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Phase Contrast
Phase-contrast imaging is a method of imaging that has a range of different applications. It exploits differences in the refractive index of different materials to differentiate between structures under analysis. In conventional light microscopy, phase contrast can be employed to distinguish between structures of similar transparency, and to examine crystals on the basis of their double refraction. This has uses in biological, medical and geological science. In X-ray tomography, the same physical principles can be used to increase image contrast by highlighting small details of differing refractive index within structures that are otherwise uniform. In transmission electron microscopy (TEM), phase contrast enables very high resolution (HR) imaging, making it possible to distinguish features a few Angstrom apart (at this point highest resolution is 40 pm). Light microscopy Phase contrast takes advantage of the fact that different structures have different refractive indices, and e ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Image
An image is a visual representation of something. It can be two-dimensional, three-dimensional, or somehow otherwise feed into the visual system to convey information. An image can be an artifact, such as a photograph or other two-dimensional picture, that resembles a subject. In the context of signal processing, an image is a distributed amplitude of color(s). In optics, the term “image” may refer specifically to a 2D image. An image does not have to use the entire visual system to be a visual representation. A popular example of this is of a greyscale image, which uses the visual system's sensitivity to brightness across all wavelengths, without taking into account different colors. A black and white visual representation of something is still an image, even though it does not make full use of the visual system's capabilities. Images are typically still, but in some cases can be moving or animated. Characteristics Images may be two or three- dimensional, such as a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
X-ray Optics
X-ray optics is the branch of optics that manipulates X-rays instead of visible light. It deals with focusing and other ways of manipulating the X-ray beams for research techniques such as X-ray crystallography, X-ray fluorescence, small-angle X-ray scattering, X-ray microscopy, X-ray phase-contrast imaging, and X-ray astronomy. Since X-rays and visible light are both electromagnetic waves they propagate in space in the same way, but because of the much higher frequency and photon energy of X-rays they interact with matter very differently. Visible light is easily redirected using lenses and mirrors, but because the real part of the complex refractive index of all materials is very close to 1 for X-rays, they instead tend to initially penetrate and eventually get absorbed in most materials without changing direction much. X-ray techniques There are many different techniques used to redirect X-rays, most of them changing the directions by only minute angles. The most common p ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Light Beam
A light beam or beam of light is a directional projection of light energy radiating from a light source. Sunlight forms a light beam (a sunbeam) when filtered through media such as clouds, foliage, or windows. To artificially produce a light beam, a lamp and a parabolic reflector is used in many lighting devices such as spotlights, car headlights, PAR Cans, and LED housings. Light from certain types of laser has the smallest possible beam divergence. Visible light beams From the side, a beam of light is only visible if part of the light is scattered by objects: tiny particles like dust, water droplets (mist, fog, rain), hail, snow, or smoke, or larger objects such as birds. If there are many objects in the light path, then it appears as a continuous beam, but if there are only a few objects, then the light is visible as a few individual bright points. In any case, this scattering of light from a beam, and the resultant visibility of a light beam from the side, is kn ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Deconvolution
In mathematics, deconvolution is the operation inverse to convolution. Both operations are used in signal processing and image processing. For example, it may be possible to recover the original signal after a filter (convolution) by using a deconvolution method with a certain degree of accuracy. Due to the measurement error of the recorded signal or image, it can be demonstrated that the worse the SNR, the worse the reversing of a filter will be; hence, inverting a filter is not always a good solution as the error amplifies. Deconvolution offers a solution to this problem. The foundations for deconvolution and time-series analysis were largely laid by Norbert Wiener of the Massachusetts Institute of Technology in his book ''Extrapolation, Interpolation, and Smoothing of Stationary Time Series'' (1949). The book was based on work Wiener had done during World War II but that had been classified at the time. Some of the early attempts to apply these theories were in the field ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Image Resolution
Image resolution is the detail an image holds. The term applies to digital images, film images, and other types of images. "Higher resolution" means more image detail. Image resolution can be measured in various ways. Resolution quantifies how close lines can be to each other and still be visibly ''resolved''. Resolution units can be tied to physical sizes (e.g. lines per mm, lines per inch), to the overall size of a picture (lines per picture height, also known simply as lines, TV lines, or TVL), or to angular subtense. Instead of single lines, line pairs are often used, composed of a dark line and an adjacent light line; for example, a resolution of 10 lines per millimeter means 5 dark lines alternating with 5 light lines, or 5 line pairs per millimeter (5 LP/mm). Photographic lens and film resolution are most often quoted in line pairs per millimeter. Types The resolution of digital cameras can be described in many different ways. Pixel count The term ''resolution'' is ofte ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Diffraction
