent while working in a Moscow laboratory in May 1987. He was critically injured and took ten days to recover consciousness after the incident. He lost the ability to walk and was treated at a secret clinic in Leningrad for three months afterwards. The agent caused permanent harm, with effects that included "chronic weakness in his arms, a toxic hepatitis that gave rise to
cirrhosis of the liver Cirrhosis, also known as liver cirrhosis or hepatic cirrhosis, and end-stage liver disease, is the impaired liver function caused by the formation of scar tissue known as fibrosis due to damage caused by liver disease. Damage causes tissue repai ...
, epilepsy, spells of severe depression, and an inability to read or concentrate that left him totally disabled and unable to work." He never recovered and, after five years of deteriorating health, died in July 1992. The use of a fast-acting peripheral
anticholinergic Anticholinergics (anticholinergic agents) are substances that block the action of the neurotransmitter called acetylcholine (ACh) at synapses in the central and peripheral nervous system. These agents inhibit the parasympathetic nervous system ...
drug such as
atropine Atropine is a tropane alkaloid and anticholinergic medication used to treat certain types of nerve agent and pesticide poisonings as well as some types of slow heart rate, and to decrease saliva production during surgery. It is typically given i ...
can block the receptors where acetylcholine acts to prevent poisoning (as in the treatment for poisoning by other acetylcholinesterase inhibitors). Atropine, however, is difficult to administer safely, because its effective dose for nerve agent poisoning is close to the dose at which patients suffer severe side effects, such as changes in heart rate and thickening of the bronchial secretions, which fill the lungs of someone suffering nerve agent poisoning so that suctioning of these secretions, and other advanced life support techniques, may be necessary in addition to administration of atropine to treat nerve agent poisoning. In the treatment of nerve agent poisoning, atropine is most often administered along with a Hagedorn oxime such as
pralidoxime Pralidoxime (2-pyridine aldoxime methyl chloride) or 2-PAM, usually as the chloride or iodide salts, belongs to a family of compounds called oximes that bind to organophosphate-inactivated acetylcholinesterase. It is used to treat organophosphat ...
,
obidoxime Obidoxime is a member of the oxime family used to treat nerve gas poisoning. Oximes are drugs known for their ability to reverse the binding of organophosphorus compounds to the enzyme acetylcholinesterase (AChE). AChE is an enzyme that removes ...
,
TMB-4 Trimedoxime bromide (INN), also known as dipyroxime or TMB-4, is an oxime used in the treatment of organophosphate poisoning It is chemically related to pralidoxime Pralidoxime (2-pyridine aldoxime methyl chloride) or 2-PAM, usually as the chl ...
, or
HI-6 Asoxime chloride, or more commonly HI-6, is a Hagedorn oxime used in the treatment of organophosphate poisoning Organophosphate poisoning is poisoning due to organophosphates (OPs). Organophosphates are used as insecticides, medications, and n ...
, which reactivates acetylcholinesterase which has been inactivated by
phosphorylation In chemistry, phosphorylation is the attachment of a phosphate group to a molecule or an ion. This process and its inverse, dephosphorylation, are common in biology and could be driven by natural selection. Text was copied from this source, wh ...
by an organophosphorus nerve agent and relieves the respiratory muscle paralysis caused by some nerve agents. Pralidoxime is not effective in reactivating acetylcholinesterase inhibited by some older nerve agents such as
soman Soman (or GD, EA 1210, Zoman, PFMP, A-255, systematic name: ''O''-pinacolyl methylphosphonofluoridate) is an extremely toxic chemical substance. It is a nerve agent, interfering with normal functioning of the mammalian nervous system by inhibiti ...
or the Novichok nerve agents, described in the literature as being up to eight times more toxic than nerve agent VX. The US Army has funded studies of the use of
galantamine Galantamine is used for the treatment of cognitive decline in mild to moderate Alzheimer's disease and various other memory impairments. It is an alkaloid that has been isolated from the bulbs and flowers of ''Galanthus nivalis'' (Common snowdro ...
along with atropine in the treatment of a number of nerve agents, including soman and the Novichok agents. An unexpected synergistic interaction was seen to occur between galantamine (given between five hours before to thirty minutes after exposure) and atropine in an amount of 6 mg/kg or higher. Increasing the dose of galantamine from 5 to 8 mg/kg decreased the dose of atropine needed to protect experimental animals from the toxicity of soman in dosages 1.5 times the LD50 (lethal dose in half the animals studied). There have been differing claims about the persistence of Novichok and binary precursors in the environment. One view is that it is not affected by normal weather conditions, and may not decompose as quickly as other
organophosphates In organic chemistry, organophosphates (also known as phosphate esters, or OPEs) are a class of organophosphorus compounds with the general structure , a central phosphate molecule with alkyl or aromatic substituents. They can be considered ...
. However, Mirzayanov states that Novichok decomposes within four months.


