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Scintillation Proximity Assay
Scintillation proximity assay (SPA) is an assay development and biochemical screening that permits the rapid and sensitive measurement of a broad range of biological processes in a homogeneous system. The type of beads that are involved in the SPA are microscopic in size and within the beads itself, there is a scintillant which emits light when it is stimulated. Stimulation occurs when radio-labelled molecules interact and bind to the surface of the bead. This interaction will trigger the bead to emit light, which can be detected using a photometer. Overview The SPA technique is dependent on the energy conversion of radioactive decay, which releases light photons which can be detected via the use of some devices such as the photomultiplier tubes of scintillation counters or CCD imagers. This is a very popular technique in practices that require detecting and quantifying radioactivity.Homogeneous Proximity Tyrosine Kinase Assays: Scintillation Proximity Assay versus Homogeneous Tim ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Scintillation (physics)
Scintillation is the physical process where a material, called scintillator, emits UV or visible light under excitation from high energy photons (X-rays or γ-rays) or energetic particles,(such as electrons, alpha particles, neutrons or ions). See scintillator and scintillation counter for practical applications. Overview The process of scintillation is one of luminescence whereby light of a characteristic spectrum is emitted following the absorption of radiation. The scintillation process can be summarized in three main stages (A) conversion, (B) transport and energy transfer to the luminescence center, and (C) luminescence. The emitted radiation is usually less energetic than the absorbed radiation, hence generally scintillation is a down-conversion process. Conversion processes The first stage of scintillation, conversion, is the process where the energy from the incident radiation is absorbed by the scintillator and highly energetic electrons and holes are created in th ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Subatomic Particle
In physical sciences, a subatomic particle is a particle that composes an atom. According to the Standard Model of particle physics, a subatomic particle can be either a composite particle, which is composed of other particles (for example, a proton, neutron, or meson), or an elementary particle, which is not composed of other particles (for example, an electron, photon, or muon). Particle physics and nuclear physics study these particles and how they interact. Experiments show that light could behave like a stream of particles (called photons) as well as exhibiting wave-like properties. This led to the concept of wave–particle duality to reflect that quantum-scale behave like both particles and waves; they are sometimes called wavicles to reflect this. Another concept, the uncertainty principle, states that some of their properties taken together, such as their simultaneous position and momentum, cannot be measured exactly. The wave–particle duality has been shown to app ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Assay
An assay is an investigative (analytic) procedure in laboratory medicine, mining, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a target entity. The measured entity is often called the analyte, the measurand, or the target of the assay. The analyte can be a drug, biochemical substance, chemical element or compound, or cell in an organism or organic sample. An assay usually aims to measure an analyte's intensive property and express it in the relevant measurement unit (e.g. molarity, density, functional activity in enzyme international units, degree of effect in comparison to a standard, etc.). If the assay involves exogenous reactants (the reagents), then their quantities are kept fixed (or in excess) so that the quantity and quality of the target are the only limiting factors. The difference in the assay outcome is used to deduce the unknown quality or quantity o ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Enzyme
Enzymes () are proteins that act as biological catalysts by accelerating chemical reactions. The molecules upon which enzymes may act are called substrates, and the enzyme converts the substrates into different molecules known as products. Almost all metabolic processes in the cell need enzyme catalysis in order to occur at rates fast enough to sustain life. Metabolic pathways depend upon enzymes to catalyze individual steps. The study of enzymes is called ''enzymology'' and the field of pseudoenzyme analysis recognizes that during evolution, some enzymes have lost the ability to carry out biological catalysis, which is often reflected in their amino acid sequences and unusual 'pseudocatalytic' properties. Enzymes are known to catalyze more than 5,000 biochemical reaction types. Other biocatalysts are catalytic RNA molecules, called ribozymes. Enzymes' specificity comes from their unique three-dimensional structures. Like all catalysts, enzymes increase the reaction ra ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Telecentric Lens
A telecentric lens is a special optical lens (often an objective lens or a camera lens) that has its entrance or exit pupil, or both, at infinity. Telecentric lenses are often used for precision optical two-dimensional measurements or reproduction and other applications that are sensitive to the image magnification or the angle of incidence of light. The simplest way to make a lens telecentric is to put the aperture stop at one of the lens's focal points. This makes the chief rays (light rays that pass through the center of the aperture) on the other side of the lens parallel to the optical axis for any point in the field of view. Commercially available telecentric lenses are often ''compound lenses'' that include multiple lens elements, for improved optical performance. Telecentricity is not a property of the lenses inside the compound lens but is established by the location of the aperture stop in the lens. The aperture stop selects the rays that are passed through the lens an ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Digital Camera
