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Somatic Embryogenesis
Somatic embryogenesis is an artificial process in which a plant or embryo is derived from a single somatic cell. Somatic embryos are formed from plant cells that are not normally involved in the development of embryos, i.e. ordinary plant tissue. No endosperm or seed coat is formed around a somatic embryo. Cells derived from competent source tissue are cultured to form an undifferentiated mass of cells called a callus. Plant growth regulators in the tissue culture medium can be manipulated to induce callus formation and subsequently changed to induce embryos to form the callus. The ratio of different plant growth regulators required to induce callus or embryo formation varies with the type of plant. Somatic embryos are mainly produced ''in vitro'' and for laboratory purposes, using either solid or liquid nutrient media which contain plant growth regulators (PGR’s). The main PGRs used are auxins but can contain cytokinin in a smaller amount.E.F. George et al. (eds.), Plant Propaga ...
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Conger Type Callus 3ms White Light
''Conger'' ( ) is a genus of marine congrid eels. It includes some of the largest types of eels, ranging up to 2 m (6 ft) or more in length, in the case of the European conger. Large congers have often been observed by divers during the day in parts of the Mediterranean Sea, and both European and American congers are sometimes caught by fishermen along the European and North American Atlantic coasts. The life histories of most conger eels are poorly known. Based on collections of their small leptocephalus larvae, the American conger eel has been found to spawn in the southwestern Sargasso Sea, close to the spawning areas of the Atlantic freshwater eels. "Conger" or "conger eel" is sometimes included in the common names of species of the family Congridae, including members of this genus. Description Congers have wide mouths with sturdy teeth, usually a variant of gray or black in coloration. They have no scales. Their body weight can reach over 57 kilograms (125 lbs ...
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Shoot
In botany, a plant shoot consists of any plant stem together with its appendages, leaves and lateral buds, flowering stems, and flower buds. The new growth from seed germination that grows upward is a shoot where leaves will develop. In the spring, perennial plant shoots are the new growth that grows from the ground in herbaceous plants or the new stem or flower growth that grows on woody plants. In everyday speech, shoots are often synonymous with stems. Stems, which are an integral component of shoots, provide an axis for buds, fruits, and leaves. Young shoots are often eaten by animals because the fibers in the new growth have not yet completed secondary cell wall development, making the young shoots softer and easier to chew and digest. As shoots grow and age, the cells develop secondary cell walls that have a hard and tough structure. Some plants (e.g. bracken) produce toxins that make their shoots inedible or less palatable. File:Cucumber leaf.jpg, The shoot of a cucu ...
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Woody Plant
A woody plant is a plant that produces wood as its structural tissue and thus has a hard stem. In cold climates, woody plants further survive winter or dry season above ground, as opposite to herbaceous plants that die back to the ground until spring. Characteristics Woody plants are usually either trees, shrubs, or lianas. These are usually perennial plants whose stems and larger roots are reinforced with wood produced from secondary xylem. The main stem, larger branches, and roots of these plants are usually covered by a layer of bark. Wood is a structural tissue that allows woody plants to grow from above ground stems year after year, thus making some woody plants the largest and tallest terrestrial plants. Woody plants, like herbaceous perennials, typically have a dormant period of the year when growth does not take place, in colder climates due to freezing temperatures and lack of daylight during the winter months, in subtropical and tropical climates due to the dry sea ...
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Callus (cell Biology)
Plant callus (plural ''calluses'' or ''calli'') is a growing mass of unorganized plant parenchyma cells. In living plants, callus cells are those cells that cover a plant wound. In biological research and biotechnology callus formation is induced from plant tissue samples (explants) after surface sterilization and plating onto tissue culture medium in vitro (in a closed culture vessel such as a Petri dish). The culture medium is supplemented with plant growth regulators, such as auxin, cytokinin, and gibberellin, to initiate callus formation or somatic embryogenesis. Callus initiation has been described for all major groups of land plants. Callus induction and tissue culture Plant species representing all major land plant groups have been shown to be capable of producing callus in tissue culture. A callus cell culture is usually sustained on gel medium. Callus induction medium consists of agar and a mixture of macronutrients and micronutrients for the given cell type. ...
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Plant Propagation
Plant propagation is the process by which new plants grow from a variety of sources: seeds, cuttings, and other plant parts. Plant propagation can also refer to the man-made or natural dispersal of seeds. Propagation typically occurs as a step in the overall cycle of plant growth. For seeds, it happens after ripening and dispersal; for vegetative parts, it happens after detachment or pruning; for asexually-reproducing plants, such as strawberry, it happens as the new plant develops from existing parts. Plant propagation can be divided into four basic types: sexual, asexual (vegetative), layering, and grafting. Countless plants are propagated each day in horticulture and agriculture. The materials commonly used for plant propagation are seeds and cuttings. Sexual propagation Seeds and spores can be used for reproduction (e.g. sowing). Seeds are typically produced from sexual reproduction within a species because genetic recombination has occurred. A plant grown from seeds may ...
