Plant Stress Measurement
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Plant Stress Measurement
Plant stress measurement is the quantification of environmental effects on plant health. When plants are subjected to less than ideal growing conditions, they are considered to be under stress. Stress factors can affect growth, survival and crop yields. Plant stress research looks at the response of plants to limitations and excesses of the main abiotic factors ( light, temperature, water and nutrients), and of other stress factors that are important in particular situations (e.g. pests, pathogens, or pollutants). Plant stress measurement usually focuses on taking measurements from living plants. It can involve visual assessments of plant vitality, however, more recently the focus has moved to the use of instruments and protocols that reveal the response of particular processes within the plant (especially, photosynthesis, plant cell signalling and plant secondary metabolism) *Determining the optimal conditions for plant growth, e.g. optimising water use in an agricultural system * ...
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Quantification (science)
In mathematics and empirical science, quantification (or quantitation) is the act of counting and measuring that maps human sense observations and experiences into quantity, quantities. Quantification in this sense is fundamental to the scientific method. Natural science Some measure of the undisputed general importance of quantification in the natural sciences can be gleaned from the following comments: * "these are mere facts, but they are quantitative facts and the basis of science." * It seems to be held as universally true that "the foundation of quantification is measurement." * There is little doubt that "quantification provided a basis for the objectivity of science." * In ancient times, "musicians and artists ... rejected quantification, but merchants, by definition, quantified their affairs, in order to survive, made them visible on parchment and paper." * Any reasonable "comparison between Aristotle and Galileo shows clearly that there can be no unique lawfulness discov ...
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Chlorophyll Fluorescence
Chlorophyll fluorescence is light re-emitted by chlorophyll molecules during return from excited to non-excited states. It is used as an indicator of photosynthetic energy conversion in plants, algae and bacteria. Excited chlorophyll dissipates the absorbed light energy by driving photosynthesis (photochemical energy conversion), as heat in non-photochemical quenching or by emission as fluorescence radiation. As these processes are complementary processes, the analysis of chlorophyll fluorescence is an important tool in plant research with a wide spectrum of applications. The Kautsky effect Upon illumination of a dark-adapted leaf, there is a rapid rise in fluorescence from Photosystem II (PSII), followed by a slow decline. First observed by ''Kautsky et al., 1932'', this is called the Kautsky Effect. This variable rise in chlorophyll fluorescence rise is due to photosystem II. Fluorescence from photosystem I is not variable, but constant. The increase in fluorescence is du ...
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Breeding For Heat Stress Tolerance
Plant breeding is process of development of new cultivars. Plant breeding involves development of varieties for different environmental conditions – some of them are not favorable. Among them, heat stress is one of such factor that reduces the production and quality significantly. So breeding against heat is a very important criterion for breeding for current as well as future environments produced by global climate change (e.g. global warming). Breeding for heat stress tolerance in plants Heat stress due to increased temperature is a very important problem globally. Occasional or prolonged high temperatures cause different morpho-anatomical, physiological and biochemical changes in plants. The ultimate effect is on plant growth as well as development and reduced yield and quality. Breeding for heat stress tolerance can be mitigated by breeding plant varieties that have improved levels of thermo-tolerance using different conventional or advanced genetic tools. Marker assiste ...
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Breeding For Drought Stress Tolerance
Breeding for drought resistance is the process of breeding plants with the goal of reducing the impact of dehydration on plant growth. Dehydration stress Crop plants In nature or crop fields, water is often the most limiting factor for plant growth. If plants do not receive adequate rainfall or irrigation, the resulting dehydration stress can reduce growth more than all other environmental stresses combined. Drought can be defined as the absence of rainfall or irrigation for a period of time sufficient to deplete soil moisture and cause dehydration in plant tissues. Dehydration stress results when water loss from the plant exceeds the ability of the plant's roots to absorb water and when the plant's water content is reduced enough to interfere with normal plant processes. Global phenomenon About 15 million km2 of the land surface is covered by crop-land, and about 16% of this area is equipped for irrigation (Siebert ''et al.'' 2005). Thus, in many parts of the world, includi ...
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Adaptation To Global Warming
Climate change adaptation is the process of adjusting to current or expected effects of climate change.IPCC, 2022Annex II: Glossary öller, V., R. van Diemen, J.B.R. Matthews, C. Méndez, S. Semenov, J.S. Fuglestvedt, A. Reisinger (eds.) InClimate Change 2022: Impacts, Adaptation and Vulnerability. Contribution of Working Group II to the Sixth Assessment Report of the Intergovernmental Panel on Climate Change .-O. Pörtner, D.C. Roberts, M. Tignor, E.S. Poloczanska, K. Mintenbeck, A. Alegría, M. Craig, S. Langsdorf, S. Löschke, V. Möller, A. Okem, B. Rama (eds.) Cambridge University Press, Cambridge, UK and New York, NY, USA, pp. 2897–2930, doi:10.1017/9781009325844.029. For humans, adaptation aims to moderate or avoid harm, and exploit opportunities; for natural systems, humans may intervene to help adjustment. Adaptation actions can be either incremental (actions where the central aim is to maintain the essence and integrity of a system) or transformative (actions that chang ...