A digital camera is a camera that captures photographs in digital memory. Most cameras produced today are digital, largely replacing those that capture images on photographic film. Digital cameras are now widely incorporated into mobile devices like smartphones with the same or more capabilities and features of dedicated cameras (which are still available). High-end, high-definition dedicated cameras are still commonly used by professionals and those who desire to take higher-quality photographs. Digital and digital movie cameras share an optical system, typically using a lens with a variable diaphragm to focus light onto an image pickup device. The diaphragm and shutter admit a controlled amount of light to the image, just as with film, but the image pickup device is electronic rather than chemical. However, unlike film cameras, digital cameras can display images on a screen immediately after being recorded, and store and delete images from memory. Many digital cameras can ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Electrode
An electrode is an electrical conductor used to make contact with a nonmetallic part of a circuit (e.g. a semiconductor, an electrolyte, a vacuum or air). Electrodes are essential parts of batteries that can consist of a variety of materials depending on the type of battery. The electrophore, invented by Johan Wilcke, was an early version of an electrode used to study static electricity. Anode and cathode in electrochemical cells Electrodes are an essential part of any battery. The first electrochemical battery made was devised by Alessandro Volta and was aptly named the Voltaic cell. This battery consisted of a stack of copper and zinc electrodes separated by brine-soaked paper disks. Due to fluctuation in the voltage provided by the voltaic cell it wasn't very practical. The first practical battery was invented in 1839 and named the Daniell cell after John Frederic Daniell. Still making use of the zinc–copper electrode combination. Since then many more batteries have be ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Photocathode
A photocathode is a surface engineered to convert light (photons) into electrons using the photoelectric effect. Photocathodes are important in accelerator physics where they are utilised in a photoinjector to generate high brightness electron beams. Electron beams generated with photocathodes are commonly used for free electron lasers and for ultrafast electron diffraction. Photocathodes are also commonly used as the negatively charged electrode in a light detection device such as a photomultiplier or phototube. Important Properties Quantum Efficiency (QE) Quantum efficiency is a unitless number that measures the sensitivity of the photocathode to light. It is the ratio of the number of electrons emitted to the number of incident photons.Rao, T., & Dowell, D. H. (2013). ''An engineering guide to photoinjectors''. CreateSpace Independent Publishing. This property depends on the wavelength of light being used to illuminate the photocathode. For many applications, QE is th ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Electrical Energy
Electrical energy is energy related to forces on electrically charged particles and the movement of electrically charged particles (often electrons in wires, but not always). This energy is supplied by the combination of electric current and electric potential (often referred to as voltage because electric potential is measured in volts) that is delivered by an electrical circuit (e.g., provided by an electric power utility). Motion (current) is not required; for example, if there is a voltage difference in combination with charged particles, such as static electricity or a charged capacitor, the moving electrical energy is typically converted to another form of energy (e.g., thermal, motion, sound, light, radio waves, etc.). Electrical energy is usually sold by the kilowatt hour (1 kW·h = 3.6 MJ) which is the product of the power in kilowatts multiplied by running time in hours. Electric utilities measure energy using an electricity meter, which keeps a running total of the elec ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Radiation Length
In physics, the radiation length is a characteristic of a material, related to the energy loss of high energy particles electromagnetically interacting with it. Definition In materials of high atomic number (e.g. W, U, Pu) the electrons of energies >~10 MeV predominantly lose energy by bremsstrahlung, and high-energy photons by pair production. The characteristic amount of matter traversed for these related interactions is called the radiation length , usually measured in g·cm−2. It is both the mean distance over which a high-energy electron loses all but of its energy by bremsstrahlung, and of the mean free path for pair production by a high-energy photon. It is also the appropriate length scale for describing high-energy electromagnetic cascades. The radiation length for a given material consisting of a single type of nucleus can be approximated by the following expression: (http://pdg.lbl.gov/) X_0 = 716.4\;\mathrm g\, \mathrm^ \frac = 1433 \;\mathrm g\, \mathrm^ \fra ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Tritium
Tritium ( or , ) or hydrogen-3 (symbol T or H) is a rare and radioactive isotope of hydrogen with half-life about 12 years. The nucleus of tritium (t, sometimes called a ''triton'') contains one proton and two neutrons, whereas the nucleus of the common isotope hydrogen-1 (''protium'') contains one proton and zero neutrons, and that of hydrogen-2 (''deuterium'') contains one proton and one neutron. Naturally occurring tritium is extremely rare on Earth. The atmosphere has only trace amounts, formed by the interaction of its gases with cosmic rays. It can be produced artificially by irradiating lithium metal or lithium-bearing ceramic pebbles in a nuclear reactor and is a low-abundance byproduct in normal operations of nuclear reactors. Tritium is used as the energy source in radioluminescent lights for watches, gun sights, numerous instruments and tools, and even novelty items such as self-illuminating key chains. It is used in a medical and scientific setting as a radioacti ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Electron
The electron ( or ) is a subatomic particle with a negative one elementary electric charge. Electrons belong to the first generation of the lepton particle family, and are generally thought to be elementary particles because they have no known components or substructure. The electron's mass is approximately 1/1836 that of the proton. Quantum mechanical properties of the electron include an intrinsic angular momentum ( spin) of a half-integer value, expressed in units of the reduced Planck constant, . Being fermions, no two electrons can occupy the same quantum state, in accordance with the Pauli exclusion principle. Like all elementary particles, electrons exhibit properties of both particles and waves: They can collide with other particles and can be diffracted like light. The wave properties of electrons are easier to observe with experiments than those of other particles like neutrons and protons because electrons have a lower mass and hence a longer de Broglie wavele ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
