Stages
Amelogenesis is considered to have three stages. The first stage is known as the inductive stage, the second is the secretory stage, and the third stage is known as the maturation stage. During the inductive stage, ameloblast differentiation from IEE is initiated. Proteins and an organic matrix form a partially mineralized enamel in the secretory stage. The maturation stage completes enamel mineralization.Inductive (or pre-secretory) stage
In the inductive stage, the morphodifferentiation phase the shape of the crown is determined by the bell stage of tooth development. There is a basal lamina between the IEE and the dental papilla. At this time, the dentin is not mineralized. The IEE cuboidal or low columnar with centralized nuclei and poorly developed Golgi complexes. The differentiation phase of the Induction stage is initiated by the presence of newly formed predentin. The IEE cells then elongate and become preameloblasts. There is a shift in polarity. Each preameloblast elongates and becomes an postmitotic, polarized, secretory ameloblast. However, there are no Tomes' process yet. It is at this stage that a signal is sent from the newly differentiated ameloblasts back across the dentinoenamel junction (DEJ) to stimulate dentinogenesis.Secretory stage
In the secretory stage, ameloblasts are polarized columnarMaturation stage
In the maturation stage, the ameloblasts transport substances used in the formation of enamel. Microscopically, the most notable aspect of this phase is that these cells become striated, or have a ruffled border. These signs demonstrate that the ameloblasts have changed their function from production, as in the secretory stage, to transportation. Proteins used for the mineralization process compose most of the material transported into the matrix, importantly amelogenins, ameloblastins, enamelins, and tuftelins. The Ca2+ mainly comes from the enamel organ, and not the dental papilla, by either passive, extracellular transportation or active, intracellular transportation. The active route is controlled by ameloblasts so the site of mineralization can have a tightly controlled climate, including modulation of proteins that inhibit mineralization (e.g. Serum-derived Albumin) and concentration of ions. As enamel is secreted, some mineralisation occurs by Ca2+ deposition between nanospheres of amelogenins forming crystallites. Tuftelin also is suggested to have a role in directing the initial deposition. The undermineralised, immature enamel containing long, thin prisms of hydroxyapatite, now matures. As the prisms in the enamel grow in thickness but not length, proteins (amelogenins and most non-amelogenins) are removed from the matrix to give more space for hydroxyapatite deposition - mature crystals are hexagonal and 25x75nm and can run the whole length of the enamel (up to 2.5mm). The mineralising enamel becomes progressively less porous. During this process, enamelins and tuftelin are left in the enamel (responsible for enamel tufts). By the end of this stage, the enamel has completed its mineralization. Enamel mineralization only occurs once (as ameloblasts are lost with eruption within the reduced enamel epithelium); therefore after amelogenesis, enamel production has been finalized.Max A. Listgarten, University of Pennsylvania and Temple University, http://www.dental.pitt.edu/informatics/periohistology/en/gu0302.htm This is in contrast toSee also
* Ameloblast * Amelogenesis imperfecta * Tooth development * Tooth enamelReferences