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Sarfus
Sarfus is an optical quantitative imaging technique based on the association of: *an upright or inverted optical microscope in crossed polarization configuration and *specific supporting plates – called surfs – on which the sample to observe is deposited. Sarfus visualization is based on the perfect control of the reflection properties of polarized light on a surface, which leads to an increase in the axial sensitivity of optical microscope by a factor of around 100 without reducing its lateral resolution. Thus this new technique increases the sensitivity of standard optical microscope to a point that it becomes possible to directly visualize thin films (down to 0.3 micrometer) and isolated nano-objects in real-time, be it in air or in water. Principles A recent study on polarized light coherence leads to the development of new supports – the surfs – having contrast amplification properties for standard optical microscopy in cross polarizers mode. Made of optical layers ...
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Sarfus
Sarfus is an optical quantitative imaging technique based on the association of: *an upright or inverted optical microscope in crossed polarization configuration and *specific supporting plates – called surfs – on which the sample to observe is deposited. Sarfus visualization is based on the perfect control of the reflection properties of polarized light on a surface, which leads to an increase in the axial sensitivity of optical microscope by a factor of around 100 without reducing its lateral resolution. Thus this new technique increases the sensitivity of standard optical microscope to a point that it becomes possible to directly visualize thin films (down to 0.3 micrometer) and isolated nano-objects in real-time, be it in air or in water. Principles A recent study on polarized light coherence leads to the development of new supports – the surfs – having contrast amplification properties for standard optical microscopy in cross polarizers mode. Made of optical layers ...
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Sarfus LBLayers
Sarfus is an optical quantitative imaging technique based on the association of: *an upright or inverted optical microscope in crossed polarization configuration and *specific supporting plates – called surfs – on which the sample to observe is deposited. Sarfus visualization is based on the perfect control of the reflection properties of polarized light on a surface, which leads to an increase in the axial sensitivity of optical microscope by a factor of around 100 without reducing its lateral resolution. Thus this new technique increases the sensitivity of standard optical microscope The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microsco ... to a point that it becomes possible to directly visualize thin films (down to 0.3 micrometer) and isolated nano-objects in real-time, be it in ai ...
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Sarfus PolarisationState
Sarfus is an optical quantitative imaging technique based on the association of: *an upright or inverted optical microscope in crossed polarization configuration and *specific supporting plates – called surfs – on which the sample to observe is deposited. Sarfus visualization is based on the perfect control of the reflection properties of polarized light on a surface, which leads to an increase in the axial sensitivity of optical microscope by a factor of around 100 without reducing its lateral resolution. Thus this new technique increases the sensitivity of standard optical microscope to a point that it becomes possible to directly visualize thin films (down to 0.3 micrometer) and isolated nano-objects in real-time, be it in air or in water. Principles A recent study on polarized light coherence leads to the development of new supports – the surfs – having contrast amplification properties for standard optical microscopy in cross polarizers mode. Made of optical layers ...
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Sarfus ExamplesVisu
Sarfus is an optical quantitative imaging technique based on the association of: *an upright or inverted optical microscope in crossed polarization configuration and *specific supporting plates – called surfs – on which the sample to observe is deposited. Sarfus visualization is based on the perfect control of the reflection properties of polarized light on a surface, which leads to an increase in the axial sensitivity of optical microscope by a factor of around 100 without reducing its lateral resolution. Thus this new technique increases the sensitivity of standard optical microscope to a point that it becomes possible to directly visualize thin films (down to 0.3 micrometer) and isolated nano-objects in real-time, be it in air or in water. Principles A recent study on polarized light coherence leads to the development of new supports – the surfs – having contrast amplification properties for standard optical microscopy in cross polarizers mode. Made of optical layers ...
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Langmuir–Blodgett Film
A Langmuir–Blodgett (LB) film is a nanostructured system formed when Langmuir films—or Langmuir monolayers (LM)—are transferred from the liquid-gas interface to solid supports during the vertical passage of the support through the monolayers. LB films can contain one or more monolayers of an organic material, deposited from the surface of a liquid onto a solid by immersing (or emersing) the solid substrate into (or from) the liquid. A monolayer is adsorbed homogeneously with each immersion or emersion step, thus films with very accurate thickness can be formed. This thickness is accurate because the thickness of each monolayer is known and can therefore be added to find the total thickness of a Langmuir–Blodgett film. The monolayers are assembled vertically and are usually composed either of amphiphilic molecules (see chemical polarity) with a hydrophilic head and a hydrophobic tail (example: fatty acids) or nowadays commonly of nanoparticles. Langmuir–Blodgett films ar ...
