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Seed Testing
Seed testing is performed for a number of reasons, including research purposes or to determine if seed storage techniques are functioning. There are four tests most commonly done. The first two listed below are common for scientific research. For commercially sold seed, all four of these tests are done in dedicated laboratories by trained and usually certified analysts. The tests are designed to evaluate the quality of the seed lot being sold. * Germination test : Reports the percentage of seed that germinated. In commercial settings, tests are usually made in either 200 or 400 seed samples. * Viability test (TZ test): A test for viability that involves three steps: ** 1. preconditioning (imbibition) ** 2. preparation and staining (sometimes cutting the seed and then soaking the seed in a 2,3,5 triphenyl tetrazolium chloride solution) *** Tetrazolim chloride seed testing: The Tetrazolium Chloride (TZ) test is often called the quick germination test. It's a chemical test used to ...
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Laboratories
A laboratory (; ; colloquially lab) is a facility that provides controlled conditions in which scientific or technological research, experiments, and measurement may be performed. Laboratory services are provided in a variety of settings: physicians' offices, clinics, hospitals, and regional and national referral centers. Overview The organisation and contents of laboratories are determined by the differing requirements of the specialists working within. A physics laboratory might contain a particle accelerator or vacuum chamber, while a metallurgy laboratory could have apparatus for casting or refining metals or for testing their strength. A chemist or biologist might use a wet laboratory, while a psychologist's laboratory might be a room with one-way mirrors and hidden cameras in which to observe behavior. In some laboratories, such as those commonly used by computer scientists, computers (sometimes supercomputers) are used for either simulations or the analysis of data. Scienti ...
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Seed
A seed is an embryonic plant enclosed in a protective outer covering, along with a food reserve. The formation of the seed is a part of the process of reproduction in seed plants, the spermatophytes, including the gymnosperm and angiosperm plants. Seeds are the product of the ripened ovule, after the embryo sac is fertilized by sperm from pollen, forming a zygote. The embryo within a seed develops from the zygote, and grows within the mother plant to a certain size before growth is halted. The seed coat arises from the integuments of the ovule. Seeds have been an important development in the reproduction and success of vegetable gymnosperm and angiosperm plants, relative to more primitive plants such as ferns, mosses and liverworts, which do not have seeds and use water-dependent means to propagate themselves. Seed plants now dominate biological niches on land, from forests to grasslands both in hot and cold climates. The term "seed" also has a general me ...
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Germination
Germination is the process by which an organism grows from a seed or spore. The term is applied to the sprouting of a seedling from a seed of an angiosperm or gymnosperm, the growth of a sporeling from a spore, such as the spores of fungi, ferns, bacteria, and the growth of the pollen tube from the pollen grain of a seed plant. Seed plants Germination is usually the growth of a plant contained within a seed; it results in the formation of the seedling. It is also the process of reactivation of metabolic machinery of the seed resulting in the emergence of radicle and plumule. The seed of a vascular plant is a small package produced in a fruit or cone after the union of male and female reproductive cells. All fully developed seeds contain an embryo and, in most plant species some store of food reserves, wrapped in a seed coat. Some plants produce varying numbers of seeds that lack embryos; these are empty seeds which never germinate. Dormant seeds are viable seeds that do ...
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Tetrazolium Chloride
Triphenyl tetrazolium chloride, TTC, or simply tetrazolium chloride (with the formula 2,3,5-triphenyl-2H-tetrazolium chloride) is a redox indicator commonly used in biochemical experiments especially to indicate cellular respiration (example: to check for the viability of seeds). It is a white crystalline salt, soluble in water, ethanol and acetone but insoluble in ether. TTC assay In the TTC assay (also known as TTC test or tetrazolium test), TTC is used to differentiate between metabolically active and inactive tissues. The white compound is enzymatically reduced to red TPF (1,3,5-triphenylformazan) in living tissues due to the activity of various dehydrogenases (enzymes important in oxidation of organic compounds and thus cellular metabolism), while it remains in its unreacted state in areas of necrosis since these enzymes have either denatured or degraded. TTC has been employed in autopsy pathology to identify myocardial infarctions. Healthy viable heart muscle will stain dee ...
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Embryo
An embryo is an initial stage of development of a multicellular organism. In organisms that reproduce sexually, embryonic development is the part of the life cycle that begins just after fertilization of the female egg cell by the male sperm cell. The resulting fusion of these two cells produces a single-celled zygote that undergoes many cell divisions that produce cells known as blastomeres. The blastomeres are arranged as a solid ball that when reaching a certain size, called a morula, takes in fluid to create a cavity called a blastocoel. The structure is then termed a blastula, or a blastocyst in mammals. The mammalian blastocyst hatches before implantating into the endometrial lining of the womb. Once implanted the embryo will continue its development through the next stages of gastrulation, neurulation, and organogenesis. Gastrulation is the formation of the three germ layers that will form all of the different parts of the body. Neurulation forms the nervous ...
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Weed
A weed is a plant considered undesirable in a particular situation, "a plant in the wrong place", or a plant growing where it is not wanted.Harlan, J. R., & deWet, J. M. (1965). Some thoughts about weeds. ''Economic botany'', ''19''(1), 16-24. This introduces the concept of humans and their goals in a particular setting.Holzner, W., & Numata, M. (Eds.). (2013). ''Biology and ecology of weeds'' (Vol. 2). Springer Science & Business Media. The concept of weeds is particularly significant in agriculture, where the aim is growing crops or pastures of a single species, or a mixture of a few desired species. In such environments, other plant species are considered undesirable and therefore a weed. Besides, some weeds have undesirable characteristics making them a plant pest in most human settings.Harlan, J. R., & deWet, J. M. (1965). Some thoughts about weeds. ''Economic botany'', ''19''(1), 16-24.Holzner, W., & Numata, M. (Eds.). (2013). ''Biology and ecology of weeds'' (Vol. 2). Spri ...
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