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Potato Leafroll Virus
Potato leafroll virus (PLRV) is a member of the genus ''Polerovirus'' and family ''Solemoviridae''. The phloem limited positive sense RNA virus infects potatoes and other members of the family Solanaceae. PLRV was first described by Quanjer ''et al.'' in 1916. PLRV is transmitted by aphids, primarily the green peach aphid, ''Myzus persicae''. PLRV is one of the most important potato viruses worldwide but particularly devastating in countries with limited resources and management. It can be responsible for individual plant yield losses of over 50%. One estimate suggests that PLRV is responsible for an annual global yield loss of 20 million tons. Symptoms include chlorosis, necrosis and leaf curling. Hosts and symptoms PLRV infects members of the family Solanaceae. The most economically important host is the Potato, ''Solanum tuberosum'' spp. In potato, symptoms of primary infection, infection in the growing season, occurs in the youngest leaves. Leaf margins become necrotic, ...
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Polerovirus
''Polerovirus'' is a genus of viruses, in the family '' Solemoviridae''. Plants serve as natural hosts. There are 26 species in this genus. Diseases associated with this genus include: PLRV causes prominent rolling of the leaves of potato and a stiff upright habit of the plants; necrosis of the phloem and accumulation of carbohydrates in the leaves. Taxonomy The following species are recognized: * '' Beet chlorosis virus'' * '' Beet mild yellowing virus'' * ''Beet western yellows virus'' * ''Carrot red leaf virus'' * '' Cereal yellow dwarf virus RPS'' * '' Cereal yellow dwarf virus RPV'' * '' Chickpea chlorotic stunt virus'' * '' Cotton leafroll dwarf virus'' * '' Cucurbit aphid-borne yellows virus'' * '' Faba bean polerovirus 1'' * '' Maize yellow dwarf virus RMV'' * '' Maize yellow mosaic virus'' * '' Melon aphid-borne yellows virus'' * '' Pepo aphid-borne mosaic virus'' * '' Pepper vein yellows virus 1'' * ''Pepper vein yellows virus 2'' * '' Pepper vein yellows virus 3'' * ...
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Phloem
Phloem (, ) is the living biological tissue, tissue in vascular plants that transports the soluble organic compounds made during photosynthesis and known as ''photosynthates'', in particular the sugar sucrose, to the rest of the plant. This transport process is called translocation. In trees, the phloem is the innermost layer of the bark (botany), bark, hence the name, derived from the Ancient Greek word (''phloiós''), meaning "bark". The term was introduced by Carl Nägeli in 1858. Structure Phloem tissue consists of conducting Cell (biology), cells, generally called sieve elements, Ground tissue#Parenchyma, parenchyma cells, including both specialized companion cells or albuminous cells and unspecialized cells and supportive cells, such as fibres and sclereids. Conducting cells (sieve elements) Sieve elements are the type of cell that are responsible for transporting sugars throughout the plant. At maturity they lack a Cell nucleus, nucleus and have very few organelles, ...
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Viral Diseases Of Potato
Viral diseases of potato are a group of diseases caused by different types of viruses that affect potato crops worldwide and, although they do not affect human or animal health since they are viruses that only infect vegetables, they are a source of great economic losses annually. About 28 viruses have been reported infecting potato crops. However, potato virus X ( PVX), potato virus Y ( PVY), and potato leafroll virus ( PLRV) are the most important viruses worldwide. Some others are of economic importance only in some regions. Such is the case of potato virus M (PVM) in some Asian and European countries. An additional problem is the co-infection of two or more viruses on the same plants. In fact, the joint occurrence of PVX and PVY, or either or both of them with PLRV or Potato virus A ( PVA), produces much more severe symptoms than separate infection of each. The magnitude of economic losses associated with this synergistic effect depends on the types of viruses that are intera ...
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Potato Spindle Tuber Viroid
The potato spindle tuber viroid (PSTVd) was the first viroid to be identified.Discovery of Viroids
PSTVd is a small, single stranded molecule closely related to the chrysanthemum stunt viroid. Present within the viroidal RNA is the common to its genus. The natural hosts are

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Viroid
Viroids are small single-stranded, circular RNAs that are infectious pathogens. Unlike viruses, they have no protein coating. All known viroids are inhabitants of angiosperms (flowering plants), and most cause diseases, whose respective economic importance to humans varies widely. The first discoveries of viroids in the 1970s triggered the historically third major extension of the biosphere—to include smaller lifelike entities —after the discoveries in 1675 by Antonie van Leeuwenhoek (of the "subvisible" microorganisms) and in 1892–1898 by Dmitri Iosifovich Ivanovsky and Martinus Beijerinck (of the "submicroscopic" viruses). The unique properties of viroids have been recognized by the International Committee on Taxonomy of Viruses, in creating a new order of subviral agents. The first recognized viroid, the pathogenic agent of the potato spindle tuber disease, was discovered, initially molecularly characterized, and named by Theodor Otto Diener, plant pathologist a ...
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ELISA
The enzyme-linked immunosorbent assay (ELISA) (, ) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence of a ligand (commonly a protein) in a liquid sample using antibodies directed against the protein to be measured. ELISA has been used as a diagnostic tool in medicine, plant pathology, and biotechnology, as well as a quality control check in various industries. In the most simple form of an ELISA, antigens from the sample to be tested are attached to a surface. Then, a matching antibody is applied over the surface so it can bind the antigen. This antibody is linked to an enzyme and then any unbound antibodies are removed. In the final step, a substance containing the enzyme's substrate is added. If there was binding, the subsequent reaction produces a detectable signal, most commonly a color change. Performing an ELISA involves at least ...
