Phlorotannin
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Phlorotannin
Phlorotannins are a type of tannins found in brown algae such as kelps and rockweeds or sargassacean species, and in a lower amount also in some red algae. Contrary to hydrolysable or condensed tannins, these compounds are oligomers of phloroglucinol (polyphloroglucinols). As they are called tannins, they have the ability to precipitate proteins. It has been noticed that some phlorotannins have the ability to oxidize and form covalent bonds with some proteins. In contrast, under similar experimental conditions three types of terrestrial tannins (procyanidins, profisetinidins, and gallotannins) apparently did not form covalent complexes with proteins. These phenolic compounds are integral structural components of cell walls in brown algae, but they also seem to play many other secondary ecological roles such as protection from UV radiation and defense against grazing. Biosynthesis and localization Most of the phlorotannins' biosynthesis is still unknown, but it appears they ar ...
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Tetrafucol A
Tetrafucol A is a fucol-type phlorotannin found in the brown algae ''Ascophyllum nodosum'', ''Analipus japonicus'' and ''Scytothamnus australis ''Scytothamnus australis'' is a brown alga species in the genus ''Scytothamnus'' found in New Zealand. It is a sulphated polysaccharide and the type species in the genus. The species contains the phlorotannins trifucol, tetrafucol A, tetrafuco ...''. In ''A. japonicus'', 5'-bromo- and 5'-chlorotetrafucol-A can also be detected. References Phlorotannins Natural phenol tetramers {{aromatic-stub ...
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Ascophyllum Nodosum
''Ascophyllum nodosum'' is a large, common cold water seaweed or brown alga (Phaeophyceae) in the family Fucaceae, being the only species in the genus ''Ascophyllum''. It is a seaweed that only grows in the northern Atlantic Ocean, also known in localities as feamainn bhuí, rockweed, Norwegian kelp, knotted kelp, knotted wrack or egg wrack. It is common on the north-western coast of Europe (from the White Sea to Portugal) including east Greenland and the north-eastern coast of North America, its range further south of these latitudes being limited by warmer ocean waters. Description ''Ascophyllum nodosum'' has long tough and leathery fronds,Bunker, F.StP., Maggs, C.A., Brodie, J.A. and Bunker, J.A. 2017. ''Seaweeds of Britain and Ireland.'' Second Edition. Wild Nature Press, Plymouth, UK. irregularly dichotomously branched fronds with large, egg-shaped air bladders set in series at regular intervals along the fronds and not stalked. The fronds can reach 2 m in length and a ...
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Natural Phenol
In biochemistry, naturally occurring phenols are natural products containing at least one phenol functional group. Phenolic compounds are produced by plants and microorganisms. Organisms sometimes synthesize phenolic compounds in response to ecological pressures such as pathogen and insect attack, UV radiation and wounding. As they are present in food consumed in human diets and in plants used in traditional medicine of several cultures, their role in human health and disease is a subject of research. Some phenols are germicidal and are used in formulating disinfectants. Classification Various classification schemes can be applied. A commonly used scheme is based on the number of carbons and was devised by Jeffrey Harborne and Simmonds in 1964 and published in 1980: C6-C7-C6 Diarylheptanoids are not included in this Harborne classification. They can also be classified on the basis of their number of phenol groups. They can therefore be called ''simple phenols'' or ...
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Phloroglucinol
Phloroglucinol is an organic compound with the formula C6H3(OH)3. It is a colorless solid. It is used in the synthesis of pharmaceuticals and explosives. Phloroglucinol is one of three isomeric benzenetriols. The other two isomers are hydroxyquinol (1,2,4-benzenetriol) and pyrogallol (1,2,3-benzenetriol). Phloroglucinol, and its benzenetriol isomers, are still defined as "phenols" according to the IUPAC official nomenclature rules of chemical compounds. Many such monophenolics are often termed "polyphenols" by the cosmetic and parapharmaceutical industries, which does not match the scientifically accepted definition. Synthesis and occurrence In 1855, phloroglucinol was first prepared from phloretin by the Austrian chemist Heinrich Hlasiwetz (1825–1875). A modern synthesis of involves hydrolysis of benzene-1,3,5-triamine and its derivatives. Representative is the following route from trinitrobenzene. : The synthesis is noteworthy because ordinary aniline derivatives are unre ...
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Tannin
Tannins (or tannoids) are a class of astringent, polyphenolic biomolecules that bind to and precipitate proteins and various other organic compounds including amino acids and alkaloids. The term ''tannin'' (from Anglo-Norman ''tanner'', from Medieval Latin ''tannāre'', from ''tannum'', oak bark) refers to the use of oak and other bark in tanning animal hides into leather. By extension, the term ''tannin'' is widely applied to any large polyphenolic compound containing sufficient hydroxyls and other suitable groups (such as carboxyls) to form strong complexes with various macromolecules. The tannin compounds are widely distributed in many species of plants, where they play a role in protection from predation (acting as pesticides) and might help in regulating plant growth. The astringency from the tannins is what causes the dry and puckery feeling in the mouth following the consumption of unripened fruit, red wine or tea. Likewise, the destruction or modification of t ...
