KIAA1841 is a

gene In biology, the word gene (from , ; "...Wilhelm Johannsen coined the word gene to describe the Mendelian units of heredity..." meaning ''generation'' or ''birth'' or ''gender'') can have several different meanings. The Mendelian gene is a ba ...

in humans that encodes a

protein Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, respo ...

known as KIAA1841 (uncharacterized protein KIAA1841). KIAA1841 is targeted for the

nucleus Nucleus ( : nuclei) is a Latin word for the seed inside a fruit. It most often refers to: *Atomic nucleus, the very dense central region of an atom *Cell nucleus, a central organelle of a eukaryotic cell, containing most of the cell's DNA Nucle ...

and it predicted to play a role in regulating

transcription Transcription refers to the process of converting sounds (voice, music etc.) into letters or musical notes, or producing a copy of something in another medium, including: Genetics * Transcription (biology), the copying of DNA into RNA, the fir ...

Gene

Location

KIAA1841 is located on the long arm of

chromosome 2 Chromosome 2 is one of the twenty-three pairs of chromosomes in humans. People normally have two copies of this chromosome. Chromosome 2 is the second-largest human chromosome, spanning more than 242 million base pairs and representing almost e ...

(2q14), starting at 61297486 and ending at 61349294. The KIAA1841 gene spans 52809 base pairs and is orientated on the ++ strand. The coding region is made up of 4292 base pairs and the protein sequence of 718

amino acids Amino acids are organic compounds that contain both amino and carboxylic acid functional groups. Although hundreds of amino acids exist in nature, by far the most important are the alpha-amino acids, which comprise proteins. Only 22 alpha am ...

Gene neighborhood

Genes

PEX13 Peroxisomal membrane protein PEX13 is a protein that in humans is encoded by the ''PEX13'' gene. It located on chromosome 2 next to KIAA1841 Interactions PEX13 has been shown to interact with PEX14, PEX5 and PEX19 Peroxisomal biogenesis fact ...

and C2orf74 neighbor KIAA1841 on chromosome 2.

Expression

KIAA1841 is highly expressed in reproductive structures and nervous tissue. These include the brain, prostate, cervix, ear and nervous tissue. It is intermediately expressed in the lungs and spinal cord. KIAA1841 is expressed at low levels in a wide range of tissues throughout the human body.

Transcript variants

In humans, the KIAA1841 gene produces 18 alternatively spliced transcript variants as well as 3 unspliced. From the 18 spliced variants 4 form a protein product. The main transcript in humans is transcript ID ENST00000402291, or OTTHUMT00000325477.

Homology

Paralogs

There are no

paralogs Sequence homology is the biological homology between DNA, RNA, or protein sequences, defined in terms of shared ancestry in the evolutionary history of life. Two segments of DNA can have shared ancestry because of three phenomena: either a spec ...

of KIAA1841

Orthologs

Below is a table of a variety of

orthologs Sequence homology is the biological homology between DNA, RNA, or protein sequences, defined in terms of shared ancestry in the evolutionary history of life. Two segments of DNA can have shared ancestry because of three phenomena: either a spec ...

of the human KIAA1841. The table include closely, intermediately and distantly related orthologs. Orthologs of the human protein KIAA1841 are listed above in descending order or date of divergence and then ascending order of percent identity. KIAA1841 is highly conserved throughout all orthologs, this is demonstrated with a 40% identity in the least similar ortholog. KAA1841 has evolved slowly and evenly over time.

Homologous domains

The domain of unknown function 3342 is conserved in all orthologs. It is the highest conserved region of the protein. Conservation of this domain was traced all the way back to a fungus called ''

Batrachochytrium dendrobatidis ''Batrachochytrium dendrobatidis'' ( ), also known as ''Bd'' or the amphibian chytrid fungus, is a fungus that causes the disease chytridiomycosis in amphibians. Since its discovery in 1998 by Lee Berger, the disease devastated amphibian popul ...

'', which diverged 1216 million years ago from humans.

