Ovorubin
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Ovorubin
Ovorubin (PcOvo or PcPV1) is the most abundant perivitellin (>60 % total protein) of the perivitelline fluid from ''Pomacea canaliculata'' snail eggs. This glyco-lipo-caroteno protein complex is a approx. 300 kDa multimer of a combination of multiple copies of six different ~30 kDa subunits. Together with the other perivitellins from ''Pomacea canaliculata'' eggs, ovorubin serves a nutrient source for developing embryos, notably to the intermediate and late stages. Moreover, after hatching, the protein is still detected in the lumen of the digestive gland ready to be endocytosed, therefore, acting as a nutrient source for the newly hatched snail. Ovorubin contains carbohydrates and carotenoid pigments as main prosthetic groups, which are related to many physiological roles on ''Pomacea'' aerial egg-laying strategy. Given that carbohydrates tend to retain water, the high glycosylation of ovorubin (~17 % w/w) was proposed as an embryo defense against water loss. The carotenoid p ...
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Perivitellins
Perivitellins are egg proteins found in the perivitelline fluid of many gastropods. They are multifunctional complexes providing the developing embryo with nutrition, protection from the environment, and defense against predators. Despite the central role perivitellins play in reproduction and development, there is little information about their role in gastropod Molluscs. Most studies of perivitellins have been performed in eggs of Ampullaridae, a family of freshwater snails (Caenogastropoda), notably the ''Pomacea'' genus, mostly those of ''Pomacea canaliculata'', ''Pomacea scalaris'' and ''Pomacea maculata''. Synthesis Perivitellins are almost exclusively synthesized in the albumen gland (also known as albumen gland-capsule gland complex or uterine gland), an accessory gland from the female reproductive system of gastropods. This has been experimentally confirmed for the perivitellins ovorubin (PcOvo) and PcPV2, which were only found in the albumen gland with no extra-gland ...
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Pomacea Canaliculata
''Pomacea canaliculata'', commonly known as the golden apple snail or the channeled apple snail, is a species of large freshwater snail with gills and an operculum, an aquatic gastropod mollusc in the family Ampullariidae, the apple snails. South American in origin, this species is considered to be in the top 100 of the "World's Worst Invasive Alien Species". It is also ranked as the 40th worst alien species in Europe and the worst alien species of gastropod in Europe. Distribution The native distribution of ''P. canaliculata'' is basically tropical and subtropical, including Argentina, Bolivia, Paraguay, Uruguay, and Brazil. The southernmost record for the species is Paso de las Piedras reservoir, south of the Buenos Aires province, Argentina. Non-indigenous distribution This species also occurs in the United States, where the initial introductions were probably from aquarium release, aka "aquarium dumping". The non-indigenous distribution includes: Lake Wawasee in K ...
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Perivitellins
Perivitellins are egg proteins found in the perivitelline fluid of many gastropods. They are multifunctional complexes providing the developing embryo with nutrition, protection from the environment, and defense against predators. Despite the central role perivitellins play in reproduction and development, there is little information about their role in gastropod Molluscs. Most studies of perivitellins have been performed in eggs of Ampullaridae, a family of freshwater snails (Caenogastropoda), notably the ''Pomacea'' genus, mostly those of ''Pomacea canaliculata'', ''Pomacea scalaris'' and ''Pomacea maculata''. Synthesis Perivitellins are almost exclusively synthesized in the albumen gland (also known as albumen gland-capsule gland complex or uterine gland), an accessory gland from the female reproductive system of gastropods. This has been experimentally confirmed for the perivitellins ovorubin (PcOvo) and PcPV2, which were only found in the albumen gland with no extra-gland ...
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Protein
Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, responding to stimuli, providing structure to cells and organisms, and transporting molecules from one location to another. Proteins differ from one another primarily in their sequence of amino acids, which is dictated by the nucleotide sequence of their genes, and which usually results in protein folding into a specific 3D structure that determines its activity. A linear chain of amino acid residues is called a polypeptide. A protein contains at least one long polypeptide. Short polypeptides, containing less than 20–30 residues, are rarely considered to be proteins and are commonly called peptides. The individual amino acid residues are bonded together by peptide bonds and adjacent amino acid residues. The sequence of amino acid residue ...
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Perivitelline Fluid
The perivitelline fluid is an extracellular fluid found in the eggs of most gastropods and constitutes the main source of nutrition and defense for their embryos. It replaces the egg yolk of other animals, which in snail eggs is reduced to non-nutritive proteinaceous granules with putative enzymatic function. During embryonic development the perivitelline fluid is ingested macropinocytotically by the embryos and the resulting phagosomes fuse with β-granules containing hydrolytic enzymes, which digest the perivitelline fluid components. Origin The perivitelline fluid is synthesized by the albumen gland of female snails (also known as albumen gland-capsule gland complex or uterine gland), an accessory gland from the reproductive tract. Fertilized oocytes enter the albumen gland and, on their way out, are coated with the perivitelline fluid. The amount of perivitelline fluid per egg vary considerably among species. However, the amount of perivitelline fluid per egg is constant wit ...
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Endocytosis
Endocytosis is a cellular process in which substances are brought into the cell. The material to be internalized is surrounded by an area of cell membrane, which then buds off inside the cell to form a vesicle containing the ingested material. Endocytosis includes pinocytosis (cell drinking) and phagocytosis (cell eating). It is a form of active transport. History The term was proposed by De Duve in 1963. Phagocytosis was discovered by Élie Metchnikoff in 1882. Pathways Endocytosis pathways can be subdivided into four categories: namely, receptor-mediated endocytosis (also known as clathrin-mediated endocytosis), caveolae, pinocytosis, and phagocytosis Phagocytosis () is the process by which a cell uses its plasma membrane to engulf a large particle (≥ 0.5 μm), giving rise to an internal compartment called the phagosome. It is one type of endocytosis. A cell that performs phagocytosis is .... *Clathrin-mediated endocytosis is mediated by the production of smal ...