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Eckol
Eckol is a phlorotannin isolated from brown algae in the family Lessoniaceae such as species in the genus ''Ecklonia'' such as '' E. cava'' or '' E. kurome'' or in the genus '' Eisenia'' such as ''Eisenia bicyclis''. The molecule possesses a dibenzo-''p''-dioxin skeleton and a phloroglucinol component. The molecule can also be viewed as a phloroglucinol trimer. It exhibits an antiplasmin inhibitory effect and a radioprotective effect in a mouse model. It also exhibits an ''in vitro'' cytoprotective effect against oxidative stress in Chinese hamster lung fibroblasts. It also shows antithrombotic and profibrinolytic Fibrinolysis is a process that prevents blood clots from growing and becoming problematic. Primary fibrinolysis is a normal body process, while secondary fibrinolysis is the breakdown of clots due to a medicine, a medical disorder, or some other ... activities. References {{phlorotannin Phlorotannins Dibenzodioxins ...
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Vanillin–HCl Staining
Vanillin–HCl staining (10% vanillin and 90% of a mixture of ethanol and HCl, giving an orange color) can be used to visualize the localisation of tannins in cells. The localization of phlorotannins can be investigated by light microscopy after vanillin–HCl staining.Cytological studies on physodes in the vegetative cells of Cystoseira stricter Sauvagea (Phaeophyta, Fucales). Pellegrini L, J. Cell Sci., 1980, volume 41, pages 209–231 The phlorotannins can be seen this way in physodes in brown algae. The vanillin-HCl method can be used to estimate the proanthocyanidins content in plant cells, resulting in a red color of the test in the presence of catechin Catechin is a flavan-3-ol, a type of secondary metabolite providing antioxidant roles in plants. It belongs to the subgroup of polyphenols called flavonoids. The name of the catechin chemical family derives from ''catechu'', which is the tannic ... or proanthocyanidins. References Staining {{cell-biology-stub ...
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Prussian Blue Assay
Prussian blue (also known as Berlin blue, Brandenburg blue or, in painting, Parisian or Paris blue) is a dark blue pigment produced by oxidation of ferrous ferrocyanide salts. It has the chemical formula Fe CN)">Cyanide.html" ;"title="e(Cyanide">CN) Turnbull's blue is chemically identical, but is made from different reagents, and its slightly different color stems from different impurities and particle sizes. Prussian blue was the first modern synthetic pigment. It is prepared as a very fine colloidal dispersion, because the compound is not soluble in water. It contains variable amounts of other ions and its appearance depends sensitively on the size of the colloidal particles. The pigment is used in paints, and it is the traditional "blue" in blueprints, and became prominent in 19th-century () Japanese woodblock prints. In medicine, orally administered Prussian blue is used as an antidote for certain kinds of heavy metal poisoning, e.g., by thallium(I) and radioactive isot ...
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Ascorbic Acid
Vitamin C (also known as ascorbic acid and ascorbate) is a water-soluble vitamin found in citrus and other fruits and vegetables, also sold as a dietary supplement and as a topical 'serum' ingredient to treat melasma (dark pigment spots) and wrinkles on the face. It is used to prevent and treat scurvy. Vitamin C is an essential nutrient involved in the repair of tissue, the formation of collagen, and the enzymatic production of certain neurotransmitters. It is required for the functioning of several enzymes and is important for immune system function. It also functions as an antioxidant. Most animals are able to synthesize their own vitamin C. However, apes (including humans) and monkeys (but not all primates), most bats, some rodents, and certain other animals must acquire it from dietary sources. There is some evidence that regular use of supplements may reduce the duration of the common cold, but it does not appear to prevent infection. It is unclear whether supp ...
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Glutaraldehyde
Glutaraldehyde is an organic compound with the formula . The molecule consists of a five carbon chain doubly terminated with formyl (CHO) groups. It is usually used as a solution in water, and such solutions exists as a collection of hydrates, cyclic derivatives, and condensation products, several of which interconvert. Because the molecule has two carbonyl group is reactive to primary amine groups (even as its hydrates), it can function as a crosslinking agent for any substance with primary amine groups and develop imine connected links. Crosslinking rigidifies and deactivates many biological functions, so in this way, glutaraldehyde solutions are used as biocides and as fixative. It is sold under the brandname Cidex and Glutaral. As a disinfectant, it is used to sterilize surgical instruments. Uses Biochemistry Glutaraldehyde is used in biochemistry applications as an amine-reactive homobifunctional crosslinker and fixative. It kills cells quickly by crosslinking their ...
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