''Hanseniaspora pseudoguilliermondii'' is a species of

yeast Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom. The first yeast originated hundreds of millions of years ago, and at least 1,500 species are currently recognized. They are estimated to constitut ...

in the family

Saccharomycetaceae The Saccharomycetaceae are a family of yeasts in the order Saccharomycetales that reproduce by budding. Species in the family have a cosmopolitan distribution, and are present in a wide variety of habitats, especially those with a plentiful suppl ...

. Originally isolated from orange juice concentrate, it has been found on fruit and fruit juices in locations around the world. It has also been observed forming hybrids with '' Hanseniaspora opuntiae''.

Taxonomy

A sample of ''H. pseudoguilliermondii'' was first isolated from orange juice concentrate in

Georgia Georgia most commonly refers to: * Georgia (country), a country in the Caucasus region of Eurasia * Georgia (U.S. state), a state in the Southeast United States Georgia may also refer to: Places Historical states and entities * Related to the ...

, USA. It was studied in 2003 by Neža Čadež, Gé A. Poot, Peter Raspor, and Maudy Th. Smith, who found that it could not be distinguished from ''

Hanseniaspora guilliermondii ''Hanseniaspora guilliermondii'' is a species of yeast in the family Saccharomycetaceae. In its anamorph form, it is called ''Kloeckera apis''. Taxonomy The initial sample of the species was isolated by South African pathologist Adrianus Pij ...

'' using physiological criteria. After further testing in 2006, Čadež, Raspor, and Smith offered a description of the species, based upon DNA testing, that they called ''Hanseniaspora pseudoguilliermondii''. The

specific epithet In taxonomy, binomial nomenclature ("two-term naming system"), also called nomenclature ("two-name naming system") or binary nomenclature, is a formal system of naming species of living things by giving each a name composed of two parts, bot ...

"pseudoguilliermondii" was chosen because the species is similar to ''H. guilliermondii''.

Description

Microscopic examination of the yeast cells in YM liquid medium after 48 hours at 25°C reveals cells that are 2.2 to 8.7 μm by 1.6 to 4.2 μm in size,

apiculate The following is a list of terms which are used to describe leaf morphology in the description and taxonomy of plants. Leaves may be simple (a single leaf blade or lamina) or compound (with several leaflets). The edge of the leaf may be regular o ...

ovoid An oval () is a closed curve in a plane which resembles the outline of an egg. The term is not very specific, but in some areas (projective geometry, technical drawing, etc.) it is given a more precise definition, which may include either one or ...

to elongate, appearing singly or in pairs. Reproduction is by

budding Budding or blastogenesis is a type of asexual reproduction in which a new organism develops from an outgrowth or bud due to cell division at one particular site. For example, the small bulb-like projection coming out from the yeast cell is know ...

, which occurs at both poles of the cell. In broth culture, sediment is present, and after one month a very thin ring and a sediment is formed. Colonies that are grown on malt agar for one month at 25°C appear cream-colored, butyrous, glossy, and smooth. Growth is flat to slightly raised at the center, with an entire to slightly undulating margin. The yeast forms poorly developed

pseudohyphae A hypha (; ) is a long, branching, filamentous structure of a fungus, oomycete, or actinobacterium. In most fungi, hyphae are the main mode of vegetative growth, and are collectively called a mycelium. Structure A hypha consists of one or ...

on cornmeal or potato agar. The yeast has been observed to form four hat-shaped

ascospores An ascus (; ) is the sexual spore-bearing cell produced in ascomycete fungi. Each ascus usually contains eight ascospores (or octad), produced by meiosis followed, in most species, by a mitotic cell division. However, asci in some genera or ...

when grown for at least seven days on 5% Difco malt extract agar. The yeast can ferment

glucose Glucose is a simple sugar with the molecular formula . Glucose is overall the most abundant monosaccharide, a subcategory of carbohydrates. Glucose is mainly made by plants and most algae during photosynthesis from water and carbon dioxide, using ...

and

cellobiose Cellobiose is a disaccharide with the formula (C6H7(OH)4O)2O. It is classified as a reducing sugar. In terms of its chemical structure, it is derived from the condensation of a pair of β-glucose molecules forming a β(1→4) bond. It can be hyd ...

, but not

galactose Galactose (, '' galacto-'' + '' -ose'', "milk sugar"), sometimes abbreviated Gal, is a monosaccharide sugar that is about as sweet as glucose, and about 65% as sweet as sucrose. It is an aldohexose and a C-4 epimer of glucose. A galactose molec ...

sucrose Sucrose, a disaccharide, is a sugar composed of glucose and fructose subunits. It is produced naturally in plants and is the main constituent of white sugar. It has the molecular formula . For human consumption, sucrose is extracted and refined ...

maltose } Maltose ( or ), also known as maltobiose or malt sugar, is a disaccharide formed from two units of glucose joined with an α(1→4) bond. In the isomer isomaltose, the two glucose molecules are joined with an α(1→6) bond. Maltose is the two- ...

lactose Lactose is a disaccharide sugar synthesized by galactose and glucose subunits and has the molecular formula C12H22O11. Lactose makes up around 2–8% of milk (by mass). The name comes from ' (gen. '), the Latin word for milk, plus the suffix '' - ...

raffinose Raffinose is a trisaccharide composed of galactose, glucose, and fructose. It can be found in beans, cabbage, brussels sprouts, broccoli, asparagus, other vegetables, and whole grains. Raffinose can be hydrolyzed to D-galactose and sucrose by ...

trehalose Trehalose (from Turkish '' tıgala'' – a sugar derived from insect cocoons + -ose) is a sugar consisting of two molecules of glucose. It is also known as mycose or tremalose. Some bacteria, fungi, plants and invertebrate animals synthesize it ...

. It has a positive growth rate at 37°C, but there is no growth at 40°C. It can grow on agar media containing 0.1% cycloheximide and 10% sodium but growth on 50% glucose-yeast extract agar is weak.

Ecology

The original strain of this species was isolated from orange juice concentrate. It has also been isolated from fruit and fermenting fruit juices in The

Philippines The Philippines (; fil, Pilipinas, links=no), officially the Republic of the Philippines ( fil, Republika ng Pilipinas, links=no), * bik, Republika kan Filipinas * ceb, Republika sa Pilipinas * cbk, República de Filipinas * hil, Republ ...

Réunion Réunion (; french: La Réunion, ; previously ''Île Bourbon''; rcf, label= Reunionese Creole, La Rényon) is an island in the Indian Ocean that is an overseas department and region of France. It is located approximately east of the island ...

, and

French Guiana French Guiana ( or ; french: link=no, Guyane ; gcr, label=French Guianese Creole, Lagwiyann ) is an overseas departments and regions of France, overseas department/region and single territorial collectivity of France on the northern Atlantic ...

. It has been observed to form hybrids with '' Hanseniaspora opuntiae''. It is not known whether it has any human pathogenic potential, but it can grow at a normal body temperature.

References

{{Taxonbar, from=Q10517391 Saccharomycetes Yeasts Fungi described in 2006