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Carbohydrate
In organic chemistry, a carbohydrate () is a biomolecule consisting of carbon (C), hydrogen (H) and oxygen (O) atoms, usually with a hydrogen–oxygen atom ratio of 2:1 (as in water) and thus with the empirical formula (where ''m'' may or may not be different from ''n''), which does not mean the H has covalent bonds with O (for example with , H has a covalent bond with C but not with O). However, not all carbohydrates conform to this precise stoichiometric definition (e.g., uronic acids, deoxy-sugars such as fucose), nor are all chemicals that do conform to this definition automatically classified as carbohydrates (e.g. formaldehyde and acetic acid). The term is most common in biochemistry, where it is a synonym of saccharide (), a group that includes sugars, starch, and cellulose. The saccharides are divided into four chemical groups: monosaccharides, disaccharides, oligosaccharides, and polysaccharides. Monosaccharides and disaccharides, the smallest (lower molecular wei ...
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Carotenoid
Carotenoids (), also called tetraterpenoids, are yellow, orange, and red organic compound, organic pigments that are produced by plants and algae, as well as several bacteria, and Fungus, fungi. Carotenoids give the characteristic color to pumpkins, carrots, parsnips, maize, corn, tomatoes, Domestic Canary, canaries, flamingos, salmon, lobster, shrimp, and daffodils. Carotenoids can be produced from Lipid, fats and other basic organic metabolic building blocks by all these organisms. It is also produced by Endosymbiont, endosymbiotic bacteria in Whitefly, whiteflies. Carotenoids from the diet are stored in the fatty tissues of animals, and exclusively Carnivore, carnivorous animals obtain the compounds from animal fat. In the human diet, Small intestine#Absorption, absorption of carotenoids is improved when consumed with fat in a meal. Cooking carotenoid-containing vegetables in oil and shredding the vegetable both increase carotenoid bioavailability. There are over 1,100 known c ...
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Prosthetic Group
A prosthetic group is the non-amino acid component that is part of the structure of the heteroproteins or conjugated proteins, being tightly linked to the apoprotein. Not to be confused with the cofactor that binds to the enzyme apoenzyme (either a holoprotein or heteroprotein) by non-covalent binding a non-protein (non-amino acid) This is a component of a conjugated protein that is required for the protein's biological activity. The prosthetic group may be organic (such as a vitamin, sugar, RNA, phosphate or lipid) or inorganic (such as a metal ion). Prosthetic groups are bound tightly to proteins and may even be attached through a covalent bond. They often play an important role in enzyme catalysis. A protein without its prosthetic group is called an apoprotein, while a protein combined with its prosthetic group is called a holoprotein. A non-covalently bound prosthetic group cannot generally be removed from the holoprotein without denaturating the protein. Thus, the term ...
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Pomacea
''Pomacea'' is a genus of freshwater snails with gills and an operculum, aquatic gastropod mollusks in the family Ampullariidae, the apple snails. The genus is native to the Americas; most species in this genus are restricted to South America. in the aquarium trade these snails are sometimes called ''Pomacea'' or incorrectly ''Ampullarius'', and in English as "olormystery snail" or "apple snail". Some species have been introduced outside their native range and are considered invasive because of their voracious appetite for plants. Because of this, imports involving this genus are restricted in some regions (including the United States) and are entirely banned in others (including the EU).Dawes, J. (14 January 2013). International Waters: EU Finally Bans Apple Snail Imports.'' Retrieved 4 June 2014 Species Species in the genus ''Pomacea'' include: subgenus ''Effusa'' Jousseaume, 1889 * ''Pomacea baeri'' (Dautzenberg, 1902) * ''Pomacea glauca'' (Linné, 1758) * ''Pomacea q ...
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Antioxidant
Antioxidants are compounds that inhibit oxidation, a chemical reaction that can produce free radicals. This can lead to polymerization and other chain reactions. They are frequently added to industrial products, such as fuels and lubricants, to prevent oxidation, and to foods to prevent spoilage, in particular the rancidification of oils and fats. In cells, antioxidants such as glutathione, mycothiol or bacillithiol, and enzyme systems like superoxide dismutase, can prevent damage from oxidative stress. The only dietary antioxidants are vitamins A, C, and E, but the term ''antioxidant'' has also been applied to numerous other dietary compounds that only have antioxidant properties in vitro, with little evidence for antioxidant properties in vivo. Dietary supplements marketed as antioxidants have not been shown to maintain health or prevent disease in humans. History As part of their adaptation from marine life, terrestrial plants began producing non-marine antioxi ...
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Aposematism
Aposematism is the advertising by an animal to potential predators that it is not worth attacking or eating. This unprofitability may consist of any defences which make the prey difficult to kill and eat, such as toxicity, venom, foul taste or smell, sharp spines, or aggressive nature. These advertising signals may take the form of conspicuous coloration, sounds, odours, or other perceivable characteristics. Aposematic signals are beneficial for both predator and prey, since both avoid potential harm. The term was coined in 1877 by Edward Bagnall Poulton for Alfred Russel Wallace's concept of warning coloration. Aposematism is exploited in Müllerian mimicry, where species with strong defences evolve to resemble one another. By mimicking similarly coloured species, the warning signal to predators is shared, causing them to learn more quickly at less of a cost. A genuine aposematic signal that a species actually possesses chemical or physical defences is not the only way to ...
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