Instances of usage


Poisoning of Ivan Kivelidi and Zara Ismailova

A Novichok agent was used in 1995 to poison Russian banker , who died three days later in a hospital at the age of 46. The poison was believed to have been applied to Kivelidi's office phone in Moscow. His secretary Zara Ismailova also developed symptoms one month later and then died a day later in a hospital at the age of 35. Kivelidi was the head of the Russian Business Round Table, and had close ties to
Viktor Chernomyrdin Viktor Stepanovich Chernomyrdin (russian: Ви́ктор Степа́нович Черномы́рдин, ; 9 April 19383 November 2010) was a Soviet and Russian politician and businessman. He was the Minister of Gas Industry of the Soviet Unio ...
, who was at that time
Prime Minister of Russia The chairman of the government of the Russian Federation, also informally known as the prime minister, is the nominal head of government of Russia. Although the post dates back to 1905, its current form was established on 12 December 1993 fo ...
.
Russian opposition Opposition to the government of President Vladimir Putin in Russia can be divided between the parliamentary opposition parties in the State Duma and the various non-systemic opposition organizations. While the former are largely viewed as bei ...
–linked historians
Yuri Felshtinsky Yuri Georgievich Felshtinsky (russian: Юрий Георгиевич Фельштинский, born 7 September 1956 in Moscow) is a Russian American historian. Felshtinsky has authored a number of books on Russian history, including ''The Bol ...
and
Vladimir Pribylovsky Vladimir Valerianovich Pribylovsky (russian: Влади́мир Валериа́нович Прибыло́вский, 6 March 195613 January 2016) was a Soviet and Russian political scientist, historian, journalist, human rights activist, and a ...
speculated that the murder became "one of the first in the series of poisonings organised by Russia's security services". The
Russian Ministry of Internal Affairs The Ministry of Internal Affairs of the Russian Federation (MVD; russian: Министерство внутренних дел (МВД), ''Ministerstvo vnutrennikh del'') is the interior ministry of Russia. The MVD is responsible for law enfor ...
analysed the substance and announced that it was "a phosphorus-based military-grade nerve agent" "whose formula was strictly classified". According to Nesterov, the administrative head of Shikhany, he did not know of "a single case of such poison being sold illegally" and noted that the poison "is used by professional spies". Vladimir Khutsishvili, a former business partner of Kivelidi's, was subsequently convicted of the killings. According to ''The Independent'', "A closed trial found that his business partner had obtained the substance via intermediaries from an employee of the State Research Institute of Organic Chemistry and Technology (ГосНИИОХТ / GosNIIOKhT), which was involved in the development of Novichok agents. However, Khutsishvili, who claimed that he was innocent, had not been detained at the time of the trial and freely left the country. He was only arrested in 2006 after he returned to Russia, believing that the ten-year old case was closed. Felshtinsky and Pribylovsky claimed that Russia's security services, which had access to the chemical agent, had framed Khutsishvili for the murder, and that the security services had organised the murder on the orders of a senior Russian state official. Boris Kuznetsov, who represented Khutsishvili and believed in his innocence, blames "rogue intelligence officers". Leonid Rink, an employee of GosNIIOKhT, received a one-year suspended sentence for selling Novichok agents to unnamed buyers "of Chechen ethnicity" soon after the poisoning of Kivelidi and Izmailova.