Protein

General properties

The

molecular weight A molecule is a group of two or more atoms held together by attractive forces known as chemical bonds; depending on context, the term may or may not include ions which satisfy this criterion. In quantum physics, organic chemistry, and bioch ...

of KIAA1841 is 82 kiloDaltons. The

isoelectric point The isoelectric point (pI, pH(I), IEP), is the pH at which a molecule carries no net electrical charge or is electrically neutral in the statistical mean. The standard nomenclature to represent the isoelectric point is pH(I). However, pI is also u ...

is 6.5. The protein sequence is not rich or low in any amino acids. There are two stretches of

non-polar In chemistry, polarity is a separation of electric charge leading to a molecule or its chemical groups having an electric dipole moment, with a negatively charged end and a positively charged end. Polar molecules must contain one or more pola ...

regions, which are capable of being

transmembrane A transmembrane protein (TP) is a type of integral membrane protein that spans the entirety of the cell membrane. Many transmembrane proteins function as gateways to permit the transport of specific substances across the membrane. They frequentl ...

regions. There is a stretch of 21 0’s from 254-275 and a stretch of 24 0’s from 420–444.1 The DUF3342 domain stretches from 147–449.

Composition

There is an even distribution of amino acids comprising KIAA1841. The percent composition of each amino acid is fairly consistent throughout the orthologs of the protein. The most distant ortholog displays the most variance in amino acid composition. There is a higher percent composition of

alanine Alanine (symbol Ala or A), or α-alanine, is an α-amino acid that is used in the biosynthesis of proteins. It contains an amine group and a carboxylic acid group, both attached to the central carbon atom which also carries a methyl group side c ...

histidine Histidine (symbol His or H) is an essential amino acid that is used in the biosynthesis of proteins. It contains an α-amino group (which is in the protonated –NH3+ form under biological conditions), a carboxylic acid group (which is in the de ...

and

leucine Leucine (symbol Leu or L) is an essential amino acid that is used in the biosynthesis of proteins. Leucine is an α-amino acid, meaning it contains an α-amino group (which is in the protonated −NH3+ form under biological conditions), an α- ca ...

and a lower composition of

lysine Lysine (symbol Lys or K) is an α-amino acid that is a precursor to many proteins. It contains an α-amino group (which is in the protonated form under biological conditions), an α-carboxylic acid group (which is in the deprotonated −C ...

. The protein sequence of KIAA1841 is not rich or low in any amino acids. The same is true in ''

Mus musculus Mus or MUS may refer to: Abbreviations * MUS, the NATO country code for Mauritius * MUS, the IATA airport code for Minami Torishima Airport * MUS, abbreviation for the Centre for Modern Urban Studies on Campus The Hague, Leiden University, Neth ...

'', ''

Danio rerio The zebrafish (''Danio rerio'') is a freshwater fish belonging to the minnow family (Cyprinidae) of the order Cypriniformes. Native to South Asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio (and thus often ...

'', ''

Drosophila melanogaster ''Drosophila melanogaster'' is a species of fly (the taxonomic order Diptera) in the family Drosophilidae. The species is often referred to as the fruit fly or lesser fruit fly, or less commonly the "vinegar fly" or "pomace fly". Starting with Ch ...

'' but not true for the most distantly related. ''

'' is rich is histidine. Humans and closely related orthologs are composed of 2.2% to 3.8% histidine compared to 5% in ''Batrachochytrium dendrobatidis''.

Domains

The DUF3342 domain stretches from 147-449 on KIAA1841 and has a molecular weight of 35.7 kdal. The DUF domain is low in G (2%) and rich is C (6.3%). Both of the non-polar stretches in the protein are located within the DUF domain. One at the beginning and one at the end. The domain (DUF3342) of unknown function is a part of the pfam11822 family. This family of proteins has yet to be functionally characterized and it is found in bacteria. This domain is usually between 170 amino acids and 303 amino acids in length. The N terminal half of this protein family is a BTB-like domain. BTB domains multifunctional protein-protein interaction motif that is involved in a number of different cellular functions, including roles in regulating transcription, cytoskeleton dynamics, gating and assembly of ion channels and is involved with ubiquitination of proteins. BTB domain structures are highly conversed and are found on proteins that only have one or two other types of domains.

