''Halanaerobium hydrogeniformans'' is an

alkaliphilic Alkaliphiles are a class of extremophilic microbes capable of survival in alkaline ( pH roughly 8.5–11) environments, growing optimally around a pH of 10. These bacteria can be further categorized as obligate alkaliphiles (those that require high ...

bacterium that is capable of biohydrogen production at ( pH 11 and 7% (w/v)

sodium chloride Sodium chloride , commonly known as salt (although sea salt also contains other chemical salts), is an ionic compound with the chemical formula NaCl, representing a 1:1 ratio of sodium and chloride ions. With molar masses of 22.99 and 35.45 g ...

(NaCl) salts) and ; it is commonly found in haloalkaline lakes. ''H. hydrogeniformans'' was first discovered by Melanie Mormile, professor of biological sciences at Missouri University of Science and Technology and her team, from

Soap Lake Soap Lake is a meromictic soda lake in the town of Soap Lake, Washington formed by the Missoula Floods at the foot of the Grand Coulee. The lake gets its name from the naturally occurring foam that gives its water a soapy appearance, and becaus ...

Washington Washington commonly refers to: * Washington (state), United States * Washington, D.C., the capital of the United States ** A metonym for the federal government of the United States ** Washington metropolitan area, the metropolitan area centered on ...

Microbiology

''Halanaerobium hydrogeniformans'' is an obligately anaerobic,

Gram-negative Gram-negative bacteria are bacteria that do not retain the crystal violet stain used in the Gram staining method of bacterial differentiation. They are characterized by their cell envelopes, which are composed of a thin peptidoglycan cell wall ...

, non-motile, non-sporulating, elongated rod bacterium, which can tolerate the extreme conditions of both high

salinity Salinity () is the saltiness or amount of salt dissolved in a body of water, called saline water (see also soil salinity). It is usually measured in g/L or g/kg (grams of salt per liter/kilogram of water; the latter is dimensionless and equal ...

and alkalinity of its environment, and which has optimal growth at pH 11 and 7% NaCl. It can ferment a variety of 5- and 6-

carbon Carbon () is a chemical element with the symbol C and atomic number 6. It is nonmetallic and tetravalent In chemistry, the valence (US spelling) or valency (British spelling) of an element is the measure of its combining capacity with o ...

sugars derived from

cellulose Cellulose is an organic compound with the formula , a polysaccharide consisting of a linear chain of several hundred to many thousands of β(1→4) linked D-glucose units. Cellulose is an important structural component of the primary cell wall ...

and hemicelluloses, including

cellobiose Cellobiose is a disaccharide with the formula (C6H7(OH)4O)2O. It is classified as a reducing sugar. In terms of its chemical structure, it is derived from the condensation of a pair of β-glucose molecules forming a β(1→4) bond. It can be hyd ...

, and forms the end products

hydrogen Hydrogen is the chemical element with the symbol H and atomic number 1. Hydrogen is the lightest element. At standard conditions hydrogen is a gas of diatomic molecules having the formula . It is colorless, odorless, tasteless, non-toxic, an ...

acetate An acetate is a salt (chemistry), salt formed by the combination of acetic acid with a base (e.g. Alkali metal, alkaline, Alkaline earth metal, earthy, Transition metal, metallic, nonmetallic or radical Radical (chemistry), base). "Acetate" als ...

, and

formate Formate (IUPAC name: methanoate) is the conjugate base of formic acid. Formate is an anion () or its derivatives such as ester of formic acid. The salts and esters are generally colorless.Werner Reutemann and Heinz Kieczka "Formic Acid" in ''Ull ...

Genome structure

The ''H. hydrogeniformans''

genome In the fields of molecular biology and genetics, a genome is all the genetic information of an organism. It consists of nucleotide sequences of DNA (or RNA in RNA viruses). The nuclear genome includes protein-coding genes and non-coding ge ...

sequence was determined through a combination of Illumina and

454 Year 454 ( CDLIV) was a common year starting on Friday (link will display the full calendar) of the Julian calendar. At the time, it was known as the Year of the Consulship of Aetius and Studius (or, less frequently, year 1207 '' Ab urbe condi ...

technologies. The total genome size is 2,613,116 bp. The genome is 33.1% G+C and contains 2,295

candidate A candidate, or nominee, is the prospective recipient of an award or honor, or a person seeking or being considered for some kind of position; for example: * to be elected to an office — in this case a candidate selection procedure occurs. * t ...

protein Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, respo ...

-encoding

gene In biology, the word gene (from , ; "...Wilhelm Johannsen coined the word gene to describe the Mendelian units of heredity..." meaning ''generation'' or ''birth'' or ''gender'') can have several different meanings. The Mendelian gene is a ba ...

models. The

contains four separate

ribosomal RNA Ribosomal ribonucleic acid (rRNA) is a type of non-coding RNA which is the primary component of ribosomes, essential to all cells. rRNA is a ribozyme which carries out protein synthesis in ribosomes. Ribosomal RNA is transcribed from ribosomal ...

(rRNA) operons, each containing a 5S, a 16S, and a 23S rRNA gene, with 99.9 to 100% identity between 16S rRNA genes.

Applications

As the price of

fossil fuel A fossil fuel is a hydrocarbon-containing material formed naturally in the Earth's crust from the remains of dead plants and animals that is extracted and burned as a fuel. The main fossil fuels are coal, oil, and natural gas. Fossil fuels m ...

s increases and reserves diminish,

biofuel Biofuel is a fuel that is produced over a short time span from biomass, rather than by the very slow natural processes involved in the formation of fossil fuels, such as oil. According to the United States Energy Information Administration (E ...

production is seen as a viable contribution to current and future energy demands. ''Halanaerobium hydrogeniformans'' can use several pure sugars for

production. Hence, this bacterium can potentially increase the efficiency and efficacy of biohydrogen production from renewable

biomass Biomass is plant-based material used as a fuel for heat or electricity production. It can be in the form of wood, wood residues, energy crops, agricultural residues, and waste from industry, farms, and households. Some people use the terms bi ...

resources.

References

{{Taxonbar, from=Q20687153 Halanaerobiales Bacteria described in 2011

Microbiology

Genome structure

Applications

See also

References