Diffraction is defined as the interference or bending of waves around the corners of an obstacle or through an aperture into the region of geometrical shadow of the obstacle/aperture. The diffracting object or aperture effectively becomes a secondary source of the propagating wave. Italian scientist Francesco Maria Grimaldi coined the word ''diffraction'' and was the first to record accurate observations of the phenomenon in 1660. In classical physics, the diffraction phenomenon is described by the Huygens–Fresnel principle that treats each point in a propagating wavefront as a collection of individual spherical wavelets. The characteristic bending pattern is most pronounced when a wave from a coherent source (such as a laser) encounters a slit/aperture that is comparable in size to its wavelength, as shown in the inserted image. This is due to the addition, or interference, of different points on the wavefront (or, equivalently, each wavelet) that travel by paths of ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Transmission Electron Microscopy
Transmission electron microscopy (TEM) is a microscopy technique in which a beam of electrons is transmitted through a specimen to form an image. The specimen is most often an ultrathin section less than 100 nm thick or a suspension on a grid. An image is formed from the interaction of the electrons with the sample as the beam is transmitted through the specimen. The image is then magnified and focused onto an imaging device, such as a fluorescent screen, a layer of photographic film, or a sensor such as a scintillator attached to a charge-coupled device. Transmission electron microscopes are capable of imaging at a significantly higher resolution than light microscopes, owing to the smaller de Broglie wavelength of electrons. This enables the instrument to capture fine detail—even as small as a single column of atoms, which is thousands of times smaller than a resolvable object seen in a light microscope. Transmission electron microscopy is a major analytical method ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Embryo
An embryo is an initial stage of development of a multicellular organism. In organisms that reproduce sexually, embryonic development is the part of the life cycle that begins just after fertilization of the female egg cell by the male sperm cell. The resulting fusion of these two cells produces a single-celled zygote that undergoes many cell divisions that produce cells known as blastomeres. The blastomeres are arranged as a solid ball that when reaching a certain size, called a morula, takes in fluid to create a cavity called a blastocoel. The structure is then termed a blastula, or a blastocyst in mammals. The mammalian blastocyst hatches before implantating into the endometrial lining of the womb. Once implanted the embryo will continue its development through the next stages of gastrulation, neurulation, and organogenesis. Gastrulation is the formation of the three germ layers that will form all of the different parts of the body. Neurulation forms the nervou ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Metazoan
Animals are multicellular, eukaryotic organisms in the biological kingdom Animalia. With few exceptions, animals consume organic material, breathe oxygen, are able to move, can reproduce sexually, and go through an ontogenetic stage in which their body consists of a hollow sphere of cells, the blastula, during embryonic development. Over 1.5 million living animal species have been described—of which around 1 million are insects—but it has been estimated there are over 7 million animal species in total. Animals range in length from to . They have complex interactions with each other and their environments, forming intricate food webs. The scientific study of animals is known as zoology. Most living animal species are in Bilateria, a clade whose members have a bilaterally symmetric body plan. The Bilateria include the protostomes, containing animals such as nematodes, arthropods, flatworms, annelids and molluscs, and the deuterostomes, containing the echinoderms ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Precambrian
The Precambrian (or Pre-Cambrian, sometimes abbreviated pꞒ, or Cryptozoic) is the earliest part of Earth's history, set before the current Phanerozoic Eon. The Precambrian is so named because it preceded the Cambrian, the first period of the Phanerozoic Eon, which is named after Cambria, the Latinised name for Wales, where rocks from this age were first studied. The Precambrian accounts for 88% of the Earth's geologic time. The Precambrian is an informal unit of geologic time, subdivided into three eons ( Hadean, Archean, Proterozoic) of the geologic time scale. It spans from the formation of Earth about 4.6 billion years ago ( Ga) to the beginning of the Cambrian Period, about million years ago ( Ma), when hard-shelled creatures first appeared in abundance. Overview Relatively little is known about the Precambrian, despite it making up roughly seven-eighths of the Earth's history, and what is known has largely been discovered from the 1960s onwards. The Precambrian fos ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Density
Density (volumetric mass density or specific mass) is the substance's mass per unit of volume. The symbol most often used for density is ''ρ'' (the lower case Greek letter rho), although the Latin letter ''D'' can also be used. Mathematically, density is defined as mass divided by volume: : \rho = \frac where ''ρ'' is the density, ''m'' is the mass, and ''V'' is the volume. In some cases (for instance, in the United States oil and gas industry), density is loosely defined as its weight per unit volume, although this is scientifically inaccurate – this quantity is more specifically called specific weight. For a pure substance the density has the same numerical value as its mass concentration. Different materials usually have different densities, and density may be relevant to buoyancy, purity and packaging. Osmium and iridium are the densest known elements at standard conditions for temperature and pressure. To simplify comparisons of density across different syst ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Tomography
Tomography is imaging by sections or sectioning that uses any kind of penetrating wave. The method is used in radiology, archaeology, biology, atmospheric science, geophysics, oceanography, plasma physics, materials science, cosmochemistry, astrophysics, quantum information, and other areas of science. The word ''tomography'' is derived from Ancient Greek τόμος ''tomos'', "slice, section" and γράφω ''graphō'', "to write" or, in this context as well, "to describe." A device used in tomography is called a tomograph, while the image produced is a tomogram. In many cases, the production of these images is based on the mathematical procedure tomographic reconstruction, such as X-ray computed tomography technically being produced from multiple projectional radiographs. Many different reconstruction algorithms exist. Most algorithms fall into one of two categories: filtered back projection (FBP) and iterative reconstruction (IR). These procedures give inexact results: ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
