The mitochondrial permeability transition pore (mPTP or MPTP; also referred to as PTP, mTP or MTP) is a protein
that is formed in the inner membrane of the mitochondria
under certain pathological conditions such as traumatic brain injury
. Opening allows increase in the permeability of the mitochondria
l membranes to molecule
s of less than 1500 Dalton
s in molecular weight. Induction of the permeability transition pore, mitochondrial membrane permeability transition (mPT or MPT), can lead to mitochondrial swelling and cell death
depending on the particular biological setting.
Roles in pathology
The MPTP was originally discovered by Haworth and Hunter in 1979 and has been found to be involved in neurodegeneration
, hepatotoxicity from Reye-related agents, cardiac necrosis and nervous and muscular dystrophies among other deleterious events inducing cell damage and death.
MPT is one of the major causes of cell death in a variety of conditions. For example, it is key in neuronal cell death in excitotoxicity
, in which overactivation of glutamate receptor
s causes excessive calcium entry into the cell
MPT also appears to play a key role in damage caused by ischemia
, as occurs in a heart attack
However, research has shown that the MPT pore remains closed during ischemia, but opens once the tissues are reperfused
with blood after the ischemic period, playing a role in reperfusion injury
MPT is also thought to underlie the cell death induced by Reye's syndrome
, since chemicals that can cause the syndrome, like salicylate
, cause MPT.
MPT may also play a role in mitochondrial autophagy
Cells exposed to toxic amounts of Ca2+ ionophore
s also undergo MPT and death by necrosis.
While the MPT modulation has been widely studied, little is known about its structure
. Initial experiments by Szabó and Zoratti proposed the MPT may comprise Voltage Dependent Anion Channel (VDAC) molecules. Nevertheless, this hypothesis was shown to be incorrect as VDAC−/−
mitochondria were still capable to undergo MPT. Further hypothesis by Halestrap's group convincingly suggested the MPT was formed by the inner membrane Adenine Nucleotide Translocase (ANT), but genetic ablation of such protein still led to MPT onset. Thus, the only MPTP components identified so far are the TSPO
(previously known as the peripheral benzodiazepine receptor) located in the mitochondrial outer membrane and cyclophilin
-D in the mitochondrial matrix
. Mice lacking the gene for cyclophilin-D develop normally, but their cells do not undergo Cyclosporin A-sensitive MPT, and they are resistant to necrotic death from ischemia or overload of Ca2+
or free radicals.
However, these cells do die in response to stimuli that kill cells through apoptosis, suggesting that MPT does not control cell death by apoptosis.
Agents that transiently block MPT include the immune
suppressant cyclosporin A
(CsA); N-methyl-Val-4-cyclosporin A
(MeValCsA), a non-immunosuppressant
derivative of CsA; another non-immunosuppressive agent, NIM811
, 2-aminoethoxydiphenyl borate (2-APB), bongkrekic acid
(also known as Debio-025). TRO40303 is a newly synthetitised MPT blocker developed by Trophos
company and currently is in Phase I clinical trial
Factors in MPT induction
Various factors enhance the likelihood of MPTP opening. In some mitochondria, such as those in the central nervous system
, high levels of Ca2+
within mitochondria can cause the MPT pore to open.
This is possibly because Ca2+
binds to and activates Ca2+
binding sites on the matrix side of the MPTP.
MPT induction is also due to the dissipation of the difference in voltage
across the inner mitochondrial membrane (known as transmembrane potential, or Δψ).
In neurons and astrocytes, the contribution of membrane potential to MPT induction is complex, see.
The presence of free radical
s, another result of excessive intracellular calcium concentration
s, can also cause the MPT pore to open.
Other factors that increase the likelihood that the MPTP will be induced include the presence of certain fatty acids,
and inorganic phosphate.
However, these factors cannot open the pore without Ca2+
, though at high enough concentrations, Ca2+
alone can induce MPT.
Stress in the endoplasmic reticulum
can be a factor in triggering MPT.
Conditions that cause the pore to close or remain closed include acid
high concentrations of ADP
high concentrations of ATP
and high concentrations of NADH
. Divalent cation
s like Mg2+
also inhibit MPT, because they can compete with Ca2+
for the Ca2+
binding sites on the matrix and/or cytoplasmic side of the MPTP.
