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Bromelin
Bromelain is an enzyme extract derived from the plant stem, stems of pineapples, although it exists in all parts of the fresh pineapple. The extract has a history of folk medicine use. As an ingredient, it is used in cosmetics, as a topical medication, and as a meat tenderizer. The term "bromelain" may refer to either of two protease enzymes extracted from the plants of the family Bromeliaceae, or it may refer to a combination of those enzymes along with other compounds produced in an extract. Bromelain enzymes are called ''fruit bromelain'' and ''stem bromelain''. Although tested in a variety of folk medicine and research models for its possible efficacy against diseases, bromelain has only one approved clinical significance, clinical application issued in 2012 by the European Medicines Agency a topical medication called ''NexoBrid'', used to remove dead tissue in severe skin burns. Extract components Bromelain extract is a mixture of protein-digesting (proteolysis, proteolyti ...
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Fruit Bromelain
Fruit bromelain (, ''juice bromelain'', ''ananase'', ''Bromelase'' (a trademark), ''bromelin'', ''extranase'', ''pinase'', ''pineapple enzyme'', ''traumanase'', ''fruit bromelain FA2'') is an enzyme. This enzyme catalysis, catalyses the following chemical reaction : Hydrolysis of proteins with broad specificity for peptide bonds. Bz-Phe-Val-Arg-NHMec is a good synthetic substrate This enzyme is isolated from pineapple plant, ''Ananas comosus''. See also * Bromelain References External links

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Enzyme
Enzymes () are proteins that act as biological catalysts by accelerating chemical reactions. The molecules upon which enzymes may act are called substrates, and the enzyme converts the substrates into different molecules known as products. Almost all metabolic processes in the cell need enzyme catalysis in order to occur at rates fast enough to sustain life. Metabolic pathways depend upon enzymes to catalyze individual steps. The study of enzymes is called ''enzymology'' and the field of pseudoenzyme analysis recognizes that during evolution, some enzymes have lost the ability to carry out biological catalysis, which is often reflected in their amino acid sequences and unusual 'pseudocatalytic' properties. Enzymes are known to catalyze more than 5,000 biochemical reaction types. Other biocatalysts are catalytic RNA molecules, called ribozymes. Enzymes' specificity comes from their unique three-dimensional structures. Like all catalysts, enzymes increase the reaction ra ...
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Stem Bromelain
Stem bromelain (SBM) ( EC 3.4.22.32), a proteolytic enzyme, is a widely accepted phytotherapeutical drug member of the bromelain family of proteolytic enzymes obtained from ''Ananas comosus''. Some of the therapeutic uses of SBM are reversible inhibition of platelet aggregation, angina pectoris Angina, also known as angina pectoris, is chest pain or pressure, usually caused by insufficient blood flow to the heart muscle (myocardium). It is most commonly a symptom of coronary artery disease. Angina is typically the result of obstru ..., bronchitis, sinusitis, surgical traumas, thrombophlebitis, pyelonephritis and enhanced Absorption (pharmacokinetics), absorption of drugs, particularly of antibiotics. Its anti-metastasis and anti-inflammatory activities are apparently independent of its proteolytic activity. Although poorly understood, the diverse Pleiotrophy, pleiotrophic effects of SBM seem to depend on its ability to traverse the membrane barrier, a very unusual propert ...
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Kiwi Fruit
Kiwifruit (often shortened to kiwi in North American, British and continental European English) or Chinese gooseberry is the edible berry of several species of woody vines in the genus ''Actinidia''. The most common cultivar group of kiwifruit (''Actinidia deliciosa'' 'Hayward') is oval, about the size of a large hen's egg: in length and in diameter. It has a thin, fuzzy, fibrous, tart but edible light brown skin and light green or golden flesh with rows of tiny, black, edible seeds. The fruit has a soft texture with a sweet and unique flavour. Kiwifruit is native to central and eastern China. The first recorded description of the kiwifruit dates to the 12th century during the Song dynasty. In the early 20th century, cultivation of kiwifruit spread from China to New Zealand, where the first commercial plantings occurred. The fruit became popular with British and American servicemen stationed in New Zealand during World War II, and later became commonly exported, first to G ...
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Actinidin
Actinidain (, ''actinidin'', ''Actinidia anionic protease'', ''proteinase A2 of Actinidia chinensis'') is a type of cysteine protease enzyme found in fruits including kiwifruit (genus '' Actinidia''), pineapple, mango, banana, figs, and papaya. This enzyme is part of the peptidase C1 family of papain-like proteases. As a known allergen in kiwifruit, the enzyme is under preliminary research for its effect on tight junction proteins of intestinal epithelial cells. Actinidain is commercially useful as a meat tenderiser and in coagulating milk for dairy products. The denaturation temperature of actinidain is , lower than that of similar meat tenderising enzymes bromelain from pineapple and papain from papaya. References External links * The MEROPS MEROPS is an online database for peptidases (also known as proteases, proteinases and proteolytic enzymes) and their inhibitors. The classification scheme for peptidases was published by Rawlings & Barrett in 1993, and that for ...
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Papaya
The papaya (, ), papaw, () or pawpaw () is the plant species ''Carica papaya'', one of the 21 accepted species in the genus ''Carica'' of the family Caricaceae. It was first domesticated in Mesoamerica, within modern-day southern Mexico and Central America. In 2020, India produced 43% of the world supply of papayas. Etymology The word ''papaya'' comes from Arawak via Spanish, this is also where ''papaw'' and ''pawpaw'' come from. Description The papaya is a small, sparsely branched tree, usually with a single stem growing from tall, with spirally arranged leaves confined to the top of the trunk. The lower trunk is conspicuously scarred where leaves and fruit were borne. The leaves are large, in diameter, deeply palmately lobed, with seven lobes. All parts of the plant contain latex in articulated laticifers. Flowers Papayas are dioecious. The flowers are five-parted and highly dimorphic; the male flowers have the stamens fused to the petals. The female flowers h ...