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Transformation (genetics)
In molecular biology and genetics, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane(s). For transformation to take place, the recipient bacterium must be in a state of competence, which might occur in nature as a time-limited response to environmental conditions such as starvation and cell density, and may also be induced in a laboratory. Transformation is one of three processes that lead to horizontal gene transfer, in which exogenous genetic material passes from one bacterium to another, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium). In transformation, the genetic material passes through the intervening medium, and uptake is completely dependent on the recipient bacterium. As of 2014 about 8 ...
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Protoplast
Protoplast (), is a biological term coined by Hanstein in 1880 to refer to the entire cell, excluding the cell wall. Protoplasts can be generated by stripping the cell wall from plant, bacterial, or fungal cells by mechanical, chemical or enzymatic means. Protoplasts differ from spheroplasts in that their cell wall has been completely removed. Spheroplasts retain part of their cell wall. In the case of Gram-negative bacterial spheroplasts, for example, the peptidoglycan component of the cell wall has been removed but the outer membrane component has not. Enzymes for the preparation of protoplasts Cell walls are made of a variety of polysaccharides. Protoplasts can be made by degrading cell walls with a mixture of the appropriate polysaccharide-degrading enzymes: During and subsequent to digestion of the cell wall, the protoplast becomes very sensitive to osmotic stress. This means cell wall digestion and protoplast storage must be done in an isotonic solution to prevent ...
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Transformation (genetics)
In molecular biology and genetics, transformation is the genetic alteration of a cell resulting from the direct uptake and incorporation of exogenous genetic material from its surroundings through the cell membrane(s). For transformation to take place, the recipient bacterium must be in a state of competence, which might occur in nature as a time-limited response to environmental conditions such as starvation and cell density, and may also be induced in a laboratory. Transformation is one of three processes that lead to horizontal gene transfer, in which exogenous genetic material passes from one bacterium to another, the other two being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of foreign DNA by a bacteriophage virus into the host bacterium). In transformation, the genetic material passes through the intervening medium, and uptake is completely dependent on the recipient bacterium. As of 2014 about 8 ...
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Virus
A virus is a submicroscopic infectious agent that replicates only inside the living cells of an organism. Viruses infect all life forms, from animals and plants to microorganisms, including bacteria and archaea. Since Dmitri Ivanovsky's 1892 article describing a non-bacterial pathogen infecting tobacco plants and the discovery of the tobacco mosaic virus by Martinus Beijerinck in 1898,Dimmock p. 4 more than 9,000 virus species have been described in detail of the millions of types of viruses in the environment. Viruses are found in almost every ecosystem on Earth and are the most numerous type of biological entity. The study of viruses is known as virology, a subspeciality of microbiology. When infected, a host cell is often forced to rapidly produce thousands of copies of the original virus. When not inside an infected cell or in the process of infecting a cell, viruses exist in the form of independent particles, or ''virions'', consisting of (i) the genetic material, i. ...
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Clonal Propagation
Vegetative reproduction (also known as vegetative propagation, vegetative multiplication or cloning) is any form of asexual reproduction occurring in plants in which a new plant grows from a fragment or cutting of the parent plant or specialized reproductive structures, which are sometimes called vegetative propagules. Many plants naturally reproduce this way, but it can also be induced artificially. Horticulturists have developed asexual propagation techniques that use vegetative propagules to replicate plants. Success rates and difficulty of propagation vary greatly. Monocotyledons typically lack a vascular cambium, making them more challenging to propagate. Background Plant propagation is the process of plant reproduction of a species or cultivar, and it can be sexual or asexual. It can happen through the use of vegetative parts of the plants, such as leaves, stems, and roots to produce new plants or through growth from specialized vegetative plant parts. While man ...
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Signaling Molecules
In biology, cell signaling (cell signalling in British English) or cell communication is the ability of a cell to receive, process, and transmit signals with its environment and with itself. Cell signaling is a fundamental property of all cellular life in prokaryotes and eukaryotes. Signals that originate from outside a cell (or extracellular signals) can be physical agents like mechanical pressure, voltage, temperature, light, or chemical signals (e.g., small molecules, peptides, or gas). Cell signaling can occur over short or long distances, and as a result can be classified as autocrine, juxtacrine, intracrine, paracrine, or endocrine. Signaling molecules can be synthesized from various biosynthetic pathways and released through passive or active transports, or even from cell damage. Receptors play a key role in cell signaling as they are able to detect chemical signals or physical stimuli. Receptors are generally proteins located on the cell surface or within the interi ...
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Vitamin
A vitamin is an organic molecule (or a set of molecules closely related chemically, i.e. vitamers) that is an Nutrient#Essential nutrients, essential micronutrient that an organism needs in small quantities for the proper functioning of its metabolism. Essential nutrients cannot be biosynthesis, synthesized in the organism, either at all or not in sufficient quantities, and therefore must be obtained through the Diet (nutrition), diet. Vitamin C can be synthesized by some species but not by others; it is not a vitamin in the first instance but is in the second. The term ''vitamin'' does not include the three other groups of essential nutrients: mineral (nutrient), minerals, essential fatty acids, and essential amino acids. Most vitamins are not single molecules, but groups of related molecules called vitamers. For example, there are eight vitamers of vitamin E: four tocopherols and four tocotrienols. Some sources list fourteen vitamins, by including choline, but major health ...
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