Poisoning of Sergei and Yulia Skripal

On 12 March 2018, the UK government said that a Novichok agent had been used in an attack in the English city of
Salisbury Salisbury ( ) is a cathedral city in Wiltshire, England with a population of 41,820, at the confluence of the rivers Avon, Nadder and Bourne. The city is approximately from Southampton and from Bath. Salisbury is in the southeast of Wil ...
on 4 March 2018 in an attempt to kill former
GRU The Main Directorate of the General Staff of the Armed Forces of the Russian Federation, rus, Гла́вное управле́ние Генера́льного шта́ба Вооружённых сил Росси́йской Федера́ци ...
officer
Sergei Skripal Sergei Viktorovich Skripal ( rus, Серге́й Ви́кторович Скрипáль, p=sʲɪrˈɡʲej ˈvʲiktərəvʲɪtɕ skrʲɪˈpalʲ; born 23 June 1951) is a former Russian military intelligence officer who acted as a double agent f ...
and his daughter Yulia. British
Prime Minister A prime minister, premier or chief of cabinet is the head of the cabinet and the leader of the ministers in the executive branch of government, often in a parliamentary or semi-presidential system. Under those systems, a prime minister is not ...
Theresa May Theresa Mary May, Lady May (; née Brasier; born 1 October 1956) is a British politician who served as Prime Minister of the United Kingdom and Leader of the Conservative Party from 2016 to 2019. She previously served in David Cameron's cab ...
said in Parliament: "Either this was a direct action by the Russian state against our country, or the Russian government lost control of its potentially catastrophically damaging nerve agent and allowed it to get into the hands of others." On 13 March the BBC asked
Vladimir Putin Vladimir Vladimirovich Putin; (born 7 October 1952) is a Russian politician and former intelligence officer who holds the office of president of Russia. Putin has served continuously as president or prime minister since 1999: as prime min ...
if Russia was "behind the poisoning of" Skripal and he answered "Get to the bottom of it first then we can discuss it" while he delegated a spokesperson to claim that "a circus show in the British parliament" was the upshot.
Boris Johnson Alexander Boris de Pfeffel Johnson (; born 19 June 1964) is a British politician, writer and journalist who served as Prime Minister of the United Kingdom and Leader of the Conservative Party from 2019 to 2022. He previously served as F ...
, the
Foreign Secretary The secretary of state for foreign, Commonwealth and development affairs, known as the foreign secretary, is a minister of the Crown of the Government of the United Kingdom and head of the Foreign, Commonwealth and Development Office. Seen as ...
, refused to shake hands with Russian ambassador Alexander Yakovenko as he expressed "outrage" over the attack. On the next day, the UK expelled 23 Russian diplomats after the Russian government refused to meet the UK's deadline of midnight on 13 March 2018 to give an explanation for the use of the substance. Addressing the
United Nations Security Council The United Nations Security Council (UNSC) is one of the Organs of the United Nations, six principal organs of the United Nations (UN) and is charged with ensuring international security, international peace and security, recommending the admi ...
on 15 March, Vassily Nebenzia, the Russian envoy to the UN, responded to the British allegations by denying that Russia had ever produced or researched the agents, stating: "No scientific research or development under the title novichok were carried out." After the attack, 21 members of the emergency services and public were checked for possible exposure, and three were hospitalised. As of 12 March, one police officer remained in hospital. Five hundred members of the public were advised to decontaminate their possessions to prevent possible long-term exposure, and 180 members of the military and 18 vehicles were deployed to assist with decontamination at locations in and around Salisbury. Up to 38 people in Salisbury have been affected by the agent to an undetermined extent. Daniel Gerstein, a former senior official at the U.S.
