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Zymoblot
Zymoblot is the fastest available microtechnique to detect gene expression or enzyme activity in any biological specimen. The technique was invented by Professor Elsayed Elsayed Wagih in collaboration with Professor Jacqueline Fletcher of the Department of Plant Pathology, Noble Research Centre, Oklahoma State University, US in 1993.Wagih, E. E. and Fletcher, J. (1993). Zymoblot, a new microtechnique used to detect enzyme activity in spiroplasmas and bacteria. Canadian Journal of Microbiology 39: 543- 547. Background Physiological phenomena whether at the cellular or molecular level in living organisms are driven either directly or indirectly by enzyme reactions. The assay of enzyme activities in living organisms is therefore one of the most commonly performed activities in modern physiology laboratories. Numerous methods of enzyme assay Enzyme assays are laboratory methods for measuring enzymatic activity. They are vital for the study of enzyme kinetics and enzyme inhibition. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Elsayed Elsayed Wagih
Elsayed Elsayed Wagih PhD, DIC, CIDTT (born 21 November 1946) is an Egyptian professor of virology and biotechnology and vice President of the Arab Society for Biotechnology. He was born in Alexandria, Egypt. Wagih is well known for having invented Zymoblot, the fastest available microtechnique to detect gene expression and/or enzyme activity in any biological specimen as well as the ”Mirror Image ''in vivo'' electro-blotting technique” that detects virus particles or any foreign protein (Gene Expression) in any tissue. He also discovered two viruses reported under his name in the world data bank of viruses, the first was named "Peanut Chlorotic Ringspot Virus (PCRV)" and the second was called “Peanut Top Paralysis (PTPV)". Also, a champion in Greek-Roman and free-style wrestling in Egypt for the period from 1965 to 1977. He was the one who made the College of Agriculture enjoy, for the first time at the level of University of Alexandria, the winning, in 1965–1966, of fi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Microtechnique
Microtechnique is an aggregate of methods used to prepare micro-objects for studying. It is currently being employed in many fields in life science. Two well-known branches of microtechnique are botanical (plant) microtechnique and zoological (animal) microtechnique. With respect to both plant microtechnique and animal microtechnique, four types of methods are commonly used, which are whole mounts, smears, squashes, and sections, in recent micro experiments. Plant microtechnique contains direct macroscopic examinations, freehand sections, clearing, maceration, embedding, and staining. Moreover, three preparation ways used in zoological micro observations are paraffin method, celloidin method, and freezing method. History The early development of microtechnique in botany is closely related to that in zoology. Zoological and botanical discoveries are adopted by both zoologists and botanists. The field of microtechnique lasted from at the end of the 1930s when the principle of dry p ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sequential Order
In mathematics, a sequence is an enumerated collection of objects in which repetitions are allowed and order matters. Like a set, it contains members (also called ''elements'', or ''terms''). The number of elements (possibly infinite) is called the ''length'' of the sequence. Unlike a set, the same elements can appear multiple times at different positions in a sequence, and unlike a set, the order does matter. Formally, a sequence can be defined as a function from natural numbers (the positions of elements in the sequence) to the elements at each position. The notion of a sequence can be generalized to an indexed family, defined as a function from an ''arbitrary'' index set. For example, (M, A, R, Y) is a sequence of letters with the letter 'M' first and 'Y' last. This sequence differs from (A, R, M, Y). Also, the sequence (1, 1, 2, 3, 5, 8), which contains the number 1 at two different positions, is a valid sequence. Sequences can be ''finite'', as in these examples, or ''infinite ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