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Papain
Papain, also known as papaya proteinase I, is a cysteine protease () enzyme present in papaya (''Carica papaya'') and mountain papaya (''Vasconcellea cundinamarcensis''). It is the namesake member of the papain-like protease family. It has wide ranging commercial applications in the leather, cosmetic, textiles, detergents, food and pharmaceutical industries. In the food industry, papain is used as an active ingredient in many commercial meat tenderizers. Papain family Papain belongs to a family of related proteins, known as the papain-like protease family, with a wide variety of activities, including endopeptidases, aminopeptidases, dipeptidyl peptidases and enzymes with both exo- and endopeptidase activity. Members of the papain family are widespread, found in Baculoviridae, baculoviruses, eubacteria, yeast, and practically all protozoa, plants and mammals. The proteins are typically lysosomal or secreted, and proteolytic cleavage of the propeptide is required for enzyme activa ...
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Supernatant
In an aqueous solution, precipitation is the process of transforming a dissolved substance into an insoluble solid from a super-saturated solution. The solid formed is called the precipitate. In case of an inorganic chemical reaction leading to precipitation, the chemical reagent causing the solid to form is called the ''precipitant''. The clear liquid remaining above the precipitated or the centrifuged solid phase is also called the 'supernate' or 'supernatant'. The notion of precipitation can also be extended to other domains of chemistry (organic chemistry and biochemistry) and even be applied to the solid phases (''e.g.'', metallurgy and alloys) when solid impurities segregate from a solid phase. Supersaturation The precipitation of a compound may occur when its concentration exceeds its solubility. This can be due to temperature changes, solvent evaporation, or by mixing solvents. Precipitation occurs more rapidly from a strongly supersaturated solution. The formati ...
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Extraction (chemistry)
Extraction in chemistry is a separation process consisting of the separation of a substance from a matrix. Common examples include '' liquid-liquid extraction'', and ''solid phase extraction''. The distribution of a solute between two phases is an equilibrium condition described by partition theory. This is based on exactly how the analyte moves from the initial solvent into the extracting solvent. The term ''washing'' may also be used to refer to an extraction in which impurities are extracted from the solvent containing the desired compound. Types of extraction * Liquid–liquid extraction * Solid-phase extraction * Acid-base extraction * Supercritical fluid extraction * Ultrasound-assisted extraction * Heat reflux extraction * Mechanochemical-assisted extraction * Maceration * Microwave-assisted extraction * Instant controlled pressure drop extraction (DIC, from the French, Détente instantanée contrôlée) * Perstraction Laboratory applications and examples Liquid ...
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Malaysia
Malaysia ( ; ) is a country in Southeast Asia. The federation, federal constitutional monarchy consists of States and federal territories of Malaysia, thirteen states and three federal territories, separated by the South China Sea into two regions: Peninsular Malaysia and Borneo's East Malaysia. Peninsular Malaysia shares a land and maritime Malaysia–Thailand border, border with Thailand and Maritime boundary, maritime borders with Singapore, Vietnam, and Indonesia. East Malaysia shares land and maritime borders with Brunei and Indonesia, and a maritime border with the Philippines and Vietnam. Kuala Lumpur is the national capital, the country's largest city, and the seat of the Parliament of Malaysia, legislative branch of the Government of Malaysia, federal government. The nearby Planned community#Planned capitals, planned capital of Putrajaya is the administrative capital, which represents the seat of both the Government of Malaysia#Executive, executive branch (the Cabine ...
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Thailand
Thailand ( ), historically known as Siam () and officially the Kingdom of Thailand, is a country in Southeast Asia, located at the centre of the Indochinese Peninsula, spanning , with a population of almost 70 million. The country is bordered to the north by Myanmar and Laos, to the east by Laos and Cambodia, to the south by the Gulf of Thailand and Malaysia, and to the west by the Andaman Sea and the extremity of Myanmar. Thailand also shares maritime borders with Vietnam to the southeast, and Indonesia and India to the southwest. Bangkok is the nation's capital and largest city. Tai peoples migrated from southwestern China to mainland Southeast Asia from the 11th century. Indianised kingdoms such as the Mon, Khmer Empire and Malay states ruled the region, competing with Thai states such as the Kingdoms of Ngoenyang, Sukhothai, Lan Na and Ayutthaya, which also rivalled each other. European contact began in 1511 with a Portuguese diplomatic mission to Ayutthaya, w ...
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Extract
An extract is a substance made by extracting a part of a raw material, often by using a solvent such as ethanol, oil or water. Extracts may be sold as tinctures, absolutes or in powder form. The aromatic principles of many spices, nuts, herbs, fruits, etc., and some flowers, are marketed as extracts, among the best known of true extracts being almond, cinnamon, cloves, ginger, lemon, nutmeg, orange, peppermint, pistachio, rose, spearmint, vanilla, violet, rum, and wintergreen. Extraction techniques Most natural essences are obtained by extracting the essential oil from the feedstock, such as blossoms, fruit, and roots, or from intact plants through multiple techniques and methods: * Expression (juicing, pressing) involves physical extraction material from feedstock, used when the oil is plentiful and easily obtained from materials such as citrus peels, olives, and grapes. * Absorption (steeping, decoction). Extraction is done by soaking material in a solvent, as ...
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