Department of Homeland Security The United States Department of Homeland Security (DHS) is the U.S. federal executive department responsible for public security, roughly comparable to the interior or home ministries of other countries. Its stated missions involve anti-terr ...
, said it was possible that Novichok nerve agents had been used before in Britain to assassinate Kremlin targets, but had not been detected: "It's entirely likely that we have seen someone expire from this and not realised it. We realised in this case because they were found unresponsive on a park bench. Had it been a higher dose, maybe they would have died and we would have thought it was natural causes." On 20 March 2018,
Ahmet Üzümcü Ahmet Üzümcü (born August 30, 1951) is a Turkish career diplomat, who previously served as the Director-General of the Organisation for the Prohibition of Chemical Weapons (OPCW). He graduated from Foreign Relations Department of the Faculty ...
, Director-General of the OPCW, said that it would take "another two to three weeks to finalise the analysis" of samples taken from the poisoning of Skripal. On 3 April 2018, the
Defence Science and Technology Laboratory The Defence Science and Technology Laboratory (Dstl) is an executive agency of the Ministry of Defence (United Kingdom), Ministry of Defence of the United Kingdom. Its stated purpose is "to maximise the impact of science and technology for the ...
announced that it was "completely confident" that the agent used was Novichok, although they still did not know the "precise source" of the agent. Experts said that their findings did not challenge the conclusions by UK government: "We provided that information to the Government who have then used a number of other sources to come to the conclusions that they have." On 12 April 2018 the OPCW announced that their investigations agreed with the conclusions made by the UK about the identity of the chemical used. By September 2018 two Russian "tourists", " Alexander Petrov" and " Ruslan Boshirov", had been identified as suspects. They told
Margarita Simonyan Margarita Simonovna Simonyan (russian: link=no, Маргарита Симоновна Симоньян; born 6 April 1980) is a Russian journalist. She is the editor-in-chief of the Russian state-controlled media organisations RT (formerly R ...
, the chief editor of RT television, in an interview that they both worked in the sports nutrition business and that "Those are our real names.. We're afraid to go out, we fear for ourselves, our lives and lives of our loved ones." The
Crown Prosecution Service The Crown Prosecution Service (CPS) is the principal public agency for conducting criminal prosecutions in England and Wales. It is headed by the Director of Public Prosecutions. The main responsibilities of the CPS are to provide legal adv ...
announced enough evidence was obtained by that date "to convict the two men" of the attack, although it did not apply to Russia "for their extradition because Russia does not extradite its own nationals... A European Arrest Warrant has been obtained in case they travel to the EU." In February 2019, the
Bellingcat Bellingcat (stylised as bellngcat) is a Netherlands-based investigative journalism group that specialises in fact-checking and open-source intelligence (OSINT). It was founded by British journalist and former blogger Eliot Higgins in July 2014 ...
website published precise allegations that identified
GRU The Main Directorate of the General Staff of the Armed Forces of the Russian Federation, rus, Гла́вное управле́ние Генера́льного шта́ба Вооружённых сил Росси́йской Федера́ци ...
Major Denis Vyacheslavovich Sergeev as a man who travelled in March 2018 to London under the false identity of Sergei Fedotov. It is claimed with detailed photograph evidence, and phone, travel, passport, and motoring database records that GRU Colonels Alexander Mishkin and
Anatoly Chepiga Anatoly Vladimirovich Chepiga (russian: Анатолий Владимирович Чепига, born 5 April 1979) is a Colonel (Eastern Europe), colonel in the Russian General Staff's Main Directorate (also known as GRU), the military intell ...
assumed the identities of Petrov and Boshirov, placed the poison on Skripal's doorknob. On 28 June 2019 it was reported that Sergeyev received instructions from his GRU superior by cell phone on more than ten occasions during his UK visits.


