Nicoletta Sacchi (born August 20, 1949) is an Italian professor of

oncology Oncology is a branch of medicine that deals with the study, treatment, diagnosis and prevention of cancer. A medical professional who practices oncology is an ''oncologist''. The name's etymological origin is the Greek word ὄγκος (''ó ...

at the

Roswell Park Comprehensive Cancer Center Roswell Park Comprehensive Cancer Center is a cancer research and treatment center located in Buffalo, New York. Founded by surgeon Roswell Park in 1898, the center was the first in the United States to specifically focus on cancer research. The ...

. She is the co-discoverer of the

acid guanidinium thiocyanate-phenol-chloroform extraction Acid guanidinium thiocyanate-phenol-chloroform extraction (abbreviated AGPC) is a liquid–liquid extraction technique in biochemistry. It is widely used in molecular biology for isolating RNA (as well as DNA and protein in some cases). This metho ...

method to extract RNA from biological samples with

Trizol TRIzol is a widely used chemical solution used in the extraction of DNA, RNA, and proteins from cells. The solution was initially used and published by Piotr Chomczyński and Nicoletta Sacchi in 1987. TRIzol is the brand name of guanidinium thi ...

. As a consequence of this groundbreaking discovery she is now considered the most

cited A citation is a reference to a source. More precisely, a citation is an abbreviated alphanumeric expression embedded in the body of an intellectual work that denotes an entry in the bibliographic references section of the work for the purpose of ...

woman scientist in the world.

Career

Sacchi was born in

Milan Milan ( , , Lombard: ; it, Milano ) is a city in northern Italy, capital of Lombardy, and the second-most populous city proper in Italy after Rome. The city proper has a population of about 1.4 million, while its metropolitan city h ...

and studied for her PhD at the

University of Milan The University of Milan ( it, Università degli Studi di Milano; la, Universitas Studiorum Mediolanensis), known colloquially as UniMi or Statale, is a public research university in Milan, Italy. It is one of the largest universities in Europe ...

in 1972, investigating the

genetics Genetics is the study of genes, genetic variation, and heredity in organisms.Hartl D, Jones E (2005) It is an important branch in biology because heredity is vital to organisms' evolution. Gregor Mendel, a Moravian Augustinian friar wor ...

yeast Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom. The first yeast originated hundreds of millions of years ago, and at least 1,500 species are currently recognized. They are estimated to constitut ...

. She remained within the fields of genetics for her postdoctoral training at the same University and

Erasmus University Erasmus University Rotterdam (abbreviated as ''EUR'', nl, Erasmus Universiteit Rotterdam ) is a public research university located in Rotterdam, Netherlands. The university is named after Desiderius Erasmus Roterodamus, a 15th-century humanist ...

Rotterdam Rotterdam ( , , , lit. ''The Dam on the River Rotte'') is the second largest city and municipality in the Netherlands. It is in the province of South Holland, part of the North Sea mouth of the Rhine–Meuse–Scheldt delta, via the ''"N ...

. Sacchi worked on the generation of antibody repertoires by the

TdT Terminal deoxynucleotidyl transferase (TdT), also known as DNA nucleotidylexotransferase (DNTT) or terminal transferase, is a specialized DNA polymerase expressed in immature, pre-B, pre-T lymphoid cells, and acute lymphoblastic leukemia/lymphom ...

DNA polymerase A DNA polymerase is a member of a family of enzymes that catalyze the synthesis of DNA molecules from nucleoside triphosphates, the molecular precursors of DNA. These enzymes are essential for DNA replication and usually work in groups to create ...

in the lab of

Georges Köhler Georges may refer to: Places * Georges River, New South Wales, Australia * Georges Quay (Dublin) *Georges Township, Fayette County, Pennsylvania Other uses *Georges (name) * ''Georges'' (novel), a novel by Alexandre Dumas * "Georges" (song), a 19 ...

at the

Basel Institute for Immunology The Basel Institute for Immunology (BII) was founded in 1969 as a basic research institute in immunology located at 487 Grenzacherstrasse, Basel, Switzerland on the Rhine River down the street from the main Hoffmann-La Roche campus near the Swiss-G ...

. She moved to the US in 1982 and switched to cancer research, focussing on the analysis of the genetics of patient samples at

National Cancer Institute The National Cancer Institute (NCI) coordinates the United States National Cancer Program and is part of the National Institutes of Health (NIH), which is one of eleven agencies that are part of the U.S. Department of Health and Human Services. ...

. This required a high yield technique to extract RNA from scarce paediatric

leukemia Leukemia ( also spelled leukaemia and pronounced ) is a group of blood cancers that usually begin in the bone marrow and result in high numbers of abnormal blood cells. These blood cells are not fully developed and are called ''blasts'' or ' ...

samples, so her and colleague Piotr Chomczynski developed a new method to extract the nucleic acid. Since the publication of the technique in 1987 it has become ubiquitous in biological research, earning the paper the accolade of being one of the most cited in history. Sacchi went back to the University of Milan in 1991 and was promoted to professor. Unsatisfied with research in Italy she spent time back in the US as a visiting professor at the Sydney Kimmel Comprehensive Cancer Center at

Johns Hopkins University Johns Hopkins University (Johns Hopkins, Hopkins, or JHU) is a private university, private research university in Baltimore, Maryland. Founded in 1876, Johns Hopkins is the oldest research university in the United States and in the western hem ...

. In 2003 she joined the Roswell Park Comprehensive Cancer Center where she continues to study the role of genetics and the

epigenome An epigenome consists of a record of the chemical changes to the DNA and histone proteins of an organism; these changes can be passed down to an organism's offspring via transgenerational stranded epigenetic inheritance. Changes to the epigenome ...

on cancer development. Sacchi has previously criticised the culture of science in her home nation of Italy, describing the system as “fairly corrupt” and ignorant of the achievements of female scientists. She believes that this has led to brain drain of trained researchers.

Accolades

In 2001 Sacchi was proclaimed a “Highly Cited Researcher in Biology and Biochemistry” by the

Institute for Scientific Information The Institute for Scientific Information (ISI) was an academic publishing service, founded by Eugene Garfield in Philadelphia in 1956. ISI offered scientometric and bibliographic database services. Its specialty was citation indexing and analysis, ...

Philadelphia Philadelphia, often called Philly, is the largest city in the Commonwealth of Pennsylvania, the sixth-largest city in the U.S., the second-largest city in both the Northeast megalopolis and Mid-Atlantic regions after New York City. Sinc ...

. In 2007 the Italian

President President most commonly refers to: *President (corporate title) *President (education), a leader of a college or university *President (government title) President may also refer to: Automobiles * Nissan President, a 1966–2010 Japanese ful ...

awarded Sacchi with the Commander of Merit.

References

{{DEFAULTSORT:Sacchi, Nicoletta Italian expatriates in the United States Expatriate academics in the United States University of Milan alumni Italian expatriates in Switzerland Italian expatriates in Germany Living people Italian oncologists Italian women scientists 1949 births