Multiple studies have found the MPT to be a key factor in the damage to neurons caused by excitotoxicity
The induction of MPT, which increases mitochondrial membrane permeability, causes mitochondria to become further depolarized, meaning that Δψ is abolished. When Δψ is lost, proton
s and some molecules are able to flow across the outer mitochondrial membrane uninhibited.
Loss of Δψ interferes with the production of adenosine triphosphate
(ATP), the cell's main source of energy, because mitochondria must have an electrochemical gradient
to provide the driving force for ATP production.
In cell damage resulting from conditions such as neurodegenerative disease
s and head injury
, opening of the mitochondrial permeability transition pore can greatly reduce ATP production, and can cause ATP synthase
to begin hydrolysing
, rather than producing, ATP.
This produces an energy deficit in the cell, just when it most needs ATP to fuel activity of ion pump
s such as the Na+/Ca2+ exchanger
, which must be activated more than under normal conditions in order to rid the cell of excess calcium.
MPT also allows Ca2+
to leave the mitochondrion, which can place further stress on nearby mitochondria, and which can activate harmful calcium-dependent protease
s such as calpain
Reactive oxygen species
(ROS) are also produced as a result of opening the MPT pore. MPT can allow antioxidant
molecules such as glutathione
to exit mitochondria, reducing the organelles' ability to neutralize ROS. In addition, the electron transport chain
(ETC) may produce more free radicals due to loss of components of the ETC, such as cytochrome ''c''
, through the MPTP.
Loss of ETC components can lead to escape of electrons from the chain, which can then reduce molecules and form free radicals.
MPT causes mitochondria to become permeable to molecules smaller than 1.5 kDa, which, once inside, draw water in by increasing the organelle's osmolar load
This event may lead mitochondria to swell and may cause the outer membrane to rupture, releasing cytochrome ''c''.
Cytochrome ''c'' can in turn cause the cell to go through apoptosis
("commit suicide") by activating pro-apoptotic factors. Other researchers contend that it is not mitochondrial membrane rupture that leads to cytochrome ''c'' release, but rather another mechanism, such as translocation of the molecule through channels in the outer membrane, which does not involve the MPTP.
Much research has found that the fate of the cell after an insult depends on the extent of MPT. If MPT occurs to only a slight extent, the cell may recover, whereas if it occurs more it may undergo apoptosis. If it occurs to an even larger degree the cell is likely to undergo necrotic cell death
Possible evolutionary purpose
Although the MPTP has been studied mainly in mitochondria from mammalian sources, mitochondria from diverse species also undergo a similar transition. While its occurrence can be easily detected, its purpose still remains elusive. Some have speculated that the regulated opening of the MPT pore may minimize cell injury by causing ROS-producing mitochondria to undergo selective lysosome-dependent mitophagy during nutrient starvation conditions. Under severe stress/pathologic conditions, MPTP opening would trigger injured cell death mainly through necrosis.
[Haworth RA and Hunter DR. 2001. Ca2+-induced transition in mitochondria: A cellular catastrophe? Chapter 6 In ''Mitochondria in pathogenesis''. Lemasters JJ and Nieminen AL, eds. Kluwer Academic/Plenum Publishers. New York. Pages 115 - 124.]
There is controversy about the question of whether the MPTP is able to exist in a harmless, "low-conductance" state. This low-conductance state would not induce MPT
and would allow certain molecules and ions to cross the mitochondrial membranes. The low-conductance state may allow small ions like Ca2+
to leave mitochondria quickly, in order to aid in the cycling of Ca2+
in healthy cells.
If this is the case, MPT may be a harmful side effect of abnormal activity of a usually beneficial MPTP.
MPTP has been detected in mitochondria from plants, yeasts, such as ''Saccharomyces cerevisiae'', birds, such as guinea fowl and primitive vertebrates such as the Baltic lamprey
. While the permeability transition is evident in mitochondria from these sources, its sensitivity to its classic modulators may differ when compared with mammalian mitochondria. Nevertheless, CsA-insensitive MPTP can be triggered in mammalian mitochondria given appropriate experimental conditions strongly suggesting this event may be a conserved characteristic throughout the eukaryotic domain.
*NMDA receptor antagonist
External linksMitochondrial permeability transition pore: an enigmatic gatekeeper
(2012) NHS&T, Vol 1(3):47-51
from Celldeath.de. Accessed January 1, 2007.