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Endosulfan
Endosulfan is an off-patent organochlorine insecticide and acaricide that is being phased out globally. It became a highly controversial agrichemical due to its acute toxicity, potential for bioaccumulation, and role as an endocrine disruptor. Because of its threats to human health and the environment, a global ban on the manufacture and use of endosulfan was negotiated under the Stockholm Convention in April 2011. The ban has taken effect in mid-2012, with certain uses exempted for five additional years. More than 80 countries, including the European Union, Australia, New Zealand, several West African nations, the United States,Cone, MarlaEPA Bans Pesticide Found on Cucumbers, Zucchini, Green Beans and Other Vegetables.''The Daily Green.'' June 10, 2010. Brazil, and Canada had already banned it or announced phase-outs by the time the Stockholm Convention ban was agreed upon. It is still used extensively in India and China despite laws against its use. It is also used in a few o ...
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Methamidophos
Methamidophos, trade name "Monitor," is an organophosphate insecticide. Crops grown with the use of methamidophos include potatoes and some Latin American rice. Many nations have used methamidophos on crops, including developed nations such as Spain, United States, Japan, and Australia. Due to its toxicity, the use of pesticides that contain methamidophos is currently being phased out in Brazil. In 2009, all uses in the United States were voluntarily canceled. Toxicity rates of 21 and 16 mg/kg for male and female rats, respectively. 10–30 mg/kg in rabbits, and dermal LD50 of 50 mg/kg in rats. It is rapidly absorbed through the stomach, lungs, and skin in humans, and eliminated primarily through urine. It is a cholinesterase inhibitor. Breakdown in soil is 6.1 days in sand, 309 days in water at pH 5.0, 27 days at pH 7.0, and 3 days at pH 9.0. Sunlight accelerates breakdown. It is uptaken through roots and leaves of plants. It is classified as a WHO Toxicit ...
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Imidacloprid
Imidacloprid is a systemic insecticide belonging to a class of chemicals called the neonicotinoids which act on the central nervous system of insects. The chemical works by interfering with the transmission of stimuli in the insect nervous system. Specifically, it causes a blockage of the nicotinergic neuronal pathway. By blocking nicotinic acetylcholine receptors, imidacloprid prevents acetylcholine from transmitting impulses between nerves, resulting in the insect's paralysis and eventual death. It is effective on contact and via stomach action. Because imidacloprid binds much more strongly to insect neuron receptors than to mammal neuron receptors, this insecticide is more toxic to insects than to mammals. From 1999 through , imidacloprid was the most widely used insecticide in the world. Although it is now off patent, the primary manufacturer of this chemical is Bayer CropScience (part of Bayer AG). It is sold under many names for many uses; it can be applied by soil injection ...
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Insecticides
Insecticides are substances used to kill insects. They include ovicides and larvicides used against insect eggs and larvae, respectively. Insecticides are used in agriculture, medicine, industry and by consumers. Insecticides are claimed to be a major factor behind the increase in the 20th-century's agricultural productivity. Nearly all insecticides have the potential to significantly alter ecosystems; many are toxic to humans and/or animals; some become concentrated as they spread along the food chain. Insecticides can be classified into two major groups: systemic insecticides, which have residual or long term activity; and contact insecticides, which have no residual activity. The mode of action describes how the pesticide kills or inactivates a pest. It provides another way of classifying insecticides. Mode of action can be important in understanding whether an insecticide will be toxic to unrelated species, such as fish, birds and mammals. Insecticides may be repellent ...
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Desiccation
Desiccation () is the state of extreme dryness, or the process of extreme drying. A desiccant is a hygroscopic (attracts and holds water) substance that induces or sustains such a state in its local vicinity in a moderately sealed container. Industry Desiccation is widely employed in the oil and gas industry. These materials are obtained in a hydrated state, but the water content leads to corrosion or is incompatible with downstream processing. Removal of water is achieved by cryogenic condensation, absorption into glycols, and absorption onto desiccants such as silica gel. Laboratory A desiccator is a heavy glass or plastic container, now somewhat antiquated, used in practical chemistry for drying or keeping small amounts of materials very dry. The material is placed on a shelf, and a drying agent or ''desiccant'', such as dry silica gel or anhydrous sodium hydroxide, is placed below the shelf. Often some sort of humidity indicator is included in the desiccator to show, ...
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Instar
An instar (, from the Latin '' īnstar'', "form", "likeness") is a developmental stage of arthropods, such as insects, between each moult (''ecdysis''), until sexual maturity is reached. Arthropods must shed the exoskeleton in order to grow or assume a new form. Differences between instars can often be seen in altered body proportions, colors, patterns, changes in the number of body segments or head width. After shedding their exoskeleton (moulting), the juvenile arthropods continue in their life cycle until they either pupate or moult again. The instar period of growth is fixed; however, in some insects, like the salvinia stem-borer moth, the number of instars depends on early larval nutrition. Some arthropods can continue to moult after sexual maturity, but the stages between these subsequent moults are generally not called instars. For most insect species, an ''instar'' is the developmental stage of the larval forms of holometabolous (complete metamorphism) or nymphal forms o ...
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