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Prototheca Zopfii
''Prototheca zopfii'' is aerobic, unicellular, yeast-like, achlorophyllic (without chlorophyll) microalga. Distribution ''Prototheca zopfii'' is opportunistic, environmental pathogen and ubiquitous in nature. This alga is mainly associated with wet areas and places with high organic contents. It can be found in tanks, well water, teat-dip containers, and milking machines. Genome The genome of this organism's mitochondrion and plastid were first sequenced in 2018.Severgnini M, Lazzari B, Capra E, Chessa S, Luini M, Bordoni R, Castiglioni B, Ricchi M, Cremonesi P (2018) Genome sequencing of Prototheca zopfii genotypes 1 and 2 provides evidence of a severe reduction in organellar genomes. Sci Rep 8(1):14637. doi: 10.1038/s41598-018-32992-0. Reproduction ''Prototheca zopfii'' reproduce asexually by endosporulation. Culture media Sabouraud agar is used as a cultural medium.Antimicrobial susceptibility of'' Prototheca zopfii'' isolated from bovine mastitis., Władysław Waw ...
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Pathogen
In biology, a pathogen ( el, πάθος, "suffering", "passion" and , "producer of") in the oldest and broadest sense, is any organism or agent that can produce disease. A pathogen may also be referred to as an infectious agent, or simply a germ. The term ''pathogen'' came into use in the 1880s. Typically, the term ''pathogen'' is used to describe an ''infectious'' microorganism or agent, such as a virus, bacterium, protozoan, prion, viroid, or fungus. Small animals, such as helminths and insects, can also cause or transmit disease. However, these animals are usually referred to as parasites rather than pathogens. The scientific study of microscopic organisms, including microscopic pathogenic organisms, is called microbiology, while parasitology refers to the scientific study of parasites and the organisms that host them. There are several pathways through which pathogens can invade a host. The principal pathways have different episodic time frames, but soil has the longest ...
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Organic Matter
Organic matter, organic material, or natural organic matter refers to the large source of carbon-based compounds found within natural and engineered, terrestrial, and aquatic environments. It is matter composed of organic compounds that have come from the feces and remains of organisms such as plants and animals. Organic molecules can also be made by chemical reactions that do not involve life. Basic structures are created from cellulose, tannin, cutin, and lignin, along with other various proteins, lipids, and carbohydrates. Organic matter is very important in the movement of nutrients in the environment and plays a role in water retention on the surface of the planet. Formation Living organisms are composed of organic compounds. In life, they secrete or excrete organic material into their environment, shed body parts such as leaves and roots and after organisms die, their bodies are broken down by bacterial and fungal action. Larger molecules of organic matter can be formed ...
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Mitochondrion
A mitochondrion (; ) is an organelle found in the cells of most Eukaryotes, such as animals, plants and fungi. Mitochondria have a double membrane structure and use aerobic respiration to generate adenosine triphosphate (ATP), which is used throughout the cell as a source of chemical energy. They were discovered by Albert von Kölliker in 1857 in the voluntary muscles of insects. The term ''mitochondrion'' was coined by Carl Benda in 1898. The mitochondrion is popularly nicknamed the "powerhouse of the cell", a phrase coined by Philip Siekevitz in a 1957 article of the same name. Some cells in some multicellular organisms lack mitochondria (for example, mature mammalian red blood cells). A large number of unicellular organisms, such as microsporidia, parabasalids and diplomonads, have reduced or transformed their mitochondria into other structures. One eukaryote, ''Monocercomonoides'', is known to have completely lost its mitochondria, and one multicellular organism, '' ...
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Plastid
The plastid (Greek: πλαστός; plastós: formed, molded – plural plastids) is a membrane-bound organelle found in the Cell (biology), cells of plants, algae, and some other eukaryotic organisms. They are considered to be intracellular endosymbiotic cyanobacteria. Examples include chloroplasts (used for photosynthesis), chromoplasts (used for pigment synthesis and storage), and leucoplasts (non-pigmented plastids that can sometimes differentiate). The event which led to permanent endosymbiosis in the Archaeplastida clade (of Embryophyte, land plants, red algae, and green algae) probably occurred with a cyanobiont (a symbiotic cyanobacteria) related to the genus ''Gloeomargarita lithophora, Gloeomargarita'', around 1.5 billion years ago. A later primary endosymbiosis event occurred in photosynthetic ''Paulinella'' amoeboids about 90–140 million years ago. This plastid belongs to the "PS-clade" (of the cyanobacteria genera ''Prochlorococcus'' and ''Synechococcus''). Chloroplas ...
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Sabouraud Agar
Sabouraud agar or Sabouraud dextrose agar (SDA) is a type of agar growth medium containing peptones. It is used to cultivate dermatophytes and other types of fungi, and can also grow filamentous bacteria such as '' Nocardia''. It has utility for research and clinical care. It was created by, and is named after, Raymond Sabouraud in 1892. In 1977 the formulation was adjusted by Chester W. Emmons when the pH level was brought closer to the neutral range and the dextrose concentration lowered to support the growth of other microorganisms. The acidic pH (5.6) of traditional Sabouraud agar inhibits bacterial growth. Typical composition Sabouraud agar is commercially available and typically contains:University of Sydney''Recipes'' * 40 g/L dextrose * 10 g/L peptone * 20 g/L agar * pH 5.6 Medical Use Clinical laboratories can use this growth medium to diagnose and further speciate fungal infections, allowing medical professionals to provide appropriate treatment with antifunga ...
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Polymerase Chain Reaction
The polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies (complete or partial) of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it (or a part of it) to a large enough amount to study in detail. PCR was invented in 1983 by the American biochemist Kary Mullis at Cetus Corporation; Mullis and biochemist Michael Smith (chemist), Michael Smith, who had developed other essential ways of manipulating DNA, were jointly awarded the Nobel Prize in Chemistry in 1993. PCR is fundamental to many of the procedures used in genetic testing and research, including analysis of Ancient DNA, ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory research for a broad variety of applications ...
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Mastitis
Mastitis is inflammation of the breast or udder, usually associated with breastfeeding. Symptoms typically include local pain and redness. There is often an associated fever and general soreness. Onset is typically fairly rapid and usually occurs within the first few months of delivery. Complications can include abscess formation. Risk factors include poor latch, cracked nipples, use of a breast pump, and weaning. The bacteria most commonly involved are '' Staphylococcus'' and '' Streptococci''. Diagnosis is typically based on symptoms. Ultrasound may be useful for detecting a potential abscess. Prevention is by proper breastfeeding techniques. When infection is present, antibiotics such as cephalexin may be recommended. Breastfeeding should typically be continued, as emptying the breast is important for healing. Tentative evidence supports benefits from probiotics. About 10% of breastfeeding women are affected. Types When it occurs in breastfeeding mothers, it is known as puer ...
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