Bacterial Wilt Of Carnation
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Bacterial Wilt Of Carnation
Bacterial Wilt of Carnations is a bacterial disease caused by the plant pathogen ''Paraburkholderia caryophylli'' (often referred to as ''Burkholderia caryophylli''). Previously, named ''Pseudomonas caryophilli,'' the pathogen is an aerobic gram negative bacteria known for only being capable of entering its host through wounds. Once inside the host, it colonizes the vascular system and roots causing symptoms such as, internal stem cracking, yellowing of the leaves, wilting, and the development of cankers. As a bacterial disease, Bacterial Wilt of Carnations can also be characterized by signs such as bacterial streaming, and bacterial ooze. Hosts and symptoms ''B. caryophylli'' infects many different species of the Dianthus plant. The species ''Dianthus barbatus ''(Sweet William) and ''Dianthus alwoodii ''(Allwoodii Hybrids) can be infected through induced artificial inoculation in a lab setting, however, the pathogen only naturally infects the species ''D. caryophyllus'' (carnat ...
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Carnation
''Dianthus caryophyllus'' (), commonly known as the carnation or clove pink, is a species of ''Dianthus''. It is likely native to the Mediterranean region but its exact range is unknown due to extensive cultivation for the last 2,000 years.Med-Checklist''Dianthus caryophyllus''/ref>Flora Europaea''Dianthus caryophyllus''/ref>Blamey, M. & Grey-Wilson, C. (1989). ''Flora of Britain and Northern Europe''. Huxley, A., ed. (1992). ''New RHS Dictionary of Gardening''. Macmillan . Taxonomy Carnations were mentioned in Greek literature 2,000 years ago. The term ''dianthus'' was coined by Greek botanist Theophrastus, and is derived from the Ancient Greek words for divine ("dios") and flower ("anthos"). The name "carnation" is believed to come from the Latin ''corona-ae'', a "wreath, garland, chaplet, crown",Cassell's Latin Dictionary, Marchant, J.R.V, & Charles, Joseph F., (Eds.), Revised Edition, 1928 as it was one of the flowers used in Greek and Roman ceremonial crowns, or possibly ...
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Paraburkholderia Caryophylli
''Paraburkholderia'' is a genus of Pseudomonadota that are gram negative, slightly curved rods that are motile by means of flagella. They have been reported to colonize endophytic tissues of hybrid spruce (Picea glauca x engelmannii) and lodgepole pine with a strong potential to perform biological nitrogen fixation and plant growth promotion. Unlike ''Burkholderia'' species, ''Paraburkholderia'' members are not commonly associated with human infection. ''Paraburkholderia'' members form a monophyletic clade within the Burkholderiaceae family, which is what prompted their distinction as a genus independent from ''Burkholderia'' species, in combination with the finding of robust conserved signature indels which are unique to ''Paraburkholderia'' species, and are lacking in members of the genus ''Burkholderia''. These CSIs distinguish the genus from all other bacteria. Additionally, the CSIs that were found to be shared by ''Burkholderia'' species are absent in ''Paraburkholderia'' ...
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Vascular Tissue
Vascular tissue is a complex conducting tissue, formed of more than one cell type, found in vascular plants. The primary components of vascular tissue are the xylem and phloem. These two tissues transport fluid and nutrients internally. There are also two meristems associated with vascular tissue: the vascular cambium and the cork cambium. All the vascular tissues within a particular plant together constitute the vascular tissue system of that plant. The cells in vascular tissue are typically long and slender. Since the xylem and phloem function in the conduction of water, minerals, and nutrients throughout the plant, it is not surprising that their form should be similar to pipes. The individual cells of phloem are connected end-to-end, just as the sections of a pipe might be. As the plant grows, new vascular tissue differentiates in the growing tips of the plant. The new tissue is aligned with existing vascular tissue, maintaining its connection throughout the plant. The ...
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Dianthus
''Dianthus'' () is a genus of about 340 species of flowering plants in the family Caryophyllaceae, native mainly to Europe and Asia, with a few species in north Africa and in southern Africa, and one species (''D. repens'') in arctic North America. Common names include carnation (''D. caryophyllus''), pink (''D. plumarius'' and related species) and sweet william (''D. barbatus''). Description The species are mostly herbaceous perennials, a few are annual or biennial, and some are low subshrubs with woody basal stems. The leaves are opposite, simple, mostly linear and often strongly glaucous grey green to blue green. The flowers have five petals, typically with a frilled or pinked margin, and are (in almost all species) pale to dark pink. One species, ''D. knappii'', has yellow flowers with a purple centre. Some species, particularly the perennial pinks, are noted for their strong spicy fragrance. Species Selected species include: Hybrids include; * 'Devon Xera' – Fir ...
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Dianthus Barbatus
''Dianthus'' () is a genus of about 340 species of flowering plants in the family Caryophyllaceae, native mainly to Europe and Asia, with a few species in north Africa and in southern Africa, and one species (''D. repens'') in arctic North America. Common names include carnation (''D. caryophyllus''), pink (''D. plumarius'' and related species) and sweet william (''D. barbatus''). Description The species are mostly herbaceous perennials, a few are annual or biennial, and some are low subshrubs with woody basal stems. The leaves are opposite, simple, mostly linear and often strongly glaucous grey green to blue green. The flowers have five petals, typically with a frilled or pinked margin, and are (in almost all species) pale to dark pink. One species, ''D. knappii'', has yellow flowers with a purple centre. Some species, particularly the perennial pinks, are noted for their strong spicy fragrance. Species Selected species include: Hybrids include; * 'Devon Xera' – Fir ...