Poisoning of Charlie Rowley and Dawn Sturgess

On 30 June 2018, Charlie Rowley and Dawn Sturgess were found unconscious at a house in
Amesbury Amesbury () is a town and civil parish in Wiltshire, England. It is known for the prehistoric monument of Stonehenge which is within the parish. The town is claimed to be the oldest occupied settlement in Great Britain, having been first settle ...
,
Wiltshire Wiltshire (; abbreviated Wilts) is a historic and ceremonial county in South West England with an area of . It is landlocked and borders the counties of Dorset to the southwest, Somerset to the west, Hampshire to the southeast, Gloucestershire ...
, about eight miles from the Salisbury poisoning site. On 4 July 2018, police said that the pair had been poisoned with the same nerve agent as ex-Russian spy Sergei Skripal. On 8 July 2018, Sturgess died as a result of the poisoning. Rowley regained consciousness and began recovering in hospital. He told his brother Matthew the nerve agent had been in a small perfume or aftershave bottle, which they had found in a park about nine days before spraying themselves with it. The police later closed and fingertip-searched Queen Elizabeth Gardens in Salisbury.


Poisoning of Emilian Gebrev

In the aftermath of the Skripal poisoning, investigative journalists were able to track some of the people involved also in Bulgaria. This is how another suspected poisoning case dating back to April 2015 during their stay in the country was linked to the Novichok nerve agent. The victim was the Bulgarian arms dealer Emilian Gebrev, who shares two hypotheses why he might have been attacked: The first one links to the fact that his arms manufacturing company Dunarit exports defense equipment to Ukraine. The other one relates to an attempt by an offshore company to take over Dunarit. The takeover attempt was ultimately linked to the influential Bulgarian politician and oligarch
Delyan Peevski Delyan Slavchev Peevski ( bg, Делян Славчев Пеевски ) (born 27 July 1980) is a Bulgarian politician, oligarch, entrepreneur and media mogul. He served as MP from the parliamentary group of the DPS in the 41st, 42nd, 43rd an ...
who has historically been funded by Russia's state-owned
VTB Bank VTB Bank (; formerly known as ''Vneshtorgbank'', , lit. 'International Trade Bank') is a Russian majority state-owned bank headquartered in various federal districts of Russia; its legal address is registered in Saint Petersburg, St. Peter ...
.


Poisoning of Alexei Navalny

On 20 August 2020, Russian opposition leader
Alexei Navalny Alexei Anatolievich Navalny ( rus, links=no, Алексей Анатольевич Навальный, , ɐlʲɪkˈsʲej ɐnɐˈtolʲjɪvʲɪtɕ nɐˈvalʲnɨj; born 4 June 1976) is a Opposition to Vladimir Putin in Russia, Russian opposition ...
fell ill during a flight from
Tomsk Tomsk ( rus, Томск, p=tomsk, sty, Түң-тора) is a city and the administrative center of Tomsk Oblast in Russia, located on the Tom River. Population: Founded in 1604, Tomsk is one of the oldest cities in Siberia. The city is a not ...
to
Moscow Moscow ( , US chiefly ; rus, links=no, Москва, r=Moskva, p=mɐskˈva, a=Москва.ogg) is the capital and largest city of Russia. The city stands on the Moskva River in Central Russia, with a population estimated at 13.0 million ...
. The plane made an emergency landing in
Omsk Omsk (; rus, Омск, p=omsk) is the administrative center and largest city of Omsk Oblast, Russia. It is situated in southwestern Siberia, and has a population of over 1.1 million. Omsk is the third largest city in Siberia after Novosibirsk ...
, where Navalny was hospitalized and put in a medically induced coma. His family suspected his illness was caused by a poison put into a cup of tea he drank before the flight. He was evacuated to the
Charité The Charité – Universitätsmedizin Berlin (Charité – Berlin University of Medicine) is one of Europe's largest university hospitals, affiliated with Humboldt University and Free University Berlin. With numerous Collaborative Research Cen ...
hospital in
Berlin Berlin ( , ) is the capital and largest city of Germany by both area and population. Its 3.7 million inhabitants make it the European Union's most populous city, according to population within city limits. One of Germany's sixteen constitue ...
, Germany, the following day. On 2 September, the German government said that it had "unequivocal evidence" that Navalny was poisoned by a Novichok agent after tests at a German military lab and had called on the Russian government for an explanation, with labs in France and Sweden corroborating the findings. On 4 September, the
North Atlantic Council The North Atlantic Council (NAC) is the principal political decision-making body of the North Atlantic Treaty Organization (NATO), consisting of permanent representatives of its member countries. It was established by Article 9 of the North A ...
was briefed by the German representative on the "appalling assassination attempt on" Navalny. In a post-meeting press conference, Secretary-General
Jens Stoltenberg Jens Stoltenberg (born 16 March 1959) is a Norwegian politician who has been serving as the 13th secretary general of NATO since 2014. A member of the Norwegian Labour Party, he previously served as the 34th prime minister of Norway from 2000 to ...
said that NATO allies "agree that Russia has serious questions it must answer", that the OPCW needed to conduct an impartial investigation, that "those responsible for this attack must be brought to justice" and called on Russia to "provide complete disclosure of the Novichok programme to the OPCW." Navalny has been out of his coma since 7 September. On 6 October, the OPCW confirmed the presence of a
cholinesterase inhibitor Cholinesterase inhibitors (ChEIs), also known as anti-cholinesterase, are chemicals that prevent the breakdown of the neurotransmitter acetylcholine or butyrylcholine. This increases the amount of the acetylcholine or butyrylcholine in the syn ...
from the Novichok group in Navalny's blood and urine samples. At the same time, the OPCW report clarified that Navalny was poisoned with a new type of Novichok, which was not included in the list of controlled chemicals of the
Chemical Weapons Convention The Chemical Weapons Convention (CWC), officially the Convention on the Prohibition of the Development, Production, Stockpiling and Use of Chemical Weapons and on their Destruction, is an arms control treaty administered by the Organisation for ...
.


