![Agarplates](https://upload.wikimedia.org/wikipedia/commons/7/7c/Agarplates.jpg)
Bismuth sulfite agar is a type of
agar media used to isolate ''
Salmonella'' species. It uses
glucose
Glucose is a simple sugar with the molecular formula . Glucose is overall the most abundant monosaccharide, a subcategory of carbohydrates. Glucose is mainly made by plants and most algae during photosynthesis from water and carbon dioxide, u ...
as a primary source of
carbon
Carbon () is a chemical element with the symbol C and atomic number 6. It is nonmetallic and tetravalent—its atom making four electrons available to form covalent chemical bonds. It belongs to group 14 of the periodic table. Carbon mak ...
.
BLBG and bismuth stop
gram-positive
In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall.
Gram-positive bact ...
growth.
Bismuth sulfite agar tests the ability to use
ferrous sulfate
Iron(II) sulfate (British English: iron(II) sulphate) or ferrous sulfate denotes a range of salts with the formula Fe SO4·''x''H2O. These compounds exist most commonly as the hepta hydrate (''x'' = 7) but several values for x are kn ...
and convert it to
hydrogen sulfide.
Bismuth sulfite agar typically contains (
w/v):
::1.6%
bismuth sulfite Bi
2(SO
3)
3
::1.0% pancreatic
digest of
casein
Casein ( , from Latin ''caseus'' "cheese") is a family of related phosphoproteins ( αS1, aS2, β, κ) that are commonly found in mammalian milk, comprising about 80% of the proteins in cow's milk and between 20% and 60% of the proteins in hum ...
::1.0% pancreatic digest of animal
tissue
::1.0%
beef extract
::1.0%
glucose
Glucose is a simple sugar with the molecular formula . Glucose is overall the most abundant monosaccharide, a subcategory of carbohydrates. Glucose is mainly made by plants and most algae during photosynthesis from water and carbon dioxide, u ...
::0.8% dibasic
sodium phosphate
Sodium phosphate is a generic term for a variety of salts of sodium (Na+) and phosphate (PO43−). Phosphate also forms families or condensed anions including di-, tri-, tetra-, and polyphosphates. Most of these salts are known in both anhyd ...
::0.06%
ferrous sulfate
Iron(II) sulfate (British English: iron(II) sulphate) or ferrous sulfate denotes a range of salts with the formula Fe SO4·''x''H2O. These compounds exist most commonly as the hepta hydrate (''x'' = 7) but several values for x are kn ...
• 7 water
::pH adjusted to 7.7 at 25 °C
This medium is boiled for sterility, not
autoclave
An autoclave is a machine used to carry out industrial and scientific processes requiring elevated temperature and pressure in relation to ambient pressure and/or temperature. Autoclaves are used before surgical procedures to perform sterilizati ...
d.
References
Microbiological media
Bismuth
{{Microbiology-stub