Post-translational modifications Post-translational modification (PTM) is the covalent and generally enzymatic modification of proteins following protein biosynthesis. This process occurs in the endoplasmic reticulum and the golgi apparatus. Proteins are synthesized by ribosomes ...

KIAA1841 is highly phosphorylated post modification. There are 37 predicted phosphorylated sites. There is one leucine-rich nuclear export signal toward the end of the protein. There is one sulfated tyrosine, which strengthens protein-protein interactions. Two motifs with high probability of post translational modification

sumoylation In molecular biology, SUMO (Small Ubiquitin-like Modifier) proteins are a family of small proteins that are covalently attached to and detached from other proteins in cells to modify their function. This process is called SUMOylation (sometimes w ...

sites were found. Sumoylation sites are involved in a number of cellular processes, including nuclear-cytosolic transport, transcriptional regulation and protein stability.

Secondary structure Protein secondary structure is the three dimensional conformational isomerism, form of ''local segments'' of proteins. The two most common Protein structure#Secondary structure, secondary structural elements are alpha helix, alpha helices and beta ...

KIAA1841 is primarily composed of

alpha helices The alpha helix (α-helix) is a common motif in the secondary structure of proteins and is a right hand-helix conformation in which every backbone N−H group hydrogen bonds to the backbone C=O group of the amino acid located four residues ear ...

and

beta sheets The beta sheet, (β-sheet) (also β-pleated sheet) is a common motif of the regular protein secondary structure. Beta sheets consist of beta strands (β-strands) connected laterally by at least two or three backbone hydrogen bonds, forming a gen ...

. Alpha helices comprise the majority of the protein, this is true for the DUF domain and both terminuses. The DUF domain has slightly less beta sheets compared to the protein as a whole and the

C terminus The C-terminus (also known as the carboxyl-terminus, carboxy-terminus, C-terminal tail, C-terminal end, or COOH-terminus) is the end of an amino acid chain (protein or polypeptide), terminated by a free carboxyl group (-COOH). When the protein is ...

has an even smaller amount of beta sheets comprising its secondary structure.

Subcellular localization

Protein KIAA1841 is targeted to the

Interacting proteins

KIAA1841 was found to interact with

SRPK1 Serine/arginine-Rich Splicing Factor (SRSF) protein kinase-1 SRPK1 is an enzyme that in humans is encoded by the ''SRPK1'' gene. Function This gene encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) fami ...

(Serine/arginine- rich protein-specific kinase 1) The interaction was detected via a protein kinase assay. SRPK1 localizes to the nucleus and the cytoplasm. By regulating intracellular localization of splicing factors it is thought to play a role in regulating both constitutive and alternative splicing. KIAA1841 is also found in the nucleus and is thought to play a role in regulating

Clinical significance

Disease association

Diseases associated with this gene are

Crohn’s disease Crohn's disease is a type of inflammatory bowel disease (IBD) that may affect any segment of the gastrointestinal tract. Symptoms often include abdominal pain, diarrhea (which may be bloody if inflammation is severe), fever, abdominal distension, ...

celiac disease Coeliac disease (British English) or celiac disease (American English) is a long-term autoimmune disorder, primarily affecting the small intestine, where individuals develop intolerance to gluten, present in foods such as wheat, rye and barl ...

and

inflammatory bowel disease Inflammatory bowel disease (IBD) is a group of inflammation, inflammatory conditions of the colon (anatomy), colon and small intestine, Crohn's disease and ulcerative colitis being the principal types. Crohn's disease affects the small intestine a ...

.{{cite web, title=Genecards, url=https://www.genecards.org/cgi-bin/carddisp.pl?gene=KIAA1841&search=KIAA1841, publisher=The Gene Human Database