See also

*
Poison laboratory of the Soviet secret services The poison laboratory of the Soviet secret services, alternatively known as Laboratory 1, Laboratory 12, and Kamera (which means "The Cell" in Russian), was a covert research-and-development facility of the Soviet secret police agencies. Th ...
*
Russia and weapons of mass destruction Russia (, , ), or the Russian Federation, is a transcontinental country spanning Eastern Europe and Northern Asia. It is the largest country in the world, with its internationally recognised territory covering , and encompassing one-eigh ...


List of Novichok agents

*
A-230 A-230 is an organophosphate nerve agent. It was developed in the Soviet Union under the FOLIANT program and is one of the group of compounds referred to as Novichok agents that were revealed by Vil Mirzayanov. A-230 is possibly the most potent ner ...
*
A-232 A-232 is an organophosphate nerve agent. It was developed in the Soviet Union under the FOLIANT program and is one of the group of compounds referred to as Novichok agents that were revealed by Vil Mirzayanov. A-232 is reportedly slightly less pot ...
* A-234 *
A-242 A-242 is an organophosphate nerve agent. It was developed in the Soviet Union under the FOLIANT program and is one of the group of compounds referred to as Novichok agents that were revealed by Vil Mirzayanov. Mirzayanov gives little specific info ...
*
A-262 A-262 is an organophosphate nerve agent. It was developed in the Soviet Union under the FOLIANT program and is one of the group of compounds referred to as Novichok agents that were revealed by Vil Mirzayanov. Mirzayanov gives little specific infor ...
(Novichok-7) *
C01-A035 C01-A035 is a Novichok agent. It is the methyl phosphorofluoridate ester of phosgene oxime Phosgene oxime, or CX, is an organic compound with the formula Cl2CNOH. It is a potent chemical weapon, specifically a nettle agent. The compound its ...
*
C01-A039 C01-A039 is a Novichok agent. It is the ethyl phosphorofluoridate ester of phosgene oxime. See also *C01-A035 C01-A035 is a Novichok agent. It is the methyl phosphorofluoridate ester of phosgene oxime Phosgene oxime, or CX, is an organic ...
* C01-A042


References


Notes


Citations


Bibliography

* * * * * * * * * * * * * * *


Further reading

*


External links

* * {{Neurotoxins Acetylcholinesterase inhibitors Cold War weapons of the Soviet Union Nerve agents Organophosphates Science and technology in the Soviet Union Soviet chemical weapons program Soviet inventions