Cytochrome b within both
molecular and
cell biology, is a protein found in the
mitochondria of
eukaryotic
Eukaryotes () are organisms whose Cell (biology), cells have a cell nucleus, nucleus. All animals, plants, fungi, and many unicellular organisms, are Eukaryotes. They belong to the group of organisms Eukaryota or Eukarya, which is one of the ...
cells. It functions as part of the
electron transport chain and is the main subunit of transmembrane
cytochrome bc1
Cytochromes are redox-active proteins containing a heme, with a central Fe atom at its core, as a cofactor. They are involved in electron transport chain and redox catalysis. They are classified according to the type of heme and its mode of bi ...
and
b6f complexes.
Function
In the mitochondrion of
eukaryotes and in aerobic
prokaryotes, cytochrome b is a component of
respiratory chain
An electron transport chain (ETC) is a series of protein complexes and other molecules that transfer electrons from electron donors to electron acceptors via redox reactions (both reduction and oxidation occurring simultaneously) and couples thi ...
complex III () — also known as the bc1 complex or ubiquinol-cytochrome c reductase. In plant
chloroplasts
A chloroplast () is a type of membrane-bound organelle known as a plastid that conducts photosynthesis mostly in plant and algal cells. The photosynthetic pigment chlorophyll captures the energy from sunlight, converts it, and stores it in ...
and
cyanobacteria, there is an analogous protein, cytochrome b6, a component of the plastoquinone-plastocyanin reductase (), also known as the b6f complex. These complexes are involved in electron transport, the pumping of protons to create a proton-motive force (
PMF). This
proton gradient
An electrochemical gradient is a gradient of electrochemical potential, usually for an ion that can move across a membrane. The gradient consists of two parts, the chemical gradient, or difference in solute concentration across a membrane, and th ...
is used for the generation of
ATP. These complexes play a vital role in cells.
Structure
Cytochrome b/b6
is an integral
membrane protein
Membrane proteins are common proteins that are part of, or interact with, biological membranes. Membrane proteins fall into several broad categories depending on their location. Integral membrane proteins are a permanent part of a cell membrane ...
of approximately 400
amino acid residues that probably has 8
transmembrane segment
A transmembrane domain (TMD) is a membrane-spanning protein domain. TMDs generally adopt an alpha helix topological conformation, although some TMDs such as those in porins can adopt a different conformation. Because the interior of the lipid bi ...
s. In plants and cyanobacteria, cytochrome b6 consists of two
protein subunits encoded by the petB and petD genes. Cytochrome b/b6 non-covalently binds two
heme
Heme, or haem (pronounced / hi:m/ ), is a precursor to hemoglobin, which is necessary to bind oxygen in the bloodstream. Heme is biosynthesized in both the bone marrow and the liver.
In biochemical terms, heme is a coordination complex "consis ...
groups, known as b562 and b566. Four conserved histidine residues are postulated to be the
ligands of the iron atoms of these two heme groups.
Use in phylogenetics
Cytochrome b is commonly used as a region of
mitochondrial DNA for determining
phylogenetic
In biology, phylogenetics (; from Greek φυλή/ φῦλον [] "tribe, clan, race", and wikt:γενετικός, γενετικός [] "origin, source, birth") is the study of the evolutionary history and relationships among or within groups o ...
relationships between organisms, due to its sequence variability. It is considered to be most useful in determining relationships within
families
Family (from la, familia) is a group of people related either by consanguinity (by recognized birth) or affinity (by marriage or other relationship). The purpose of the family is to maintain the well-being of its members and of society. Ideal ...
and
genera. Comparative studies involving cytochrome b have resulted in new classification schemes and have been used to assign newly described species to a genus as well as to deepen the understanding of evolutionary relationships.
Clinical significance
Mutations in cytochrome b primarily result in
exercise intolerance
Exercise intolerance is a condition of inability or decreased ability to perform physical exercise at the normally expected level or duration for people of that age, size, sex, and muscle mass. It also includes experiences of unusually severe pos ...
in human patients; though more rare, severe multi-system pathologies have also been reported.
Single-point mutations in cytochrome b of ''Plasmodium falciparum'' and ''P. berghei'' are associated with resistance to the anti-malarial drug
atovaquone.
Human genes
Human genes encoding cytochrome b proteins include:
*
CYB5A
Cytochrome b5, form A (gene name CYB5A), is a human microsomal cytochrome b5.
Cytochrome b5 is a membrane bound hemoprotein which functions as an electron carrier for several membrane bound oxygenases. It has two isoforms produced by alternative ...
– cytochrome b5 type A (microsomal)
*
CYB5B
Cytochrome b is a protein that in humans is encoded by the ''MT-CYB'' gene. Its gene product is a subunit of the respiratory chain protein ubiquinol–cytochrome ''c'' reductase (UQCR, complex III or cytochrome ''bc''1 complex), which consists ...
– cytochrome b5 type B (outer mitochondrial membrane)
*
CYBASC3 – cytochrome b, ascorbate dependent 3
*
MT-CYB
Cytochrome b is a protein that in humans is encoded by the ''MT-CYB'' gene. Its gene product is a subunit of the respiratory chain protein ubiquinol–cytochrome ''c'' reductase (UQCR, complex III or cytochrome ''bc''1 complex), which consists ...
– mitochondrially encoded cytochrome b
Fungicide target
Cyt b is targeted by the
QI class of fungicides,
Fungicide Resistance Action Committee
Fungicides are biocidal chemical compounds or biological organisms used to kill parasitic fungi or their spores. A fungistatic inhibits their growth. Fungi can cause serious damage in agriculture, resulting in critical losses of yield, quality, ...
group 11. The cyt b mutations and provide resistance against the main body of group 11, although G143A does not work against
metyltetraprole (11A).
G143A is significant
in ''Botrytis cinerea'' in California strawberry production.
[
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References
External links
*
{{Hemeproteins
Protein domains
Protein families
Transmembrane proteins