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Biochip
In molecular biology, biochips are engineered substrates ("miniaturized laboratories") that can host large numbers of simultaneous biochemical reactions. One of the goals of biochip technology is to efficiently screen large numbers of biological analytes, with potential applications ranging from disease diagnosis to detection of bioterrorism agents. For example, digital microfluidic biochips are under investigation for applications in biomedical fields. In a digital microfluidic biochip, a group of (adjacent) cells in the microfluidic array can be configured to work as storage, functional operations, as well as for transporting fluid droplets dynamically. History The development started with early work on the underlying sensor technology. One of the first portable, chemistry-based sensors was the glass pH electrode, invented in 1922 by Hughes. The basic concept of using exchange sites to create permselective membranes was used to develop other ion sensors in subsequent years. Fo ...
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Soft Lithography
In technology, soft lithography is a family of techniques for fabricating or replicating structures using "elastomeric stamps, molds, and conformable photomasks". It is called "soft" because it uses elastomeric materials, most notably PDMS. Soft lithography is generally used to construct features measured on the micrometer to nanometer scale. According to Rogers and Nuzzo (2005), development of soft lithography expanded rapidly from 1995 to 2005. Soft lithography tools are now commercially available. Types * PDMS stamp * Microcontact printing * Multilayer soft lithography Advantages Soft lithography has some unique advantages over other forms of lithography (such as photolithography and electron beam lithography). They include the following: *Lower cost than traditional photolithography in mass production *Well-suited for applications in biotechnology *Well-suited for applications in plastic electronics *Well-suited for applications involving large or nonplanar (nonflat) ...
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Optical Microscope
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century. Basic optical microscopes can be very simple, although many complex designs aim to improve resolution and sample contrast. The object is placed on a stage and may be directly viewed through one or two eyepieces on the microscope. In high-power microscopes, both eyepieces typically show the same image, but with a stereo microscope, slightly different images are used to create a 3-D effect. A camera is typically used to capture the image (micrograph). The sample can be lit in a variety of ways. Transparent objects can be lit from below and solid objects can be lit with light coming through ( bright field) or around (dark field) the objective lens. Polarised ...
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Nanoparticles
A nanoparticle or ultrafine particle is usually defined as a particle of matter that is between 1 and 100 nanometres (nm) in diameter. The term is sometimes used for larger particles, up to 500 nm, or fibers and tubes that are less than 100 nm in only two directions. At the lowest range, metal particles smaller than 1 nm are usually called atom clusters instead. Nanoparticles are usually distinguished from microparticles (1-1000 µm), "fine particles" (sized between 100 and 2500 nm), and "coarse particles" (ranging from 2500 to 10,000 nm), because their smaller size drives very different physical or chemical properties, like colloidal properties and ultrafast optical effects or electric properties. Being more subject to the brownian motion, they usually do not sediment, like colloidal particles that conversely are usually understood to range from 1 to 1000 nm. Being much smaller than the wavelengths of visible light (400-700 nm), nano ...
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Biological Techniques And Tools
Biology is the scientific study of life. It is a natural science with a broad scope but has several unifying themes that tie it together as a single, coherent field. For instance, all organisms are made up of cells that process hereditary information encoded in genes, which can be transmitted to future generations. Another major theme is evolution, which explains the unity and diversity of life. Energy processing is also important to life as it allows organisms to move, grow, and reproduce. Finally, all organisms are able to regulate their own internal environments. Biologists are able to study life at multiple levels of organization, from the molecular biology of a cell to the anatomy and physiology of plants and animals, and evolution of populations.Based on definition from: Hence, there are multiple subdisciplines within biology, each defined by the nature of their research questions and the tools that they use. Like other scientists, biologists use the scientific meth ...
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Scientific Techniques
A scientific technique is any systematic way of obtaining information about a scientific nature or to obtain a desired material or product. Scientific techniques can be divided in many different groups, e.g.: # Preparative techniques ## Synthesis techniques, e.g. the use of Grignard reagents in organic chemistry ## Growth techniques, e.g. crystal growth or cell cultures in biology ## Purification techniques e.g. those in chemistry # Measurement techniques ## Analysis techniques, e.g. ones that reveal atomic or molecular composition. ## Characterization techniques, e.g. ones that measure a certain property of a material. ## Imaging techniques, e.g. microscopy In some cases these methods have evolved into instrumental techniques that require expensive equipment. This is particularly true in sciences like physics, chemistry, and astronomy. It is customary to abbreviate the names of techniques into acronyms, although this does not hold for all of them. Particularly the advent of th ...
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Laboratory Techniques
A laboratory (; ; colloquially lab) is a facility that provides controlled conditions in which scientific or technological research, experiments, and measurement may be performed. Laboratory services are provided in a variety of settings: physicians' offices, clinics, hospitals, and regional and national referral centers. Overview The organisation and contents of laboratories are determined by the differing requirements of the specialists working within. A physics laboratory might contain a particle accelerator or vacuum chamber, while a metallurgy laboratory could have apparatus for casting or refining metals or for testing their strength. A chemist or biologist might use a wet laboratory, while a psychologist's laboratory might be a room with one-way mirrors and hidden cameras in which to observe behavior. In some laboratories, such as those commonly used by computer scientists, computers (sometimes supercomputers) are used for either simulations or the analysis of data. Scienti ...
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