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Climate Change Mitigation
Climate change mitigation is action to limit climate change by reducing Greenhouse gas emissions, emissions of greenhouse gases or Carbon sink, removing those gases from the atmosphere. The recent rise in global average temperature is mostly caused by emissions from fossil fuels burning (coal, oil, and natural gas). Mitigation can reduce emissions by energy transition, transitioning to sustainable energy sources, energy conservation, conserving energy, and Efficient energy use, increasing efficiency. In addition, can be carbon dioxide removal, removed from the atmosphere by carbon sink, enlarging forests, Wetland restoration, restoring wetlands and using other natural and technical processes, which are grouped together under the term of carbon sequestration. Solar energy and wind power have the highest climate change mitigation potential at lowest cost compared to a range of other options. Variable availability of sunshine and wind is addressed by energy storage and improved elec ...
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Integrating Sphere
An integrating sphere (also known as an Ulbricht sphere) is an optical component consisting of a hollow spherical cavity with its interior covered with a diffuse reflection, diffuse white reflective coating, with small holes for entrance and exit ports. Its relevant property is a uniform scattering or diffusing effect. Light rays incident on any point on the inner surface are, by multiple scattering reflections, distributed equally to all other points. The effects of the original direction of light are minimized. An integrating sphere may be thought of as a diffuser (optics), diffuser which preserves power but destroys spatial information. It is typically used with some light source and a detector for optical power measurement. A similar device is the focusing or Coblentz sphere, which differs in that it has a mirror-like (specular) inner surface rather than a diffuse inner surface. In 1892, W. E. Sumpner published an expression for the throughput of a spherical enclosure with dif ...
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Photosystem I
Photosystem I (PSI, or plastocyanin–ferredoxin oxidoreductase) is one of two photosystems in the photosynthetic light reactions of algae, plants, and cyanobacteria. Photosystem I is an integral membrane protein complex that uses light energy to catalyze the transfer of electrons across the thylakoid membrane from plastocyanin to ferredoxin. Ultimately, the electrons that are transferred by Photosystem I are used to produce the moderate-energy hydrogen carrier NADPH. The photon energy absorbed by Photosystem I also produces a proton-motive force that is used to generate ATP. PSI is composed of more than 110 cofactors, significantly more than Photosystem II. History This photosystem is known as PSI because it was discovered before Photosystem II, although future experiments showed that Photosystem II is actually the first enzyme of the photosynthetic electron transport chain. Aspects of PSI were discovered in the 1950s, but the significance of these discoverie ...
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Electron Transport Chain
An electron transport chain (ETC) is a series of protein complexes and other molecules that transfer electrons from electron donors to electron acceptors via redox reactions (both reduction and oxidation occurring simultaneously) and couples this electron transfer with the transfer of protons (H+ ions) across a membrane. The electrons that transferred from NADH and FADH2 to the ETC involves 4 multi-subunit large enzymes complexes and 2 mobile electron carriers. Many of the enzymes in the electron transport chain are membrane-bound. The flow of electrons through the electron transport chain is an exergonic process. The energy from the redox reactions creates an electrochemical proton gradient that drives the synthesis of adenosine triphosphate (ATP). In aerobic respiration, the flow of electrons terminates with molecular oxygen as the final electron acceptor. In anaerobic respiration, other electron acceptors are used, such as sulfate. In an electron transport chain, the redo ...
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Measuring Fluorescence
Measurement is the quantification of attributes of an object or event, which can be used to compare with other objects or events. In other words, measurement is a process of determining how large or small a physical quantity is as compared to a basic reference quantity of the same kind. The scope and application of measurement are dependent on the context and discipline. In natural sciences and engineering, measurements do not apply to nominal properties of objects or events, which is consistent with the guidelines of the ''International vocabulary of metrology'' published by the International Bureau of Weights and Measures. However, in other fields such as statistics as well as the social and behavioural sciences, measurements can have multiple levels, which would include nominal, ordinal, interval and ratio scales. Measurement is a cornerstone of trade, science, technology and quantitative research in many disciplines. Historically, many measurement systems existed for t ...
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Photosystem II
Photosystem II (or water-plastoquinone oxidoreductase) is the first protein complex in the light-dependent reactions of oxygenic photosynthesis. It is located in the thylakoid membrane of plants, algae, and cyanobacteria. Within the photosystem, enzymes capture photons of light to energize electrons that are then transferred through a variety of coenzymes and cofactors to reduce plastoquinone to plastoquinol. The energized electrons are replaced by oxidizing water to form hydrogen ions and molecular oxygen. By replenishing lost electrons with electrons from the splitting of water, photosystem II provides the electrons for all of photosynthesis to occur. The hydrogen ions (protons) generated by the oxidation of water help to create a proton gradient that is used by ATP synthase to generate ATP. The energized electrons transferred to plastoquinone are ultimately used to reduce to NADPH or are used in non-cyclic electron flow. DCMU is a chemical often used in laboratory sett ...
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Transpiration
Transpiration is the process of water movement through a plant and its evaporation from aerial parts, such as leaves, stems and flowers. Water is necessary for plants but only a small amount of water taken up by the roots is used for growth and metabolism. The remaining 97–99.5% is lost by transpiration and guttation. Leaf surfaces are dotted with pores called stomata (singular "stoma"), and in most plants they are more numerous on the undersides of the foliage. The stomata are bordered by guard cells and their stomatal accessory cells (together known as stomatal complex) that open and close the pore. Transpiration occurs through the stomatal apertures, and can be thought of as a necessary "cost" associated with the opening of the stomata to allow the diffusion of carbon dioxide gas from the air for photosynthesis. Transpiration also cools plants, changes osmotic pressure of cells, and enables mass flow of mineral nutrients and water from roots to shoots. Two major factors i ...
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