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Immunofluorescence
Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on microbiological samples. This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets within a cell, and therefore allows visualization of the distribution of the target molecule through the sample. The specific region an antibody recognizes on an antigen is called an epitope. There have been efforts in epitope mapping since many antibodies can bind the same epitope and levels of binding between antibodies that recognize the same epitope can vary. Additionally, the binding of the fluorophore to the antibody itself cannot interfere with the immunological specificity of the antibody or the binding capacity of its antigen. Immunofluorescence is a widely used example of immunostaining (using antibodies to stain proteins) and is a specific example of immunohistochemistry (the use of the antibody-antigen rel ...
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Loop-mediated Isothermal Amplification
Loop-mediated isothermal amplification (LAMP) is a single-tube technique for the amplification of DNA and a low-cost alternative to detect certain diseases. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) combines LAMP with a reverse transcription step to allow the detection of RNA. LAMP is an isothermal nucleic acid amplification technique. In contrast to the polymerase chain reaction (PCR) technology, in which the reaction is carried out with a series of alternating temperature steps or cycles, isothermal amplification is carried out at a constant temperature, and does not require a thermal cycler. Technique In LAMP, the target sequence is amplified at a constant temperature of 60–65 °C (140-149 °F) using either two or three sets of primers and a polymerase with high strand displacement activity in addition to a replication activity. Typically, 4 different primers are used to amplify 6 distinct regions on the target gene, which increases ...
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Pink Carnation Crop Two
Pink is the color of a namesake flower that is a pale tint of red. It was first used as a color name in the late 17th century. According to surveys in Europe and the United States, pink is the color most often associated with charm, politeness, sensitivity, tenderness, sweetness, childhood, femininity, and romance. A combination of pink and white is associated with chastity and innocence, whereas a combination of pink and black links to eroticism and seduction. In the 21st century, pink is seen as a symbol of femininity, though this has not always been true; in the 1920s, pink was seen as a color that reflected masculinity. In nature and culture File:Color icon pink v2.svg, Various shades of pink File:Dianthus.jpg, The color pink takes its name from the flowers called pinks, members of the genus ''Dianthus''. File:Rosa Queen Elizabeth1ZIXIETTE.jpg, In most European languages, pink is called ''rose'' or ''rosa'', after the rose flower. File:Cherry blossoms in the Tsu ...
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Rhizosphere
The rhizosphere is the narrow region of soil or substrate that is directly influenced by root secretions and associated soil microorganisms known as the root microbiome. Soil pores in the rhizosphere can contain many bacteria and other microorganisms that feed on sloughed-off plant cells, termed ''rhizodeposition'', and the proteins and sugars released by roots, termed root exudates. This symbiosis leads to more complex interactions, influencing plant growth and competition for resources. Much of the nutrient cycling and disease suppression by antibiotics required by plants, occurs immediately adjacent to roots due to root exudates and metabolic products of symbiotic and pathogenic communities of microorganisms. The rhizosphere also provides space to produce allelochemicals to control neighbours and relatives. The ''rhizoplane'' refers to the root surface including its associated soil particles which closely interact with each other. The plant-soil feedback loop and other p ...
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European And Mediterranean Plant Protection Organization
The European and Mediterranean Plant Protection Organization (EPPO) is an intergovernmental organisation responsible for European cooperation in plant protection in the European and Mediterranean region. Founded on April 18th, 1951 and based in Paris, France, EPPO is the Regional Plant Protection Organization (RPPO) for Europe under the International Plant Protection Convention (IPPC). To meet its objectives to protect plants, strategize against the introduction and spread of dangerous pests, and to promote safe and effective control methods, EPPO develops international standards and recommendations, provides reporting services, participates in global discussions on plant health, EPPO hold regular expert working groups, and maintained EPPO codes. History Founded in 1951 and based in Paris, France, EPPO is the Regional Plant Protection Organization (RPPO) for Europe under the International Plant Protection Convention (IPPC). The intergovernmental organisation responsible for Eu ...
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Cut Flowers
Cut flowers are flowers or flower buds (often with some stem and leaf) that have been cut from the plant bearing it. It is usually removed from the plant for decorative use. Typical uses are in vase displays, wreaths and garlands. Many gardeners harvest their own cut flowers from domestic gardens, but there is a significant floral industry for cut flowers in most countries. The plants cropped vary by climate, culture and the level of wealth locally. Often the plants are raised specifically for the purpose, in field or glasshouse growing conditions. Cut flowers can also be harvested from the wild. The cultivation and trade of flowers is a specialization in horticulture, specifically floriculture. Cultivation Cut flower cultivation is intensive, usually on the basis of greenhouse monocultures, and requires large amounts of highly toxic pesticides, residues of which can often still be found in flower shops on imported flowers. These facts have spurred the development of movem ...
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Bacterial Plant Pathogens And Diseases
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in symbiotic and parasitic relationsh ...
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